Functional microbial characteristics in acidogenic fermenters of organic wastes for production of volatile fatty acids

This study aimed to investigate the production of polyhydroxyalkanoates (PHAs), a biodegradable polymer from organic wastes by Pseudomonas oleovorans. Volatile fatty acids (VFAs) from acidogenic fermentations of chicken manure (VFAs-CM) and potato peels (VFAs-PP), rich in organic matter majorly acetic (49.9%), butyric (15%) and propionic acids (11.1%) were utilized as substrates for microbial processes. During 72 h of cultivations, samples were withdrawn at intervals and analyzed for cell growth parameters, PHAs accumulation and polymeric properties. The highest biopolymer accumulation (0.39 g PHAs/g DCW) was achieved at 48 h of cultivation from medium containing VFAs-PP as the sole source of carbon. On characterization, the produced biopolymers were shown to be semi-crystalline of carbonyl C=O group. Additionally, thermogravimetric analysis (TGA) showed that the produced biopolymers demonstrated the capability to withstand thermal degradation above prescribed temperatures at which cross-linking isomerization reaction occurs, which is a vital property denoting the thermal stability of biopolymer.

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Recovery of mixed volatile fatty acids from anaerobically fermented organic wastes by vapor permeation membrane contactors

Bioresource Technology ◽

10.1016/j.biortech.2017.11.061 ◽

2018 ◽

Vol 250 ◽

pp. 548-555 ◽

Cited By ~ 21

Author(s):

Senem Aydin ◽

Hatice Yesil ◽

A. Evren Tugtas

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Organic Wastes ◽

Vapor Permeation ◽

Membrane Contactors

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Acidogenic fermentation of organic wastes for production of volatile fatty acids

10.1016/b978-0-323-90633-3.00005-5 ◽

2022 ◽

pp. 343-366

Author(s):

Le Zhang ◽

To-Hung Tsui ◽

Kai-Chee Loh ◽

Yanjun Dai ◽

Yen Wah Tong

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Organic Wastes ◽

Acidogenic Fermentation

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Study of two-phase anaerobic digestion of corn stover: Focusing on the conversion of volatile fatty acids and microbial characteristics in UASB reactor

Industrial Crops and Products ◽

10.1016/j.indcrop.2020.113097 ◽

2020 ◽

pp. 113097

Author(s):

Yan Li ◽

Haipeng Xu ◽

Xiaolu Yi ◽

Yuxiao Zhao ◽

Fuqiang Jin ◽

...

Keyword(s):

Fatty Acids ◽

Anaerobic Digestion ◽

Corn Stover ◽

Volatile Fatty Acids ◽

Uasb Reactor ◽

Two Phase ◽

Microbial Characteristics

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Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

HKIE Transactions ◽

10.33430/v26n2thie-2018-0041 ◽

2019 ◽

Vol 26 (2) ◽

pp. 63-71

Author(s):

Ling Leng ◽

Ying Wang ◽

Peixian Yang ◽

Takashi Narihiro ◽

Masaru Konishi Nobu ◽

...

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Microbial Consortia ◽

Chain Elongation ◽

Desulfovibrio Vulgaris ◽

Rrna Gene ◽

Selective Enrichment ◽

Microbial Network ◽

Cost Efficient ◽

Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

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МЕТАБОЛИТЫ ПРОБИОТИЧЕСКИХ БАКТЕРИЙ ОТВЕЧАЮТ ЗА ЭФФЕКТИВНОСТЬ ДЕЙСТВИЯ ПРОБИОТИКА "ПРОФОРТ®"

10.33845/0033-3239-2019-68-9-10-45-47 ◽

2019 ◽

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Broiler Chicks ◽

Bacillus Sp

МЕЛИКИДИ В.Х., ТЮРИНА Д.Г., СЕЛИВАНОВ Д.Г., НОВИКОВА Н.И. ООО «БИОТРОФ», Санкт-Петербург Аннотация: Приведены данные исследования методом газожидкостной хроматомасс-спектрометрии метаболитов, синтезируемых пробиотическими бактериями, входящими в состав кормовой добавки «Профорт®». Проведен опыт в условиях интенсивного промышленного птицеводства по применению кормовой добавки «Профорт®» (50 тыс. голов бройлеров в группе). Среди метаболитов пробиотических штаммов Enterococcus sp. и Bacillus sp. обнаружены такие полезные вещества, как молочная кислота, уксусная, пропионовая и другие короткоцепочечные (летучие) жирные кислоты, активные пептиды. Результаты зоотехнического опыта показали, что при скармливании бройлерам пробиотика «Профорт®» (500 г/т) живая масса при убое в 40 дней была выше контроля на 6,9%, конверсия корма улучшилась на 3,0%, а европейский индекс продуктивности бройлеров - на 5,69%. Ключевые слова: ПРОБИОТИКИ, МЕТАБОЛИТЫ, ЛЕТУЧИЕ ЖИРНЫЕ КИСЛОТЫ, ЦЫПЛЯТА-БРОЙЛЕРЫ,ПРОДУКТИВНОСТЬ, PROBIOTICS, METABOLITES, VOLATILE FATTY ACIDS, BROILER CHICKS,PRODUCTIVITY

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