Single-molecule resolution of G protein-coupled receptor (GPCR) complexes

2016 ◽  
pp. 55-72 ◽  
Author(s):  
Kim C. Jonas ◽  
Ilpo Huhtaniemi ◽  
Aylin C. Hanyaloglu
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Fluorophore Labeling, Nanodisc Reconstitution and Single-molecule Observation of a G Protein-coupled Receptor

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (12) ◽  
Author(s):  
Rajan Lamichhane ◽  
Jeffrey Liu ◽  
Raymond Pauszek III ◽  
David Millar
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles

Nanoscale ◽  
2020 ◽  
Vol 12 (21) ◽  
pp. 11518-11525 ◽  
Author(s):  
Rachael L. Grime ◽  
Joelle Goulding ◽  
Romez Uddin ◽  
Leigh A. Stoddart ◽  
Stephen J. Hill ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
Single Molecule ◽  
Maleic Acid ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
G Protein Coupled Receptor ◽  
Fluorescence Correlation ◽  
Lipid Particles ◽  
G Protein Coupled

Combining the technologies of encapsulation of GPCRs in SMA lipid particles with fluorescence correlation spectroscopy provides a versatile characterisation platform.

Kinetics of the Initial Steps of G Protein-Coupled Receptor-Mediated Cellular Signaling Revealed by Single-Molecule Imaging

ChemPhysChem ◽  
2005 ◽  
Vol 6 (8) ◽  
pp. 1633-1640 ◽  
Author(s):  
Yoriko Lill ◽  
Karen L. Martinez ◽  
Markus A. Lill ◽  
Bruno H. Meyer ◽  
Horst Vogel ◽  
...  
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
Cellular Signaling ◽  
Single Molecule Imaging ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled ◽  
Kinetics Of

Evidence of G-protein-coupled receptor and substrate transporter heteromerization at a single molecule level

2017 ◽  
Vol 75 (12) ◽  
pp. 2227-2239 ◽  
Author(s):  
Jana Fischer ◽  
Gunnar Kleinau ◽  
Claudia Rutz ◽  
Denise Zwanziger ◽  
Noushafarin Khajavi ◽  
...  
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
G Protein Coupled Receptor ◽  
Single Molecule Level ◽  
G Protein Coupled

scholarly journals Rapid Hop Diffusion of a G-Protein-Coupled Receptor in the Plasma Membrane as Revealed by Single-Molecule Techniques

2005 ◽  
Vol 88 (5) ◽  
pp. 3659-3680 ◽  
Author(s):  
Kenichi Suzuki ◽  
Ken Ritchie ◽  
Eriko Kajikawa ◽  
Takahiro Fujiwara ◽  
Akihiro Kusumi
Keyword(s):  
Plasma Membrane ◽  
G Protein ◽  
Single Molecule ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals The Role of G-Protein-Coupled Receptor Activation by Conformational Selection as Revealed by Single-Molecule Fluorescence

2017 ◽  
Vol 112 (3) ◽  
pp. 327a-328a
Author(s):  
Dennis D. Fernandes ◽  
Libin Ye ◽  
Yuchong Li ◽  
Zhenfu Zhang ◽  
Gregory-Neal Gomes ◽  
...  
Keyword(s):  
G Protein ◽  
Single Molecule ◽  
Receptor Activation ◽  
G Protein Coupled Receptor ◽  
Single Molecule Fluorescence ◽  
Conformational Selection ◽  
G Protein Coupled
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