Self-assembled nanostructures for bone tissue engineering

Cranial, maxillofacial, and oral fractures, as well as large bone defects, are currently being treated by auto- and allograft procedures. These techniques have limitations such as immune response, donor-site morbidity, and lack of availability. Therefore, the interest in tissue engineering applications as replacement for bone graft has been growing rapidly. Typical bone tissue engineering models require a cell-supporting scaffold in order to maintain a 3-dimensional substrate mimicking in vivo extracellular matrix for cells to attach, proliferate and function during the formation of bone tissue. Combining the understanding of molecular and structural biology with materials engineering and design will enable new strategies for developing biological tissue constructs with clinical relevance. Self-assembled biomimetic scaffolds are especially suitable as they provide spatial and temporal regulation. Specifically, self-assembling peptides capable of in situ gelation serve as attractive candidates for minimally invasive injectable therapies in bone tissue engineering applications.

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A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering

ACS Applied Materials & Interfaces ◽

10.1021/acsami.8b19094 ◽

2019 ◽

Vol 11 (9) ◽

pp. 8749-8762 ◽

Cited By ~ 22

Author(s):

Chen Zhao ◽

Nader Taheri Qazvini ◽

Monirosadat Sadati ◽

Zongyue Zeng ◽

Shifeng Huang ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Hydrogel Scaffold ◽

Hybrid Hydrogel ◽

Self Assembled

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Self-Assembled β-Sheet Architectures for Bone-Tissue Engineering

MRS Proceedings ◽

10.1557/proc-599-305 ◽

1999 ◽

Vol 599 ◽

Cited By ~ 1

Author(s):

G. Spreitzer ◽

J. Doctor ◽

D. W. Wright

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Critical Role ◽

Three Dimensional ◽

Structural Motif ◽

Synthetic Approach ◽

Self Assembled ◽

Β Sheet

AbstractAdvances in the understanding of biomineralization processes in a variety of organisms have revealed the critical role of three-dimensional scaffolding architectures to create a highly functionalized surface. These complex matrices function on a variety of length scales ranging from the macromolecular (10–100 nm) to the cellular (1–10mm) and larger. One dominant structural motif found in many of these architectures is macromolecules containing antiparallel β-pleated sheets. These “hints” from Nature have lead to the iterative design and development of a novel multipurpose platform technology based on a self-assembled periodic peptide architecture for use in bone-tissue engineering. Combining molecular modeling, structural biochemistry and synthetic techniques, we have produced a β-sheet hollow tube peptide nanoassembly. Such a synthetic approach allows for the template's designed parameters of electrostatic, geometric and stereochemical complimentarily to match those of the desired biomineral. Consequently, these templates readily nucleate calcite. Future studies will investigate the in vitro osteoconductive and osteogenic properties of these templates.

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