Targeting peptide nucleic acid-protein conjugates to structural features within duplex DNA

1995 ◽  
Vol 3 (4) ◽  
pp. 437-445 ◽  
Author(s):  
James C. Norton ◽  
John H. Waggenspack ◽  
Elana Varnum ◽  
David R. Corey
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Structural Features ◽  
Duplex Dna ◽  
Protein Conjugates ◽  
Acid Protein ◽  
Targeting Peptide

scholarly journals Strand-invasion of duplex DNA by peptide nucleic acid oligomers.

1993 ◽  
Vol 90 (22) ◽  
pp. 10648-10652 ◽  
Author(s):  
N. J. Peffer ◽  
J. C. Hanvey ◽  
J. E. Bisi ◽  
S. A. Thomson ◽  
C. F. Hassman ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Duplex Dna ◽  
Strand Invasion

scholarly journals Structural Insights on Tiny Peptide Nucleic Acid (PNA) Analogues of miRNA-34a: An in silico and Experimental Integrated Approach

2020 ◽  
Vol 8 ◽  
Author(s):  
Maria Moccia ◽  
Flavia Anna Mercurio ◽  
Emma Langella ◽  
Valerio Piacenti ◽  
Marilisa Leone ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Uv Spectroscopy ◽  
Integrated Approach ◽  
Structural Features ◽  
Seed Region ◽  
Rna Sequences ◽  
Nmr Characterization ◽  
Rna Targeting ◽  
Fluorescent Tag

In the present work, structural features of the interaction between peptide nucleic acid (PNA)-based analogs of the tumor-suppressor microRNA-34a with both its binding sites on MYCN mRNA were investigated. In particular, the region from base 1 to 8 (“seed” region) of miR-34a was reproduced in the form of an 8-mer PNA fragment (tiny PNA), and binding to target 3'UTR MYCN mRNA, was studied by a seldom reported and detailed NMR characterization, providing evidence for the formation of anti-parallel duplexes with a well-organized structural core. The formation of PNA-3'UTR duplexes was also confirmed by Circular Dichroism, and their melting curves were measured by UV spectroscopy. Nevertheless, this study offered a valuable comparison between molecular dynamics predictions and experimental evidence, which showed great correlation. Preliminary uptake assays were carried out in Neuroblastoma Kelly cells, using short peptide conjugates as carriers and FITC fluorescent tag for subcellular localization. Moderate internalization was observed without the use of transfecting agents. The reported results corroborate the interest toward the design and development of chimeric PNA/RNA sequences as effective RNA-targeting agents.

scholarly journals Electron microscopy mapping of oligopurine tracts in duplex DNA by peptide nucleic acid targeting

1994 ◽  
Vol 22 (24) ◽  
pp. 5218-5222 ◽  
Author(s):  
Vadim V. Demidov ◽  
Dmitry I. Cherny ◽  
Alexey V. Kurakin ◽  
Michael V. Yavnilovich ◽  
Vladislav A. Malkov ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Duplex Dna

scholarly journals Kinetics and mechanism of polyamide ("peptide") nucleic acid binding to duplex DNA.

1995 ◽  
Vol 92 (7) ◽  
pp. 2637-2641 ◽  
Author(s):  
V. V. Demidov ◽  
M. V. Yavnilovich ◽  
B. P. Belotserkovskii ◽  
M. D. Frank-Kamenetskii ◽  
P. E. Nielsen
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Kinetics And Mechanism ◽  
Duplex Dna ◽  
Nucleic Acid Binding

scholarly journals Insights into peptide nucleic acid (PNA) structural features: The crystal structure of a D-lysine-based chiral PNA-DNA duplex

2003 ◽  
Vol 100 (21) ◽  
pp. 12021-12026 ◽  
Author(s):  
V. Menchise ◽  
G. De Simone ◽  
T. Tedeschi ◽  
R. Corradini ◽  
S. Sforza ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Structural Features ◽  
Dna Duplex

Targeting linear duplex DNA with mixed-base peptide nucleic acid oligomers facilitated by bisPNA openers

2007 ◽  
Vol 362 (1) ◽  
pp. 145-147 ◽  
Author(s):  
Igor G. Panyutin ◽  
Irina V. Panyutin ◽  
Vadim V. Demidov
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Duplex Dna
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