scholarly journals Morfologi Kelenjar Aksesori Kelamin Jantan pada Trenggiling (Manis javanica) (MORPHOLOGY OF THE MALE SEX ACCESSORY GLANDS OF THE PANGOLIN (MANIS JAVANICA))

2019 ◽  
Vol 20 (1) ◽  
pp. 38
Author(s):  
Yusrizal Akmal ◽  
Chairun Nisa ◽  
Savitri Novelina
Keyword(s):  
Average Length ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Accessory Gland ◽  
Periodic Acid Schiff ◽  
Accessory Glands ◽  
Low Concentrations ◽  
Manis Javanica ◽  
Macroscopic Observation ◽  
Male Sex

The study aims to reveal the morphology of the male sex accessory glands of the pangolin at macroscopic and microscopic levels. Macroscopic observation included measurement of length and thickness of each accessory gland while microscopic observation, sample of each accessory gland was processed by histology technique with paraffin method and sliced with 3-5 ?m thickness and stained with hematoxylin-eosin (HE) staining for general structural observation, coloration of alcian blue (AB) pH 2.5 and periodic acid Schiff (PAS) to observe the distribution of acid and neutral mucopolysaccharides in each glands. The results showed that the male sex accessory glands of the pangolin consist of vesicular gland and prostate, and bulbourethral gland which were not observed macroscopically. The average length and thickness of vesicular gland were 1.07 cm and 0.41 cm, while the prostate was 1.17 cm and 0.54 cm respectively. All accessory glands were lobulated and separated with a thick connective tissue into lobes and lobules. Acinar cells in the vesicular glands were a serous type, whereas acinar cells in the prostate and bulbourethral gland were the mucous types. Secretion of vesicular gland contains neutral mucopolysaccharide with low concentrations and prostate containing neutral mucopolysaccharide with moderate conJurnal Veteriner Maret 2019 Vol. 20 No. 1 : 38 - 47 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.38 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/201639 centrations, and did not secrete acid mucopolysaccharide. Secretion of bulbourethral glands contains neutral and acidic mucopolysaccharide with strong concentrations. Macroscopically the bulbourethral gland is not observed but has a high carbohydrate which acts as to produce of cement plasma and rinsing urethra from urine.   

scholarly journals Anatomy Accessory Glands Of Male Reproductive Of Javan Pangolin (Manis javanica)

2019 ◽  
Author(s):  
Yusrizal Akmal ◽  
Muliari ◽  
Chairun Nisa ◽  
Savitri Novelina
Keyword(s):  
Seminal Plasma ◽  
Microscopic Observation ◽  
Average Length ◽  
Prostate Gland ◽  
Reproductive Organs ◽  
Accessory Gland ◽  
Accessory Glands ◽  
Reproduction Process ◽  
Manis Javanica ◽  
Conical Structure

This study aimed to learn the morphology accessory glands of male reproductive of pangolin by macroanatomy. Male reproductive organs of a pangolin were used in this study. Morphometric observations included measurements of length, diameter/width of the accesoriae gland were analyzed descriptively. Results of microscopic observation showed that the vesicularis glands had a pair laid at the base of the ampulla dexter and sinister that led to the urethra in the form of an enlarged conical with the width at the caudal and protruding toward ventrad, with the average length and thickness is 1.07 cm and 0.41 cm. There was prostata when the conical structure met both, observed enlargement of the base of the urethra were also prominent in the direction ventrad. The length and thickness of the prostata pangolin was 1.17 cm and 0.54 cm. In macroanatomy gland bulbourethralis not visible because it was covered by muscles and glands in the radix penis is attached to the anales gland. Accessory gland of the male reproductive pangolin consists vesikularis glands, prostate and bulbourethralis glands (Cowper's) play an important role in the reproduction process that is vesicularis and prostate gland as a producer of seminal plasma, whereas bulbourethralis glands act as a producer of seminal plasma as well as rinse of urethra channel.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

Structure, histochemistry and ultrastructure of the male reproductive accessory glands in the neotropical flat-faced fruit-eating bat Artibeus planirostris (Chiroptera: Phyllostomidae)

2013 ◽  
Vol 25 (3) ◽  
pp. 558 ◽  
Author(s):  
Cíntia C. I. Puga ◽  
Mateus R. Beguelini ◽  
Ana C. Negrin ◽  
Caroline M. Christante ◽  
Eliana Morielle-Versute ◽  
...  
Keyword(s):  
Reproductive Strategies ◽  
Periodic Acid ◽  
Prostate Gland ◽  
Basal Cells ◽  
Dorsal Region ◽  
Ventral Region ◽  
Periodic Acid Schiff ◽  
Accessory Glands ◽  
Pseudostratified Epithelium ◽  
Composition Structure

Chiroptera, the second largest mammalian order, presents different reproductive strategies and unique reproductive features. However, there are few reports regarding male reproductive accessory glands (RAGs) in Chiroptera. Thus, the aim of the present study was to characterise the RAGs of the exclusively neotropical bat Artibeus planirostris (Chiroptera: Phyllostomidae) macroscopically, microscopically and ultrastructurally. The RAGs were composed of a prostatic complex with two regions (ventral and dorsal) and paraurethral and bulbourethral glands, but no seminal vesicles. The ventral region had an undefined epithelium, with secretory and basal cells, and its secretions were periodic acid-Schiff (PAS) positive. The dorsal region received both deferens ducts, had a columnar pseudostratified epithelium with secretory and basal cells. There were two types of secretions from the dorsal region: one that was basophilic and another that was mixed PAS positive and PAS negative. The paraurethral glands were dispersed in the connective tissue of the urethra, whereas the bulbourethral glands were located in the penile root. Histological and ultrastructural data confirmed the prostatic nature of the ventral and dorsal regions and the holocrine nature of the ventral region, with the latter finding never having been described previously for the prostate gland. Our findings demonstrate the wide discrepancy of RAGs between A. planirostris and other mammals in terms of their composition, structure and morphology.

scholarly journals Three-dimensional analysis of seminiferous tubules and spermatogenic waves in mice

Reproduction ◽  
2017 ◽  
Vol 154 (5) ◽  
pp. 569-579 ◽  
Author(s):  
Hiroki Nakata ◽  
Takahiro Sonomura ◽  
Shoichi Iseki
Keyword(s):  
High Performance ◽  
Average Length ◽  
Periodic Acid ◽  
3D Structure ◽  
Three Dimensional ◽  
Rete Testis ◽  
Seminiferous Tubules ◽  
Periodic Acid Schiff ◽  
Adult Mice ◽  
Branching Points

The aim of the present study was to reconstruct seminiferous tubules and analyze spermatogenic waves in seminiferous epithelia in developing and adult mice using serial paraffin sections and high-performance three-dimensional (3D) reconstruction software. By labeling the basement membrane of seminiferous tubules with fluorescent immunohistochemistry or periodic acid-Schiff-hematoxylin staining, all seminiferous tubules were reconstructed in 9 testes from 9 different mice, 3 each at 0, 21 and 90 days (adult) postpartum. The 3D structure of seminiferous tubules, including the number and length of tubules as well as the number of connections with the rete testis, branching points and blind ends, was assessed accurately. Although tubules showed marked variations among individual mice, their overall structure was regular and retained from newborn to adult mice. Some seminiferous tubules contained inner portions running distant from the testis surface. In a representative testis at 21 days, the sites at which spermatids initially occurred were examined by labeling acrosomes and were found to be preferentially distributed in the upper and medial portions of the testis close to the rete testis. In a representative adult testis, 76 complete waves with an average length of 16.9 mm were found and their directions were analyzed. The methods used in the present study will be useful for investigating the structure and function of seminiferous tubules in mice and humans under normal and pathological conditions, such as infertility.

scholarly journals Spatial orientation of glycoproteins in membranes of rat liver rough microsomes. II. Transmembrane disposition and characterization of glycoproteins.

1978 ◽  
Vol 78 (3) ◽  
pp. 894-909 ◽  
Author(s):  
E Rodriguez Boulan ◽  
D D Sabatini ◽  
B N Pereyra ◽  
G Kreibich
Keyword(s):  
Rat Liver ◽  
Concanavalin A ◽  
Gel Electrophoresis ◽  
Periodic Acid ◽  
Sodium Dodecyl ◽  
Microsomal Protein ◽  
Outer Face ◽  
Membrane Glycoproteins ◽  
Periodic Acid Schiff ◽  
Low Concentrations

Rat liver microsomal glycoproteins were purified by affinity chromatography on concanavalin A Sepharose columns from membrane and content fractions, separated from rough microsomes (RM) treated with low concentrations of deoxycholate (DOC). All periodic acid-Schiff (PAS)-positive glycoproteins of RM showed affinity for concanavalin A Sepharose; even after sodium dodecyl sulfate (SDS) acrylamide gel electrophoresis, most of the microsomal glycoproteins bound [125I]concanavalin A added to the gels, as detected by autoradiography. Two distinct sets of glycoproteins are present in the membrane and content fractions derived from RM. SDS acrylamide gel electrophoresis showed that RM membranes contain 15--20 glycoproteins (15--22% of the total microsomal protein) which range in apparent mol wt from 23,000 to 240,000 daltons. A smaller set of glycoproteins (five to seven polypeptides), with apparent mol wt between 60,000 and 200,000 daltons, was present in the microsomal content fraction. The disposition of the membrane glycoproteins with respect to the membrane plane was determined by selective iodination with the lactoperoxidase (LPO) technique. Intact RM were labeled on their outer face with 131I and, after opening of the vesicles with 0.05% DOC, in both faces with 125I. An analysis of iodination ratios for individual proteins separated electrophoretically showed that in most membrane glycoproteins, tyrosine residues are predominantly exposed on the luminal face of the vesicles, which is the same face on which the carbohydrate moieties are exposed. Several membrane glycoproteins are also exposed on the cytoplasmic surface and therefore have a transmembrane disposition. In this study, ribophorins I and II, two integral membrane proteins (mol wt 65,000 and 63,000) characteristic of RM, were found to be transmembrane glycoproteins. It is suggested that the transmembrane disposition of the ribophorins may be related to their possible role in ribosome binding and in the vectorial transfer of nascent polypeptides into the microsomal lumen.

scholarly journals A Glycoproteinaceous Secretion in the Seminal Vesicles of the Termite Coptotermes gestroi (Isoptera: Rhinotermitidae)

Insects ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 428
Author(s):  
Lara T. Laranjo ◽  
Ives Haifig ◽  
Ana Maria Costa-Leonardo
Keyword(s):  
Reproductive System ◽  
Periodic Acid ◽  
First Record ◽  
Distal Portion ◽  
Seminal Vesicles ◽  
Male Reproductive System ◽  
Periodic Acid Schiff ◽  
Accessory Glands ◽  
Primary Reproductives ◽  
Coptotermes Gestroi

Coptotermes gestroi is a subterranean termite with colonies generally headed by a pair of primary reproductives, although neotenics may occur. In this study, the male reproductive system was compared during different life stages of nymphs, alates, neotenic reproductives, and kings of C. gestroi, focusing on the modifications of this system along the maturation of these individuals. The structure of the male reproductive system follows the pattern described for insects, although C. gestroi males do not exhibit conspicuous penises and differentiated accessory glands. In kings, each testis consisted of about seven lobes, significantly increased in size as compared to younger males. The spermatogenesis begins in third-instar nymphs, which already presented spermatozoa in the testes. The seminal vesicles are individualized in C. gestroi and have a secretory distal portion and a proximal portion with a role in spermatozoa storage. The secretion of the seminal vesicles is strongly periodic acid Schiff (PAS)-positive, whereas the xylidine Ponceau test revealed proteins that increase in quantity while the males become older. This is the first record of glycoproteins in the lumen of seminal vesicles in termites. Further studies will clarify how they are produced and interact in the physiology and nutrition of the non-flagellate spermatozoa of C. gestroi.

scholarly journals Surface organization and composition of Euglena. II. Flagellar mastigonemes

1978 ◽  
Vol 77 (3) ◽  
pp. 805-826 ◽  
Author(s):  
GB Bouck ◽  
A Rogalski ◽  
A Valaitis
Keyword(s):  
Unit Length ◽  
Periodic Acid ◽  
Minor Component ◽  
Positive Staining ◽  
Periodic Acid Schiff ◽  
Phenol Extraction ◽  
Low Concentrations ◽  
Flagellar Membrane ◽  
Pentose Sugar ◽  
Layer Chromatography

The surface of the Euglena flagellum is coated with about 30,000 fine filaments of two distinct types. The longer of these nontubular mastigonemes (about 3 micron) appear to be attached to the paraflagellar rod whereas the shorter nontubular mastigonemes (about 1.5 micron) are the centrifugally arranged portions of a larger complex, which consists of an attached unit parallel to and outside of the flagellar membrane. Units are arranged laternally in near registration and longitudinally overlap by one-half of a unit length. Rows of mastigoneme units are firmly attached to the axoneme microtubules or to the paraflagellar rod as evidenced by their persistence after removal of the flagellar membrane with neutral detergents. SDS-acrylamide gels of whole flagella revealed about 30 polypeptides, of which two gave strong positive staining with the periodic acid-Schiff (PAS) procedure. At least one of these two bands (glycoproteins) has been equated with the surface mastigonemes by parallel analysis of isolated and purified mastigonemes, particularly after phenol extraction. The faster moving glycoprotein has been selectively removed from whole flagella and from the mastigoneme fraction with low concentrations of neutral detergents at neutral or high pH. The larger glycoprotein was found to be polydisperse when electrophoresed through 1% agarose/SDS gels. Thin-layer chromatography of hydrolysates of whole flagella or of isolated mastigonemes has indicated that the major carbohydrate moiety is the pentose sugar, xylose, with possibly a small amount of glucose and an unknown minor component.

scholarly journals Isolation and partial characterization of the sialoglycoprotein fraction of murine erythrocyte ghosts.

1982 ◽  
Vol 93 (3) ◽  
pp. 583-590 ◽  
Author(s):  
A H Sarris ◽  
G E Palade
Keyword(s):  
Periodic Acid ◽  
Limited Proteolysis ◽  
Erythrocyte Ghosts ◽  
Periodic Acid Schiff ◽  
Low Concentrations ◽  
Sds Page ◽  
Schiff Reagent ◽  
High Concentrations ◽  
Partition Method

With the lithium diiodosalicylate (LIS1) extraction-phenol partition method, we have isolated a sialoglycoprotein fraction from DBA/2 mouse erythrocyte ghosts. We have demonstrated that the Laemmeli system for SDS PAGE can resolve this fraction into four monomers of which two (gp-2.1 and gp-3.1) appear to be authentic, whereas the other two (gp-2.2 and gp-3.2) are probably generated from gp-2.1 and gp-3.1, by limited proteolysis during the isolation procedure. All four components contain O-acetylated neuraminic acid residues, can be stained with Periodic acid-Schiff reagent (PAS) and with Coomassie Brilliant Blue (CB), and can be radioiodinated with the lactoperoxidase-glucose oxidase (LPO-GO) method. All monomers but especially gp-2.1 and gp-3.1 generate characteristic aggregates during solubilization in SDS. The aggregation is enhanced by boiling at high concentrations, and can be reversed by boiling at low concentrations. In addition, the fraction contains a diffuse component present also in ghosts which stains poorly with CB and with PAS and cannot be radioiodinated by the LPO-GO technique. SDS PAGE in the Steck and Yu gel system does not give an accurate separation of the sialoglycoprotein monomers.

Detection of Carriers in Glanzmann's Thrombasthenia

1983 ◽  
Vol 49 (03) ◽  
pp. 182-186
Author(s):  
G T E Zonneveld ◽  
E F van Leeuwen ◽  
A Sturk ◽  
J W ten Cate
Keyword(s):  
Bleeding Time ◽  
Periodic Acid ◽  
Type I ◽  
Clot Retraction ◽  
Periodic Acid Schiff ◽  
Glanzmann’S Thrombasthenia ◽  
Aggregation Response ◽  
Glanzmann's Thrombasthenia ◽  
Sds Page ◽  
Reduced State

SummaryQuantitative glycoprotein (GP) analysis of whole platelets or platelet membranes was performed by SDS-polyacrylamide gelelectrophoresis (SDS-PAGE) and periodic acid Schiff staining in the families of two unrelated Glanzmann’s thrombasthenia (GT) patients. Each family consisted of two symptom free parents, a symptom free daughter and a GT daughter. All symptom free members had a normal bleeding time, clot retraction and platelet aggregation response to adenosine 5’-diphosphate (ADP), collagen and adrenalin. Platelet Zw* antigen was normally expressed in these subjects. GT patiens, classified as a type I and II subject, showed reduced amounts of GP lib and of GP nia. Analysis of isolated membranes in the non-reduced state, however, showed that the amount of GP Ilia was also reduced in three of the four parents, whereas one parent (of the GT type I patient) and the two unaffected daughters had normal amounts of GP Ilia. Quantitative SDS-PAGE may therefore provide a method for the detection of asymptomatic carriers in GT type I and II.

Swim bladder mycosis in farmed rainbow trout Oncorhynchus mykiss caused by Phoma herbarum and experimental verification of pathogenicity

2020 ◽  
Vol 138 ◽  
pp. 237-246 ◽  
Author(s):  
J Řehulka ◽  
A Kubátová ◽  
V Hubka
Keyword(s):  
Rainbow Trout ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Bladder Wall ◽  
Swim Bladder ◽  
Cell Reactivity ◽  
Periodic Acid Schiff ◽  
Posterior Portion ◽  
Hepatic Congestion ◽  
Phoma Herbarum

In this study, spontaneous swim bladder mycosis was documented in a farmed fingerling rainbow trout from a raceway culture system. At necropsy, the gross lesions included a thickened swim bladder wall, and the posterior portion of the swim bladder was enlarged due to massive hyperplasia of muscle. A microscopic wet mount examination of the swim bladder contents revealed abundant septate hyphae, and histopathological examination showed periodic acid-Schiff-positive mycelia in the lumen and wall of the swim bladder. Histopathological examination of the thickened posterior swim bladder revealed muscle hyperplasia with expansion by inflammatory cells. The causative agent was identified as Phoma herbarum through morphological analysis and DNA sequencing. The disease was reproduced in rainbow trout fingerlings using intraperitoneal injection of a spore suspension. Necropsy in dead and moribund fish revealed extensive congestion and haemorrhages in the serosa of visceral organs and in liver and abdominal serosanguinous fluid. Histopathological examination showed severe hepatic congestion, sinusoidal dilatation, Kupffer cell reactivity, leukostasis and degenerative changes. Fungi were disseminated to the liver, pyloric caeca, kidney, spleen and heart. Although infections caused by Phoma spp. have been repeatedly reported in fish, species identification has been hampered by extensive taxonomic changes. The results of this study confirmed the pathogenicity of P. herbarum in salmonids by using a reliably identified strain during experimental fish infection and provides new knowledge regarding the course of infection.

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