Amino acid sequence motif of group I intron endonucleases is conserved in open reading frames of group II introns

1994 ◽  
Vol 19 (10) ◽  
pp. 402-404 ◽  
Author(s):  
David A. Shub ◽  
Heidi Goodrich-Blair ◽  
Sean R. Eddy
1991 ◽  
Vol 276 (3) ◽  
pp. 841-843 ◽  
Author(s):  
T D H Bugg ◽  
P R Alefounder ◽  
C Abell

1996 ◽  
Vol 271 (48) ◽  
pp. 30410-30416 ◽  
Author(s):  
Nick Menhart ◽  
Tracy Mitchell ◽  
Denise Lusitani ◽  
Nancy Topouzian ◽  
W.-M.L. Fung

1999 ◽  
Vol 65 (12) ◽  
pp. 5198-5206 ◽  
Author(s):  
Toru Shigematsu ◽  
Satoshi Hanada ◽  
Masahiro Eguchi ◽  
Yoichi Kamagata ◽  
Takahiro Kanagawa ◽  
...  

ABSTRACT The soluble MMO (sMMO) gene clusters from group I methanotrophs were characterized. An 8.1-kb KpnI fragment fromMethylomonas sp. strain KSWIII and a 7.5-kbSalI fragment from Methylomonas sp. strain KSPIII which contained the sMMO gene clusters were cloned and sequenced. The sequences of these two fragments were almost identical. The sMMO gene clusters in the fragment consisted of six open reading frames which were 52 to 79% similar to the corresponding genes of previously described sMMO gene clusters of the group II and group X methanotrophs. The phylogenetic analysis of the predicted amino acid sequences of sMMO demonstrated that the sMMOs from these strains were closer to that from M. capsulatus Bath in the group X methanotrophs than to those from Methylosinus trichosporiumOB3b and Methylocystis sp. strain M in the group II methanotrophs. Based on the sequence data of sMMO genes of our strains and other methanotrophs, we designed a new PCR primer to amplify sMMO gene fragments of all the known methanotrophs harboring themmoX gene. The primer set was successfully used for detecting methanotrophs in the groundwater of trichloroethylene-contaminated sites during in situ-biostimulation treatments.


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