5104789 Monoclonal antibodies which discriminate between strains of citrus tristeza virus

1992 ◽  
Vol 10 (3) ◽  
pp. 501
Keyword(s):  
Monoclonal Antibodies ◽  
Citrus Tristeza Virus ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals In Situ Immunoassay (ISIA) of Field Grapefruit Trees Inoculated with Mild Isolates of Citrus tristeza virus Indicates Mixed Infections with Severe Isolates

Plant Disease ◽  
2002 ◽  
Vol 86 (5) ◽  
pp. 458-461 ◽  
Author(s):  
Youjian Lin ◽  
Phyllis A. Rundell ◽  
Charles A. Powell
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Citrus Tristeza Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mixed Infections ◽  
The Cross ◽  
Tristeza Virus ◽  
Mild Isolate ◽  
Citrus Tristeza

Ten grapefruit trees that had been inoculated with a mild isolate of Citrus tristeza virus (CTV) and maintained in the field for 18 years were found in a previous study to be declining and infected with severe isolates of CTV, or symptomless and infected with mild isolates of CTV, using enzyme-linked immunosorbent assay (ELISA). They were assayed with an in situ immunoassay (ISIA) procedure using monoclonal antibodies 17G11 (reacts with most Florida isolates of CTV) and MCA13 (reacts with severe, but not Florida mild isolates of CTV). All the grapefruit trees were 17G11 positive by ELISA and ISIA. The five trees that showed moderate decline symptoms were MCA13 positive by ELISA and ISIA. The five symptomless trees were MCA13 negative by ELISA. However, four of the five symptomless trees were MCA13 positive by ISIA, which showed that ISIA with MCA13 had greater sensitivity in detecting severe CTV isolates than ELISA. These results suggested that the cross-protected grapefruit trees, regardless of symptoms, were infected with both mild and severe isolates of CTV.

scholarly journals Production and Characterization of Monoclonal Antibodies Specific for Citrus Tristeza Virus and Their Use for Diagnosis

1986 ◽  
Vol 67 (1) ◽  
pp. 91-96 ◽  
Author(s):  
C. Vela ◽  
M. Cambra ◽  
E. Cortes ◽  
P. Moreno ◽  
J. G. Miguet ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Citrus Tristeza Virus ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Differential diagnosis of Brazilian strains of Citrus tristeza virus by epitope mapping of coat protein using monoclonal antibodies

2009 ◽  
Vol 145 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Luís Antonio Peroni ◽  
Márcio Lorencini ◽  
José Raimundo Ribeiro dos Reis ◽  
Marcos Antonio Machado ◽  
Dagmar Ruth Stach-Machado
Keyword(s):  
Differential Diagnosis ◽  
Monoclonal Antibodies ◽  
Coat Protein ◽  
Epitope Mapping ◽  
Citrus Tristeza Virus ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Natural Field Spread of Mild and Severe Isolates of Citrus Tristeza Virus in Florida

Plant Disease ◽  
1997 ◽  
Vol 81 (1) ◽  
pp. 18-20 ◽  
Author(s):  
C. A. Powell ◽  
R. R. Pelosi ◽  
R. C. Bullock
Keyword(s):  
Monoclonal Antibodies ◽  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Sour Orange ◽  
Natural Field ◽  
Average Percentage ◽  
Aphid Control ◽  
Tristeza Virus ◽  
Severe Isolate ◽  
Citrus Tristeza

The effectiveness of five different aphid control regimes in delaying movement of mild and severe isolates of citrus tristeza virus (CTV) into a CTV-free sweet orange scion on sour orange rootstock block was monitored annually for 5 years, using severe isolate-specific and isolate-nonspecific monoclonal antibodies. The average percentage of trees infected with a severe isolate of CTV was 32, 32, 20, 25, and 28 for trees treated with Temik, Temik + Meta-Systox R (MSR), MSR, stylet oil, or no aphid control, respectively, at the conclusion of the experiment (5 years). These percentages were not significantly different (P ≤ 0.05). The average percentage of trees infected only with mild isolates was 13, 10, 7, 7, and 17 for the above treatments, respectively. The infection with mild isolates was significantly greater (P ≤ 0.05) with no aphid control than with stylet oil or MSR treatments.

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