Collection of Interstitial Fluid and Seminiferous Tubule Fluid from the Rat Testis

1993 ◽  
pp. 265-273 ◽  
Author(s):  
Georgia L. Rehnberg
Keyword(s):  
Seminiferous Tubule ◽  
Interstitial Fluid ◽  
Rat Testis ◽  
Tubule Fluid

The effect of germ cell complement on the presence of oxytocin in the interstitial and seminiferous tubule fluid of the rat testis

1994 ◽  
Vol 143 (3) ◽  
pp. 471-478 ◽  
Author(s):  
H D Nicholson ◽  
H M Greenfield ◽  
J Frayne
Keyword(s):  
Heat Treatment ◽  
Germ Cell ◽  
Germ Cells ◽  
Seminiferous Tubule ◽  
Leydig Cells ◽  
Male Rats ◽  
Rat Testis ◽  
Pachytene Spermatocytes ◽  
Tubule Fluid

Abstract In the rat testis oxytocin has been localized to the Leydig cells, and these cells have been shown to produce oxytocin in vitro. The present study was performed to determine whether oxytocin is present in the interstitial fluid (IF) and seminiferous tubule fluid (TF) of the rat and whether concentrations of the peptide vary within the two compartments following germ cell destruction. In order to destroy germ cells adult male rats were anaesthetized and their scrotal regions placed in a water bath at 43 °C for 20 min. Control animals were subjected to anaesthesia alone. Groups of 6 animals were killed 3, 7 and 21 days after heat treatment and their testes removed for histological examination or fluid extraction. IF and TF were separated and the oxytocin content of the fluids measured by radioimmunoassay. Immunoreactive oxytocin was detected in both the IF (100 ±11 pg/ml) and TF (27± 4 pg/ml) of control rats and this immunoreactivity co-eluted with the authentic peptide following HPLC. Three days after heat treatment IF levels of oxytocin were significantly reduced but TF levels of the peptide were significantly increased. These changes were associated with a lack of pachytene spermatocytes in the histological sections. Seven and 21 days after heat treatment the levels of oxytocin in the IF and TF were not significantly different from control levels. Similar changes in IF and TF oxytocin levels were seen in a second experiment when pachytene spermatocytes were removed using the testicular toxicant methoxyacetic acid. To investigate whether oxytocin passes from the IF into the TF, 3H-oxytocin was infused into the testicular arteries of both control and heat-treated rats and the rats killed at regular intervals over the next 24 h. In both groups of animals <5% of the 3H-oxytocin passed from the IF into the TF. These data suggest that the seminiferous tubule as well as the Leydig cells may secrete oxytocin and that this secretion may be influenced by the presence of germ cells, particularly pachytene spermatocytes. Journal of Endocrinology (1994) 143, 471–478

Effects of hypophysectomy and subsequent FSH and testosterone treatment on inhibin production by adult rat testes

1985 ◽  
Vol 105 (1) ◽  
pp. 1-6 ◽  
Author(s):  
C. L. Au ◽  
D. M. Robertson ◽  
D. M. de Kretser
Keyword(s):  
Seminiferous Tubule ◽  
Hormonal Control ◽  
Human Chorionic Gonadotrophin ◽  
Experimental Conditions ◽  
Adult Rats ◽  
Adult Rat ◽  
Fluid Production ◽  
Tubule Fluid

ABSTRACT The hormonal control of inhibin production by adult rat testes was investigated using an in-vitro inhibin bioassay validated for the measurement of inhibin activity in charcoal-treated rat testicular extracts. The effect of hypophysectomy examined at 16 h, 3, 7 and 42 days after surgery showed a decrease in testicular inhibin content and seminiferous tubule fluid production by 7 days and a decrease in inhibin production by 42 days. Serum FSH and LH were suppressed 3 days after surgery. In 30-day chronically hypophysectomized adult rats treated for 3 days with twice daily s.c. injections of (a) human FSH (hFSH, 22 i.u./rat per day), (b) testosterone (5 mg/rat per day), (c) hFSH + testosterone (same doses as a and b), or (d) human chorionic gonadotrophin (hCG, 12 i.u./rat per day), hFSH or hFSH and testosterone stimulated an increase in testicular inhibin content but not in inhibin production or tubule fluid production. Testosterone and hCG had no effect on these parameters. It is concluded that in vivo, FSH alone stimulates an increase in testicular inhibin content. The failure to observe an increase in inhibin production in vivo is attributed to the suppression of seminiferous tubule fluid production under the same experimental conditions. J. Endocr. (1985) 105, 1–6

scholarly journals The levels and possible involvement of leukotriene B4and prostaglandin F2αin the control of interstitial fluid volume in the rat testis

1990 ◽  
Vol 13 (5) ◽  
pp. 408-418 ◽  
Author(s):  
D. R. E. ABAYASEKARA ◽  
L. O. KURLAK ◽  
J. Y. JEREMY ◽  
P. DANDONA ◽  
R. M. SHARPE ◽  
...  
Keyword(s):  
Interstitial Fluid ◽  
Fluid Volume ◽  
Rat Testis ◽  
Interstitial Fluid Volume

Interstitial fluid volume in the rat testis: androgen-dependent regulation by the seminiferous tubules?

1989 ◽  
Vol 120 (2) ◽  
pp. 215-NP ◽  
Author(s):  
S. Maddocks ◽  
R. M. Sharpe
Keyword(s):  
Leydig Cells ◽  
Interstitial Fluid ◽  
Single Injection ◽  
Fluid Volume ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Rat Testis ◽  
Adult Rat ◽  
Interstitial Fluid Volume ◽  
Dependent Mechanism

ABSTRACT Regulation of testicular interstitial fluid (IF) volume has been investigated in adult male rats in which the Leydig cells were selectively destroyed with a single i.p. injection of ethane dimethane sulphonate (EDS). Following this treatment, some animals also received testosterone supplementation by s.c. injection every 3 days, beginning either from the time of EDS injection, or 3–12 days afterwards. The volume of IF obtained by drip collection was determined, and testosterone and gonadotrophin concentrations measured in blood and in IF. Testosterone levels in IF and serum became undetectable by 3 days after EDS treatment. IF volume was reduced by 50% (P < 0·01) to reach a minimum level between 6 and 9 days after treatment. However, this decline was prevented in the absence of Leydig cells by supplementation with testosterone from the time of EDS injection, a treatment which also kept gonadotrophins at minimum or undetectable levels. Furthermore, the reduced IF volume seen up to 9 days after treatment with EDS alone could be restored to control levels within 3 days by a single injection of testosterone. The results obtained demonstrate that androgens, but not Leydig cells or gonadotrophins, are required for the maintenance of interstitial fluid volume in the adult rat testis. It is suggested that the seminiferous tubules may mediate this response, through an androgen-dependent mechanism. Journal of Endocrinology (1989) 120, 215–222

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