RENAL EPITHELIAL CELLS IN CULTURE: A COMPARATIVE STUDY OF THE FUNCTIONAL ACTIVITIES OF RABBIT KIDNEY PROXIMAL TUBULE CELLS IN PRIMARY CULTURES AND THE LLC-PK1 AND OK CELLS

1992 ◽  
pp. 713-715
Author(s):  
J P MORIN ◽  
C MONTEIL ◽  
J P FILLASTRE
Keyword(s):  
Comparative Study ◽  
Primary Cultures ◽  
Rabbit Kidney ◽  
Proximal Tubule Cells ◽  
Renal Epithelial Cells ◽  
Functional Activities ◽  
Ok Cells ◽  
Kidney Proximal Tubule ◽  
Cells In Culture ◽  
Tubule Cells

Polarity and transport properties of rabbit kidney proximal tubule cells on collagen IV-coated porous membranes

1995 ◽  
Vol 269 (1) ◽  
pp. F22-F30 ◽  
Author(s):  
I. Genestie ◽  
J. P. Morin ◽  
B. Vannier ◽  
G. Lorenzon
Keyword(s):  
Proximal Tubule ◽  
Transport Properties ◽  
Collagen Iv ◽  
Transport Processes ◽  
Rabbit Kidney ◽  
Porous Membranes ◽  
Proximal Tubule Cells ◽  
Kidney Proximal Tubule ◽  
Apical Compartment ◽  
Tubule Cells

A high degree of functional polarity has been obtained in primary cultures of rabbit kidney proximal tubule cells grown on collagen IV-coated porous membranes. Tight confluency was attained 6 days after seeding and maintained for at least 6 more days, as shown by analysis of paracellular inulin diffusion. From day 6 onward, L-lactate, ammonia, and D-glucose concentration gradient and a pH difference of approximately 1 unit developed between the two nutrient medium compartments. Confluent monolayers expressed organic ion transport properties higher than those formerly reported for other cell models. Transcellular transport of 20 microM tetraethylammonium was directed from basal to apical compartment and was specifically inhibited by mepiperphenidol (1 mM). Unidirectional transport of 2.4 microM p-aminohippurate also occurred from basal to apical compartment, was saturable, and specifically inhibited by probenecid (1 mM). These results suggest that rabbit kidney proximal tubule cells, cultured under the experimental conditions described here, may be a useful model for the in vitro study of highly polarized renal transport processes.

Changes in expression and subcellular localization of annexin IV in rabbit kidney proximal tubule cells during primary culture

1995 ◽  
Vol 165 (2) ◽  
pp. 313-322 ◽  
Author(s):  
D. Massey-Harroche ◽  
V. Traverso ◽  
N. Mayran ◽  
V. Francou ◽  
A. Vandewalle ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Proximal Tubule ◽  
Primary Culture ◽  
Rabbit Kidney ◽  
Proximal Tubule Cells ◽  
Kidney Proximal Tubule ◽  
Tubule Cells ◽  
Annexin Iv
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