Uridine and cytidine nucleotide synthesis in renal hypertrophy: Biochemical differences in response to the growth stimulus of diabetes and unilateral nephrectomy

1992 ◽  
Vol 47 (2) ◽  
pp. 168-180 ◽  
Author(s):  
Sirilaksana Kunjara ◽  
Milena Sochor ◽  
Murad Ali ◽  
Michael Bennett ◽  
A.Leslie Greenbaum ◽  
...  
Keyword(s):  
Unilateral Nephrectomy ◽  
Renal Hypertrophy ◽  
Nucleotide Synthesis

scholarly journals Concentration of phosphoribosyl pyrophosphate in renal hypertrophy. Contrasting effects of early diabetes and unilateral nephrectomy

1986 ◽  
Vol 239 (1) ◽  
pp. 241-244 ◽  
Author(s):  
S Kunjara ◽  
M Sochor ◽  
A L Greenbaum ◽  
P McLean
Keyword(s):  
Nucleic Acid ◽  
Unilateral Nephrectomy ◽  
Phosphoribosyl Pyrophosphate ◽  
Renal Hypertrophy ◽  
Early Diabetes ◽  
Nucleotide Synthesis ◽  
Contralateral Kidney ◽  
Increment Of Growth ◽  
Renal Enlargement

Studies were made of the renal phosphoribosyl pyrophosphate (PPRibP) content and PPRibP synthetase (EC 2.7.6.1) activity in rats diabetic for 5, 14 or 20 days, or unilaterally nephrectomized (UN) for 5 days, and in doubly lesioned animals. Approximately equal degrees of renal enlargement were found after 5 days diabetes or 5 days UN. In the doubly lesioned animals the increment of growth was additive. Unilateral nephrectomy of 5 days duration, in contrast with diabetes, had no effect on the PPRibP content of the contralateral kidney, nor did it modify the renal PPRibP content when performed on animals diabetic for 5, 14 or 20 days. The activity of PPRibP synthetase was unaffected by diabetes, UN or diabetes +UN. The results pinpoint a stage of nucleotide synthesis which is differentially affected by the two stimuli, in line with evidence for differences in regulation of nucleic acid turnover in the two conditions.

scholarly journals THE DEGREE OF COMPENSATORY RENAL HYPERTROPHY FOLLOWING UNILATERAL NEPHRECTOMY

1938 ◽  
Vol 67 (4) ◽  
pp. 515-519 ◽  
Author(s):  
Lois L. MacKay ◽  
T. Addis ◽  
Eaton M. MacKay
Keyword(s):  
Protein Intake ◽  
Compensatory Hypertrophy ◽  
Unilateral Nephrectomy ◽  
Albino Rats ◽  
Renal Hypertrophy ◽  
Young Rats ◽  
Compensatory Renal Hypertrophy ◽  
Old Rats

Compensatory hypertrophy of the kidney in albino rats is increased by an increase in the protein intake. The effect is greater in old rats than young rats. Successive increases in the protein intake are followed by a reduction in the increase in the degree of compensatory renal hypertrophy.

scholarly journals Nitric Oxide in Serum and Renal Tissue During Compensatory Renal Hypertrophy in Rats

2006 ◽  
Vol 6 (1) ◽  
pp. 46-49 ◽  
Author(s):  
Jasminko Huskić ◽  
Asija Začiragić ◽  
Nermina Babić ◽  
Nedžad Mulabegović
Keyword(s):  
Nitric Oxide ◽  
Renal Tissue ◽  
Unilateral Nephrectomy ◽  
Hemodynamic Changes ◽  
Renal Hypertrophy ◽  
Griess Reaction ◽  
Reaction Conversion ◽  
Compensatory Renal Hypertrophy ◽  
Male Wistar Rats ◽  
Dorsolateral Approach

Nitric oxide (NO) level in serum and renal tissue has been examined in 15 male Wistar rats, body weight 200-250 g, 7 days after unilateral nephrectomy. All rats were ether-anaesthetized and the kidneys were removed by dorsolateral approach. NO concentration in serum and renal tissue was determined by classic colorimetric Griess reaction. Conversion of NO(3)(2-) into NO(2)(2-) was done with elementary zinc. Results have shown that NO concentration in renal tissue is statistically higher in rats 7 days after unilateral nephrectomy then in control renal tissue before compensatory kidney growth (p<0,02). There is no difference between NO concentration in serum before unilateral nephrectomy and 7 days after nephrectomy. These findings suggest that NO may play an important role in mediating the hemodynamic changes associated with reduced renal mass.

scholarly journals Renal hypertrophy in experimental diabetes. The activity of the ‘de novo’ and salvage pathways of purine [corrected] synthesis

1988 ◽  
Vol 249 (3) ◽  
pp. 911-914 ◽  
Author(s):  
S Kunjara ◽  
S J Beardsley ◽  
A L Greenbaum
Keyword(s):  
Nucleic Acids ◽  
De Novo ◽  
Experimental Diabetes ◽  
Salvage Pathway ◽  
Phosphoribosyl Pyrophosphate ◽  
Renal Hypertrophy ◽  
Early Diabetes ◽  
Nucleotide Synthesis ◽  
Main Route ◽  
Salvage Pathways

Measurements were made of the activity of phosphoribosyl pyrophosphate amidotransferase (PPRibP-At, EC 2.4.2.14) and of adenine (APRT, EC 2.4.2.7) and hypoxanthine (HPRT, EC 2.4.2.8) phosphoribosyltransferases, representing the ‘de novo’ and salvage pathways respectively. PPRibP-At activity increased within 3 days of diabetes, whereas APRT and HPRT increased later. Incorporation of [14C]formate and of [8-14C]adenine into the nucleic acids of kidney slices showed that formate was incorporated earlier, and to a greater extent, than was adenine. These results indicate that, although the ‘de novo’ pathway for nucleotide synthesis is the main route in early diabetes, the salvage pathway assumes greater importance at later stages.

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