Phytochrome regulates phosphorylation of a protein with characteristics of a nucleoside diphosphate kinase in the crude membrane fraction from stem sections of etiolated pea seedlings

1996 ◽  
Vol 33 (2) ◽  
pp. 143-151 ◽  
Author(s):  
Tohru Hamada ◽  
Norifumi Tanaka ◽  
Takafumi Noguchi ◽  
Narimichi Kimura ◽  
Kohji Hasunuma
Keyword(s):  
Membrane Fraction ◽  
Nucleoside Diphosphate Kinase ◽  
Crude Membrane ◽  
Pea Seedlings ◽  
Crude Membrane Fraction

Binding of muscimol and GABA in sub-fractions of a crude membrane fraction of rat brain

1980 ◽  
Vol 29 (7) ◽  
pp. 1077-1079 ◽  
Author(s):  
V. Varga ◽  
F.V. DeFeudis ◽  
L. Ossola ◽  
M. Geffard ◽  
P. Mandel
Keyword(s):  
Rat Brain ◽  
Membrane Fraction ◽  
Crude Membrane ◽  
Crude Membrane Fraction

Characterization of glycyrrhizin-binding protein kinase from the crude membrane fraction of rat liver

1990 ◽  
Vol 167 (3) ◽  
pp. 876-882 ◽  
Author(s):  
Akira Ishikawa ◽  
Ryunosuke Kanamaru ◽  
Akira Wakui ◽  
Shin-ichiro Kanno ◽  
Kenzo Ohtsuki
Keyword(s):  
Protein Kinase ◽  
Rat Liver ◽  
Membrane Fraction ◽  
Binding Protein ◽  
Crude Membrane ◽  
Crude Membrane Fraction

scholarly journals THE ISOLATION OF A CELL MEMBRANE FRACTION FROM RAT LIVER

1960 ◽  
Vol 8 (2) ◽  
pp. 413-422 ◽  
Author(s):  
David M. Neville
Keyword(s):  
Rat Liver ◽  
Membrane Fraction ◽  
Cell Membranes ◽  
Cold Water ◽  
Sucrose Solution ◽  
Nuclear Membranes ◽  
A Cell ◽  
Liver Homogenates ◽  
Cell Fragments ◽  
Crude Membrane Fraction

A procedure is described for isolating cell membranes from rat liver homogenates. 20 gm. of rat liver was homogenized in a Dounce homogenizer in ice cold water buffered to pH 7.5 with NaHCO3, rupturing all of the cells and most nuclei. The diluted homogenate was filtered through cheesecloth to remove precipitated nucleoprotein and centrifuged at 1500 g, 10 minutes, to sediment a crude membrane fraction. The membrane containing sediment was recentrifuged 3 times in conical tubes (1220 g, 10 minutes), the top layer of the 2-layered sediment being retained. Flotation in a sucrose solution d = 1.22 freed the preparation from contaminating cell fragments and nuclear membranes not previously disintegrated. The floating material ∼0.4 ml. was quite homogeneous and consisted of thin amorphous membranes. Electron micrographs revealed numerous double profiles similar in shape and dimensions to apposed liver cell membranes in intact tissue.

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