scholarly journals Content-aware image restoration for electron microscopy

2019 ◽  
pp. 277-289 ◽  
Author(s):  
Tim-Oliver Buchholz ◽  
Alexander Krull ◽  
Réza Shahidi ◽  
Gaia Pigino ◽  
Gáspár Jékely ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Image Restoration ◽  
Content Aware

scholarly journals A “Human-in-the-Loop” Approach for Semi-automated Image Restoration in Electron Microscopy

2019 ◽  
Author(s):  
Joris Roels ◽  
Frank Vernaillen ◽  
Anna Kremer ◽  
Amanda Gonçalves ◽  
Jan Aelterman ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Image Restoration ◽  
Large Scale ◽  
Human In The Loop ◽  
Manual Analysis ◽  
Dataset Size ◽  
Expert Microscopy ◽  
Restoration Algorithms ◽  
User Friendly ◽  
Interactive Graphical User Interface

ABSTRACTThe recent advent of 3D in Electron Microscopy (EM) has allowed for detection of detailed sub-cellular nanometer resolution structures. While being a scientific breakthrough, this has also caused an explosion in dataset size, necessitating the development of automated workflows. Automated workflows typically benefit reproducibility and throughput compared to manual analysis. The risk of automation is that it ignores the expertise of the microscopy user that comes with manual analysis. To mitigate this risk, this paper presents a hybrid paradigm. We propose a ‘human-in-the-loop’ (HITL) approach that combines expert microscopy knowledge with the power of large-scale parallel computing to improve EM image quality through advanced image restoration algorithms. An interactive graphical user interface, publicly available as an ImageJ plugin, was developed to allow biologists to use our framework in an intuitive and user-friendly fashion. We show that this plugin improves visualization of EM ultrastructure and subsequent (semi-)automated segmentation and image analysis.

scholarly journals Content-aware image restoration: pushing the limits of fluorescence microscopy

2018 ◽  
Vol 15 (12) ◽  
pp. 1090-1097 ◽  
Author(s):  
Martin Weigert ◽  
Uwe Schmidt ◽  
Tobias Boothe ◽  
Andreas Müller ◽  
Alexandr Dibrov ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Image Restoration ◽  
Content Aware

An overview of state‐of‐the‐art image restoration in electron microscopy

2018 ◽  
Vol 271 (3) ◽  
pp. 239-254 ◽  
Author(s):  
J. ROELS ◽  
J. AELTERMAN ◽  
H.Q. LUONG ◽  
S. LIPPENS ◽  
A. PIŽURICA ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Image Restoration ◽  
State Of The Art

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

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