Cell Renewal and Mitogenic Activity of Testosterone in Male Sex Accessory Glands

The study aims to reveal the morphology of the male sex accessory glands of the pangolin at macroscopic and microscopic levels. Macroscopic observation included measurement of length and thickness of each accessory gland while microscopic observation, sample of each accessory gland was processed by histology technique with paraffin method and sliced with 3-5 ?m thickness and stained with hematoxylin-eosin (HE) staining for general structural observation, coloration of alcian blue (AB) pH 2.5 and periodic acid Schiff (PAS) to observe the distribution of acid and neutral mucopolysaccharides in each glands. The results showed that the male sex accessory glands of the pangolin consist of vesicular gland and prostate, and bulbourethral gland which were not observed macroscopically. The average length and thickness of vesicular gland were 1.07 cm and 0.41 cm, while the prostate was 1.17 cm and 0.54 cm respectively. All accessory glands were lobulated and separated with a thick connective tissue into lobes and lobules. Acinar cells in the vesicular glands were a serous type, whereas acinar cells in the prostate and bulbourethral gland were the mucous types. Secretion of vesicular gland contains neutral mucopolysaccharide with low concentrations and prostate containing neutral mucopolysaccharide with moderate conJurnal Veteriner Maret 2019 Vol. 20 No. 1 : 38 - 47 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.38 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/201639 centrations, and did not secrete acid mucopolysaccharide. Secretion of bulbourethral glands contains neutral and acidic mucopolysaccharide with strong concentrations. Macroscopically the bulbourethral gland is not observed but has a high carbohydrate which acts as to produce of cement plasma and rinsing urethra from urine.

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Embryology, Anatomy, and Histology of the Male Sex Accessory Glands

Male Accessory Sex Organs ◽

10.1016/b978-0-12-125650-0.50006-5 ◽

1974 ◽

pp. 3-15 ◽

Cited By ~ 8

Author(s):

ROBERTO NARBAITZ

Keyword(s):

Accessory Glands ◽

Male Sex

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Metabolic activity in tissue transplants. Hormone-induced formation of fructose and citric acid in transplants from accessory glands of reproduction

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1949.0037 ◽

1949 ◽

Vol 136 (884) ◽

pp. 461-471 ◽

Cited By ~ 9

Keyword(s):

Citric Acid ◽

Seminal Vesicle ◽

Hormone Treatment ◽

Female Rats ◽

Accessory Glands ◽

Tissue Transplants ◽

Male Sex ◽

Coagulating Gland ◽

Metabolic Behaviour

A combined chemical and cytological study of the behaviour of transplants from certain accessory glands of reproduction in the rat was carried out. It was found that subcutaneous transplants of coagulating gland and seminal vesicle were capable of producing considerable amounts of fructose and citric acid in total anatomical separation from the male reproductive system. In transplants containing coagulating gland and seminal vesicle tissue both fructose and citric acid were formed. In those from coagulating gland alone only fructose was produced, but citric acid was absent. In this respect the metabolic behaviour of the grafts was identical with that of the intact organs. Following castration, coagulating gland transplants lost their ability to form fructose. This was fully restored by treatment with testosterone propionate. Upon cessation of the hormone treatment the process of fructose formation in the transplants was again brought to a standstill. Grafts of coagulating gland could be successfully grown in female rats and brought to a state of fructose secretion by subjecting the female hosts to injections of male sex hormone. The post-castrate retrogressive changes as well as the hormone-induced recovery symptoms were studied in the transplants parallel with similar changes in the intact glands in situ . The chemical findings were corroborated by the histological examination.

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Localization of certain phosphatases in the male sex accessory glands of Suncus murinus sindensis, Anderson, the common shrew

Acta Histochemica ◽

10.1016/s0065-1281(76)80106-7 ◽

1976 ◽

Vol 56 (2) ◽

pp. 175-179

Author(s):

Rajendra P. Goyal ◽

Rajendra S. Mathur

Keyword(s):

Common Shrew ◽

Suncus Murinus ◽

Accessory Glands ◽

The Common ◽

Male Sex

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THE EFFECT OF TESTOSTERONE ON CELL RENEWAL AND MITOTIC CYCLES IN SEX ACCESSORY GLANDS OF CASTRATED MICE

Acta Endocrinologica ◽

10.1530/acta.0.0580696 ◽

1968 ◽

Vol 58 (4) ◽

pp. 696-704 ◽

Cited By ~ 23

Author(s):

Pentti Tuohimaa ◽

Mikko Niemi

Keyword(s):

Small Intestine ◽

Seminal Vesicle ◽

Mitotic Index ◽

Specific Activity ◽

Ventral Prostate ◽

Cell Renewal ◽

Reference Tissue ◽

Accessory Glands ◽

Hormone Injection ◽

Proximal Small Intestine

ABSTRACT The effect of testosterone propionate on cell renewal rate and mitotic cycle of castrated mice was investigated. 0.25 mg of the hormone was given thrice at 24 h intervals and 50 μc of 3H-thymidine was injected simultaneously with the last hormone injection. After this the mice were killed at 1 to 2 h intervals. Ventral prostates and seminal vesicles were weighed and the thymidine incorporation into their epithelia determined in radioautograms. A piece of proximal small intestine was used as reference tissue. The labelling index in the castrated control animals was 1.3 for the seminal vesicle; 68 h after testosterone treatment it was 44. The figures for the ventral prostate were 1.9 and 37, and for the small intestine 44 and 57. The mitotic index in the prostate of the experimental animals and that in the intestine of all the animals was 0.5; in the seminal vesicle it was 0.4. The mitotic index in the accessory glands of the control mice was very low. The specific activity of DNA per nucleus was higher in the hormone treated than in the control animals. The cell cycle (TC) in the prostate was shortened from about 900 h to 20 h after testosterone. The synthetic phase (TS) was shortened from 16 to 7 h, presynthetic (TG1) phase from 900 to 10 h, whereas the postsynthetic phase (TG2) remained unchanged. The corresponding figures for the seminal vesicle were: TG2 from 4.5 to 0.5 h, TS from 12 to 7 h, TG1 from 900 to 10 h and TC from 900 to 18 h. The changes in the intestinal epithelium were less marked: TC from 22 to 16 h, TS from 8.5 to 8 h, TG1 from 12 to 6 h, while TG2 remained unchanged.

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