Cyclic AMP phosphodiesterase activity in peripheral blood mononuclear leukocytes in patients with atopic dermatitis

1990 ◽  
Vol 1 (3) ◽  
pp. 233
Author(s):  
T. Sawai ◽  
K. Ikai ◽  
M. Uehara
Keyword(s):  
Atopic Dermatitis ◽  
Cyclic Amp ◽  
Peripheral Blood ◽  
Mononuclear Leukocytes ◽  
Phosphodiesterase Activity ◽  
Peripheral Blood Mononuclear ◽  
Cyclic Amp Phosphodiesterase

Arachidonic acid and LTB4 enhance aggregation of psoriatic peripheral blood mononuclear leukocytes in vitro

1987 ◽  
Vol 279 (5) ◽  
pp. 347-350 ◽  
Author(s):  
P. D. Pigatto ◽  
M. M. Polengni ◽  
G. F. Altomare ◽  
G. L. Tadini ◽  
S. Villa
Keyword(s):  
Arachidonic Acid ◽  
Peripheral Blood ◽  
Mononuclear Leukocytes ◽  
Peripheral Blood Mononuclear

Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae

1987 ◽  
Vol 7 (10) ◽  
pp. 3629-3636
Author(s):  
J Nikawa ◽  
P Sass ◽  
M Wigler
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cyclic Amp ◽  
Copy Number ◽  
Growth Arrest ◽  
Yeast Cells ◽  
Phosphodiesterase Activity ◽  
Camp Phosphodiesterase ◽  
High Affinity ◽  
Cyclic Amp Phosphodiesterase

Saccharomyces cerevisiae contains two genes which encode cyclic AMP (cAMP) phosphodiesterase. We previously isolated and characterized PDE2, which encodes a high-affinity cAMP phosphodiesterase. We have now isolated the PDE1 gene of S. cerevisiae, which encodes a low-affinity cAMP phosphodiesterase. These two genes represent highly divergent branches in the evolution of phosphodiesterases. High-copy-number plasmids containing either PDE1 or PDE2 can reverse the growth arrest defects of yeast cells carrying the RAS2(Val-19) mutation. PDE1 and PDE2 appear to account for the aggregate cAMP phosphodiesterase activity of S. cerevisiae. Disruption of both PDE genes results in a phenotype which resembles that induced by the RAS2(Val-19) mutation. pde1- pde2- ras1- ras2- cells are viable.

Sign in / Sign up

Export Citation Format

Share Document