Matrix solid phase dispersion (MSPD) extraction and liquid chromatographic determination of five benzimidazole anthelmintics in pork muscle tissue

1990 ◽  
Vol 3 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Austin R. Long ◽  
Lily C. Hsieh ◽  
Marsha S. Malbrough ◽  
Charles R. Short ◽  
Steven A. Barker
Keyword(s):  
Muscle Tissue ◽  
Solid Phase ◽  
Chromatographic Determination ◽  
Matrix Solid Phase Dispersion ◽  
Phase Dispersion ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Benzimidazole Anthelmintics

Matrix Solid-Phase Dispersion Isolation and Liquid Chromatographic Determination of Oxolinic Acid in Channel Catfish (Ictalurus punctatus) Muscle Tissue

1992 ◽  
Vol 75 (3) ◽  
pp. 428-432 ◽  
Author(s):  
Herman H Jarboe ◽  
Kevin M Kleinow
Keyword(s):  
Muscle Tissue ◽  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Solid Phase ◽  
Chromatographic Determination ◽  
Oxolinic Acid ◽  
Matrix Solid Phase Dispersion ◽  
Phase Dispersion ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Matrix solid-phase dispersion Isolation and liquid chromatographic techniques were developed to quantify oxolinic acid (OA) and OA-related metabolites in channel catfish (Ictalurus punctatus) muscle tissue and bile. Mean percent recovery, correlation coefficient, and inter- and intra-assay variabilities were 82.8 ±15.0%, 0.996 ±0.004,12.5 ±8.9%, and 1.22%, respectively, for OA Isolated from fortified muscle tissue. Using the methodologies described in the current study, incurred OA In muscle tissue and 4 OA-related metabolites were Isolated In the bile of dosed catfish.

scholarly journals Liquid Chromatographic Determination of Mebendazole and its Metabolites, Aminomebendazole and Hydroxymebendazole, in Eel Muscle Tissue

1996 ◽  
Vol 79 (3) ◽  
pp. 645-651 ◽  
Author(s):  
Christiaan A J Hajee ◽  
Nel Haagsma
Keyword(s):  
Muscle Tissue ◽  
Mobile Phase ◽  
Solid Phase ◽  
Chromatographic Determination ◽  
Extraction Column ◽  
Phase Extraction ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract An analytical method is presented for liquid chromatographic (LC) determination of mebendazole (MBZ), hydroxymebendazole (MBZ-OH), and aminomebendazole (MBZ-NH2) in eel muscle tissue. Muscle tissue is extracted with ethyl acetate at pH 7.5. After addition of n-hexane, the extract is cleaned up and concentrated on an aminopropyl solid-phase extraction column. The test solutions are analyzed isocratically on a ChromSpher B LC column with acetonitrile–phosphate buffer, pH 6.2, as mobile phase. Limits of detection and quantitation were 0.7 and 1.1 ¼g/kg, respectively, for MBZOH; 1.4 and 2.3 ¼g/kg, respectively, for MBZ; and 1.5 and 2.1 ¼g/kg, respectively, for MBZ-NH2. Interand intraday coefficients of variation were 3.5 and 3.4%, respectively, for MBZ-OH; 2.5 and 3.1%, respectively, for MBZ; and 5.8 and 4.8%, respectively, for MBZ-NH2. Mean recoveries were 90% for MBZ, 74% for MBZ-NH2, and 92% for MBZ-OH. A linear range of applicability of at least 10–1000 ¼g/kg was found for each analyte. Incurred MBZ-NH2 (181.3 ¼g/kg) was identified in eel muscle tissue apart from MBZ (23.7 ¼g/kg) after 48 h exposure ina treatment bath containing MBZ at 1 mg/L.

Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of Sulfadimethoxine in Catfish (Ictalurus punctatus) Muscle Tissue

1990 ◽  
Vol 73 (6) ◽  
pp. 868-871 ◽  
Author(s):  
Austin R Long ◽  
Lily C Hsieh ◽  
Marsha S Malbrou ◽  
Charles R Short ◽  
Steven A Barker
Keyword(s):  
Muscle Tissue ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Fish Muscle ◽  
Tissue Samples ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method for the isolation and liquid chromatographic determination of sulfadimethoxine In catfish (Ictalurus punctatus) muscle tissue Is presented. Blank control and sulf adlmethox- Ine-fortlfled fish muscle tissue samples (0.5 g) were blended with octadecylsllyl (C18,40 /μm, 18% load, endcapped) derivatlzed silica packing material. A column made from the C18/ fish tissue blend was first washed with hexane (8 mL), following which the sulfadimethoxine was eluted with dlchloromethane (8 mL). The eluant contained sulfadimethoxine analyte that was free from Interfering compounds when analyzed by liquid chromatography with UV detection (photodlode array, 270 nm). Standard curves for sulfadimethoxine Isolated from fortified samples were linear (0.999 ± 0.001) with an average relative percentage recovery of 101.1 ± 4.2% for the concentration range (50, 100, 200,400, 800, and 1600 ng/g) examined using sulfamethoxazole as the Internal standard. The interassay variability was 10.7 ± 8.2% with an Intra-assay variability of 2.2%.

Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of Oxytetracycline in Catfish (Ictalurus punctatus) Muscle Tissue

1990 ◽  
Vol 73 (6) ◽  
pp. 864-867 ◽  
Author(s):  
Austin R Long ◽  
Lily C Hsiesh ◽  
Marsha S Malbrough ◽  
Charles R Short ◽  
Steven A Barker
Keyword(s):  
Muscle Tissue ◽  
Ictalurus Punctatus ◽  
Solid Phase ◽  
Chromatographic Determination ◽  
Fish Muscle ◽  
Butylated Hydroxytoluene ◽  
Tissue Samples ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method for Isolation and liquid chromatographic determination of oxytetracycllne In catfish (Ictalurus punctatus) muscle tissue Is presented. Blank control and oxytetracycllne- fortlfied fish muscle tissue samples (0.5 g) were blended with octadecylsilyl (C18, 40 μm, 18% load, endcapped) derlvatized silica packing material (2 g) containing 0.05 g each of oxalic acid and disodlum ethylenedlamlnetetraacetate. A column made from the C18/fish tissue matrix was first washed with hexane (8 mL), following which the oxytetracycllne was eluted with acetonltrile-methanol (1 + 1, v/v) containing 0.06% w/v each of butylated hydroxyanlsole and butylated hydroxytoluene. The eluate contained oxytetracycllne analyte that was free from Interfering compounds when analyzed by liquid chromatography with UV detection (photodlode array set at 365 nm). Standard curves for oxytetracycllne Isolated from fortified samples were linear (0.998 ± 0.002) with an average absolute percentage recovery of 80.9 ± 6.6% for the concentration range (50,100,200, 400, 800, 1600, and 3200 ng/g) examined. The Interassay variability was 11.3 ± 5.2% with an Intra-assay variability of 1.1%.

Matrix Solid Phase Dispersion Isolation and Liquid Chromatographic Determination of Five Benzimidazole Anthelmintics in Fortified Beef Liver

1990 ◽  
Vol 73 (6) ◽  
pp. 860-863 ◽  
Author(s):  
Austin R Long ◽  
Marsha S Malbrough ◽  
Lily C Hsieh ◽  
Charles R Short ◽  
Steven A Barker
Keyword(s):  
Solid Phase ◽  
Correlation Coefficients ◽  
Chromatographic Determination ◽  
Activated Alumina ◽  
Beef Liver ◽  
Liquid Chromatographic Determination ◽  
Internal Standardization ◽  
Liquid Chromatographic ◽  
Benzimidazole Anthelmintics

Abstract A multlresidue method for Isolation and liquid chromatographic determination of 5 benzimidazole anthelmintics (thiabendazole, oxfendazole, mebendazole, albendazole, and fenbendazole) in beef liver tissue is presented. Blank or benzlmldazole-fortlfled liver samples (0.5 g) were blended with octadecylsilyl derlvatlzed silica packing material (C18, 18% load, endcapped, 2 g). A column made from the C18/ liver matrix was first washed with hexane (8 ml_), following which the benzlmldazoles were eluted with acetonltrile. The acetonltrlle extract was then passed through an activated alumina column. The eluate contained benzimidazole analytes that were free from Interfering compounds as determined by UV detection (photodlode array, 290 nm). Correlation coefficients of standard curves for Individual benzlmldazoles Isolated from fortified samples, using internal standardization, were linear (0.996 ± 0.002 to 0.999 ± 0.001) with average relative percentage recoveries from 62.0 ± 6.7 to 86.8 ± 8.6% for the concentration range (100- 3200 ng/g) examined. The interassay variability was 7.0 ± 4.1 to 12.9 ± 10.2% with an Intra-assay variability from 2.2 to 4.0%.

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