Validation of bioanalytical chromatographic methods for the quantification of drugs in biological fluids

Author(s):  
Oskar González ◽  
Rosa María Alonso
1961 ◽  
Vol 39 (2) ◽  
pp. 439-451 ◽  
Author(s):  
C. K. Harris ◽  
E. Tigane ◽  
C. S. Hanes

A method based on the use of miniature ion-exchange columns has been devised for isolating small amounts of amino acids from biological fluids and tissue extracts. The amino acids are isolated virtually free from proteins, sugars, and inorganic cations in a single treatment.Techniques are described for the preparation of solutions of the isolated amino acids for quantitative analysis by paper chromatography.


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