Platelet Storage-granule Defects

2009 ◽  
pp. 469-471
Author(s):  
Shawn M. Jobe
Keyword(s):  
Platelet Storage ◽  
Storage Granule

Loss of Thrombin-Induced Ca2+ Mobilization in a Subpopulation of Platelets during Storage

1991 ◽  
Vol 66 (03) ◽  
pp. 350-354 ◽  
Author(s):  
Rob Fijnheer ◽  
Christa H E Homburg ◽  
Berend Hooibrink ◽  
Martine N Boomgaard ◽  
Dirk de Korte ◽  
...  
Keyword(s):  
Human Platelets ◽  
Flow Cytometer ◽  
Maximal Concentration ◽  
Gradual Decline ◽  
Platelet Storage ◽  
Functional Defect ◽  
Total Fluorescence ◽  
Induced Changes

SummaryThrombin-induced changes in cytosolic free Ca2+ ([Ca2+]i) were studied in human platelets that had been stored for up to 6 days. Changes in [Ca2+]i were measured with Indo-1-loaded platelets and quantitated with two different methods: (i) measurement of the changes in total fluorescence; (ii) measurement of the [Ca2+]i changes in individual platelets in a flow cytometer, allowing the detection of non-responding platelets. The maximal concentration of [Ca2+]i after stimulation with 0.5 U of thrombin/ml decreased from 544 ± 58 nM (mean ± SEM, n = 6) on day 0, to 276 ± 9 nM on day 3 and to 203 ± 23 nM on day 6. The percentage of platelets responding to 0.5 U of thrombin/ml declined from 90 ± 2% on day 0 to 72 ± 4% on day 3, and to 47 ± 8% on day 6. Nevertheless, also the responding platelets showed a decreased rise in [Ca2+]i.The study shows that during platelet storage a decrease in the rise in [Ca2+]i upon thrombin stimulation occurs. This decrease is partly due to the formation of a subpopulation of platelets that is completely unresponsive and partly due to a decreased responsiveness in the remainder of the platelets; it is not due to a gradual decline in [Ca2+]i rise in all platelets. This phenomenon provides new insight in the functional defect of stored platelets.

Correlation Between Noradrenaline Fluxes, Metabolism and Compartmentalisation in Human Platelets

1982 ◽  
Vol 48 (01) ◽  
pp. 062-066 ◽  
Author(s):  
Chantal Legrand ◽  
Véronique Dubernard ◽  
Philippe Meyer
Keyword(s):  
Noradrenaline Release ◽  
Human Platelets ◽  
Release Of Noradrenaline ◽  
Storage Pool ◽  
Platelet Storage ◽  
Efflux Of Noradrenaline ◽  
Preferential Localization

Summary(3H) noradrenaline was taken up by human platelets and partially converted into sulfoconjugated noradrenaline. This uptake was inhibited by drugs which have been previously shown to impair the uptake of 5-HT (ouabain, chlorimipramine) or the storage of 5-HT (tyramine, reserpine) by platelets. In addition, tyramine and reserpine stimulated the formation of sulfoconjugated noradrenaline. The efflux of noradrenaline from platelets was measured in parallel and was found to be directly related to the proportion of non metabolized to metabolized noradrenaline in the cells. Unlike tyramine, which induced a similar release of noradrenaline and 5-HT, reserpine was less effective at inducing noradrenaline release than 5-HT release. This study indicates a preferential localization of noradrenaline in the granular pool of human platelets with the existence of an extragranular sulfoconjugated pool which is increased when the granular storage of noradrenaline is impaired. Studies of noradrenaline fluxes and metabolism may be useful in the understanding of both acquired and inherited platelet storage pool defects.

Detection of an Acquired Platelet Storage Pool Disease in Three Patients with a Myeloproliferative Disorder

1979 ◽  
Vol 42 (02) ◽  
pp. 794-796 ◽  
Author(s):  
Francine Rendu ◽  
Marilyne Lebret ◽  
Alan Nurden ◽  
Jacques P Caen
Keyword(s):  
Myeloproliferative Disorder ◽  
Storage Pool ◽  
Platelet Storage ◽  
Storage Pool Disease

scholarly journals Platelet storage and functional integrity

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Gianmatteo Vit ◽  
Harald Klüter ◽  
Patrick Wuchter
Keyword(s):  
Platelet Transfusion ◽  
Structural Characteristics ◽  
Storage Conditions ◽  
Transfusion Practice ◽  
Common Interest ◽  
Laboratory Staff ◽  
German Medical Association ◽  
Platelet Storage ◽  
Technological Advances ◽  
Assessment Procedures

AbstractPlatelet transfusion is a topic of common interest for many specialists involved in patient care, from laboratory staff to clinical physicians. Various aspects make this type of transfusion different from those of other blood components. In this review, the challenges in platelet transfusion practice that are relevant for laboratory colleagues will be discussed, highlighting how the biochemical and structural characteristics of these blood elements directly affect their function and consequently the clinical outcome. More than 1,300 platelet concentrates are transfused in Germany every day, and several types are offered by their respective manufacturers. We describe the technological advances in platelet concentrate production, with a focus on how the storage conditions of platelets can be improved. Laboratory quality assessment procedures for a safe transfusion are discussed in detail. For this purpose, we will refer to the Hemotherapy Directives (Richtlinie Hämotherapie) of the German Medical Association.

The relationship between the duration of platelet storage and the development of transfusion reactions

Transfusion ◽  
1998 ◽  
Vol 38 (3) ◽  
pp. 229-235 ◽  
Author(s):  
CB Sarkodee-Adoo ◽  
JM Kendall ◽  
R Sridhara ◽  
EJ Lee ◽  
CA Schiffer
Keyword(s):  
Platelet Storage ◽  
The Relationship ◽  
Transfusion Reactions

scholarly journals A Platelet Acquired Storage Pool Disorder Associated with Tamoxifen Therapy

2012 ◽  
Vol 2012 ◽  
pp. 1-3 ◽  
Author(s):  
Lalitha Nayak ◽  
Alvin H. Schmaier
Keyword(s):  
Breast Cancer ◽  
Platelet Function ◽  
Tooth Extraction ◽  
Function Analysis ◽  
Tamoxifen Therapy ◽  
Storage Pool ◽  
Prolonged Bleeding ◽  
Platelet Storage

The antiestrogenic drug tamoxifen, used in patients with breast cancer, is associated with an increase in arterial and venous thrombotic events, the mechanism of which is not clearly understood. We report a case of a lady who presented with new bruising and prolonged bleeding following a tooth extraction 4–6 weeks after starting tamoxifen. Investigations were consistent with an acquired platelet storage pool disorder. Repeat platelet function analysis was normal, performed 3 months after discontinuation of tamoxifen. We present a previously clinically unreported effect of tamoxifen on platelet function.

scholarly journals Platelets release mitochondria serving as substrate for bactericidal group IIA-secreted phospholipase A2 to promote inflammation

Blood ◽  
2014 ◽  
Vol 124 (14) ◽  
pp. 2173-2183 ◽  
Author(s):  
Luc H. Boudreau ◽  
Anne-Claire Duchez ◽  
Nathalie Cloutier ◽  
Denis Soulet ◽  
Nicolas Martin ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Rickettsia Prowazekii ◽  
Platelet Storage ◽  
Secreted Phospholipase A2 ◽  
Molecular Pattern ◽  
Key Points

Key Points When activated and in platelet storage bags, platelets release respiratory-competent mitochondria, a recognized damage-associated molecular pattern. Mitochondria, descendant of Rickettsia prowazekii, serve as substrate for bactericidal sPLA2-IIA to promote inflammation.

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