Metabolism and Characterization of Insect Cell Cultures

Biochemistry ◽  
1985 ◽  
pp. 547-570 ◽  
Author(s):  
W.F. HINK ◽  
D.A. RALPH ◽  
K.H. JOPLIN
Keyword(s):  
Cell Cultures ◽  
Insect Cell ◽  
Insect Cell Cultures

Expression and characterization of diacylglycerol acyltransferase from Arabidopsis thaliana in insect cell cultures

2000 ◽  
Vol 28 (6) ◽  
pp. 687-689 ◽  
Author(s):  
D. H. Hobbs ◽  
M. J. Hills
Keyword(s):  
Arabidopsis Thaliana ◽  
Hydroxy Group ◽  
Cell Cultures ◽  
Insect Cell ◽  
Expression System ◽  
Diacylglycerol Acyltransferase ◽  
Dgat Activity ◽  
Gene Encoding ◽  
Insect Cell Cultures

Diacylglycerol acyltransferase (DGAT) catalyses the acylation of the sn-3 hydroxy group of sn-1,2-diacylglycerol using acyl-CoA. The gene encoding DGAT from Arabidopsis thaliana has been cloned and the function of the enzyme proved by expression of the coding sequence using a bacculovirus expression system in insect cell cultures. The expressed protein catalysed the synthesis of [14C]triacylglycerol from [14C]diacylglycerol and oleoyl-CoA. The heterologously expressed DGAT activity was found mostly associated with the 100000g pellet. The optimum activity was achieved at a neutral pH, in the presence of Mg2+, and at an optimum oleoyl-CoA concentration of 20 μM. The DGAT used the substrates palmitoyl-CoA and oleoyl-CoA equally effectively. In these experiments, the inclusion of recombinant acyl-CoA binding protein had a relatively small effect upon DGAT activity.

scholarly journals Improved method for the production of insect cell cultures in large volume

In Vitro ◽  
1981 ◽  
Vol 17 (8) ◽  
pp. 744-744 ◽  
Author(s):  
S. A. Weiss ◽  
G. C. Smith ◽  
S. S. Kalter ◽  
J. L. Vaughn
Keyword(s):  
Cell Cultures ◽  
Large Volume ◽  
Insect Cell ◽  
Improved Method ◽  
Insect Cell Cultures
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