123 Neural degeneration mutants in the zebrafish, danio rerio

Forty zebrafish mutants with localized or general neural degeneration are described. The onset and duration of degeneration and the distribution of ectopically dying cells are specific characteristics of each mutant. Mutants are classified into four groups by these parameters. Class I: late focal neural degeneration mutants. These 18 mutants have restricted cell death mainly in the tectum and the dorsal hindbrain after 36 hours. The degeneration does not spread and disappears at later stages of development. Class II: early focal neural degeneration mutants. Ten mutants in this class exhibit transient restricted degeneration affecting mainly the diencephalon, the hindbrain and the spinal cord at 20 hours. The midbrain is less affected. The degeneration shifts to the dorsal diencephalon and the tectum at 36 hours. Class III: late spreading neural degeneration mutants. The 8 mutants in this class display a degeneration that is first seen in the tectum and subsequently spreads throughout the nervous system from 36 hours on. Class IV: early general neural degeneration mutants. This class of four mutants already shows overall cell degeneration in the nervous system at the 15-somite stage. Three of the class I mutants show a change in the pattern of gene expression in the anlage of a brain structure prior to the onset of degeneration. These results suggest that focal cell death may be a useful clue for the detection of early patterning defects of the vertebrate nervous system in regions devoid of visible landmarks.

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Fluorocitrate Neural Dystrophy of the Guinea Pig Cochlea

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100115468 ◽

1975 ◽

Vol 33 ◽

pp. 368-369

Author(s):

G.J. Spector ◽

C.D. Carr ◽

I. Kaufman Arenberg ◽

R.H. Maisel

Keyword(s):

Hearing Loss ◽

Hair Cells ◽

Sodium Phosphate ◽

Sensory Cells ◽

Barium Salt ◽

Perfusion Medium ◽

Cochlear Hair Cells ◽

Neural Degeneration ◽

Sodium Phosphate Buffer ◽

Cochlear Neurons

All studies on primary neural degeneration in the cochlea have evaluated the end stages of degeneration or the indiscriminate destruction of both sensory cells and cochlear neurons. We have developed a model which selectively simulates the dystrophic changes denoting cochlear neural degeneration while sparing the cochlear hair cells. Such a model can be used to define more precisely the mechanism of presbycusis or the hearing loss in aging man.Twenty-two pigmented guinea pigs (200-250 gm) were perfused by the perilymphatic route as live preparations using fluorocitrate in various concentrations (15-250 ug/cc) and at different incubation times (5-150 minutes). The barium salt of DL fluorocitrate, (C6H4O7F)2Ba3, was reacted with 1.0N sulfuric acid to precipitate the barium as a sulfate. The perfusion medium was prepared, just prior to use, as follows: sodium phosphate buffer 0.2M, pH 7.4 = 9cc; fluorocitrate = 15-200 mg/cc; and sucrose = 0.2M.

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Local interaction rules and collective motion in black neon tetra (Hyphessobrycon herbertaxelrodi) and zebrafish (Danio rerio).

Journal of Comparative Psychology ◽

10.1037/com0000172 ◽

2019 ◽

Vol 133 (2) ◽

pp. 143-155 ◽

Cited By ~ 2

Author(s):

Vicenç Quera ◽

Elisabet Gimeno ◽

Francesc S. Beltran ◽

Ruth Dolado

Keyword(s):

Danio Rerio ◽

Collective Motion ◽

Local Interaction

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Rapid, high-throughput homogenization of embryonic or larval zebrafish (Danio rerio)

Protocol Exchange ◽

10.1038/nprot.2009.211 ◽

2009 ◽

Cited By ~ 3

Author(s):

Carlton Hoyt

Keyword(s):

High Throughput ◽

Danio Rerio ◽

Larval Zebrafish

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Comprehensive spatio-temporal expression profiling reveals a complete natriuretic peptide system in the developing Zebrafish (Danio rerio)

Endocrine Abstracts ◽

10.1530/endoabs.31.p140 ◽

2013 ◽

pp. 1-1

Author(s):

Andrew Lessey ◽

Samantha Mirczuk ◽

Imelda McGonnell ◽

Robert Fowkes

Keyword(s):

Natriuretic Peptide ◽

Danio Rerio ◽

Expression Profiling ◽

Temporal Expression ◽

Peptide System ◽

Spatio Temporal ◽

Natriuretic Peptide System

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Efektivitas Hipoglikemik Fraksi Etil Asetat Ampas Tahu menggunakan Model Hewan Ikan Zebra (Danio rerio)

Jurnal Jamu Indonesia ◽

10.29244/jji.v5i1.105 ◽

2020 ◽

Vol 5 (1) ◽

pp. 16-21

Author(s):

Nina Herlina ◽

Mulyati ◽

Yulianita ◽

Putri Ananda

Keyword(s):

Danio Rerio ◽

Area Under Curve

Ampas tahu mengandung isoflavon genistein dan daidzein yang diduga memiliki efek hipoglikemik. Penelitian ini bertujuan untuk menguji efek hipoglikemik fraksi etil asetat ampas tahu (FEAAT). Ekstraksi isoflavon ampas tahu dilakukan dengan metode refluks menggunakan etanol dan HCl, kemudian fraksinasi menggunakan etil asetat. Efek hipoglikemik FEAAT dibuktikan menggunakan ikan zebra sebagai hewan uji. Kelompok perlakuan terdiri dari kontrol negatif (akuades), FEAAT1 dan FEAAT2 dengan konsentrasi 3,75% dan 5% serta kontrol positif (metformin). Hiperglikemia diinduksi dengan perendaman menggunakan aloksan 0,05% selama 30 menit, glukosa 1% selama 30 menit. Selanjutnya dilakukan pemberian sampel uji selama 120 menit secara berurutan untuk menguji efek hipoglikemik dari sampel uji. Cuplikan darah diambil pada menit ke-0, 30, 60, 90, dan 120 untuk diuji kadar glukosa darah. Hasil uji menunjukkan bahwa terdapat perbedaan yang nyata pada luas Area Under Curve (AUC0-120) kontrol negatif dengan FEAAT2 dan metformin (p < 0,01). Sementara hasil nilai AUC0-120 FEAAT1 tidak berbeda nyata dengan kontrol negatif. FEAAT2 efektif sebagai agen hipoglikemik pada model hewan ikan zebra yang diinduksi hiperglikemik.

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