Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR

2017 ◽  
pp. 203-242 ◽  
Author(s):  
Christos Pliotas
Keyword(s):  
Ion Channel ◽  
Spin Labeling ◽  
Pulsed Epr ◽  
Site Directed Spin Labeling

scholarly journals Actin-binding cleft closure in myosin II probed by site-directed spin labeling and pulsed EPR

2008 ◽  
Vol 105 (35) ◽  
pp. 12867-12872 ◽  
Author(s):  
J. C. Klein ◽  
A. R. Burr ◽  
B. Svensson ◽  
D. J. Kennedy ◽  
J. Allingham ◽  
...  
Keyword(s):  
Myosin Ii ◽  
Spin Labeling ◽  
Actin Binding ◽  
Pulsed Epr ◽  
Cleft Closure ◽  
Binding Cleft ◽  
Site Directed Spin Labeling

scholarly journals Accessing the distance range of interest in biomolecules: Site-directed spin labeling and DEER spectroscopy

2010 ◽  
Vol 24 (3-4) ◽  
pp. 283-288 ◽  
Author(s):  
Sabine Böhme ◽  
Heinz-Jürgen Steinhoff ◽  
Johann P. Klare
Keyword(s):  
Conformational Changes ◽  
Protein Interactions ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Topological Information ◽  
Pulsed Epr ◽  
Site Directed Spin Labeling ◽  
Intermolecular Distances ◽  
And Function ◽  
Electron Paramagnetic

Investigations on the structure and function of biomolecules often depend on the availability of topological information to build up structural models or to characterize conformational changes during function. Electron paramagnetic resonance (EPR) spectroscopy in combination with site–directed spin labeling (SDSL) allow to determine intra- and intermolecular distances in the range from 4–70 Å, covering the range of interest for biomolecules. The approach does not require crystalline samples and is well suited also for molecules exhibiting intrinsic flexibility. This article is intended to give an overview on pulsed EPR in conjunction with SDSL to study protein interactions as well as conformational changes, exemplified on the tRNA modifying enzyme MnmE.

scholarly journals Site-Directed Spin Labeling Reveals Pentameric Ligand-Gated Ion Channel Gating Motions

2012 ◽  
Vol 102 (3) ◽  
pp. 418a
Author(s):  
Cosma D. Dellisanti ◽  
James M. Raspanti ◽  
Valerie A. Grant ◽  
Susan M. Hanson ◽  
Borna Ghosh ◽  
...  
Keyword(s):  
Ion Channel ◽  
Channel Gating ◽  
Spin Labeling ◽  
Ion Channel Gating ◽  
Site Directed Spin Labeling

scholarly journals Site-Directed Spin Labeling Reveals Pentameric Ligand-Gated Ion Channel Gating Motions

PLoS Biology ◽  
2013 ◽  
Vol 11 (11) ◽  
pp. e1001714 ◽  
Author(s):  
Cosma D. Dellisanti ◽  
Borna Ghosh ◽  
Susan M. Hanson ◽  
James M. Raspanti ◽  
Valerie A. Grant ◽  
...  
Keyword(s):  
Ion Channel ◽  
Channel Gating ◽  
Spin Labeling ◽  
Ion Channel Gating ◽  
Site Directed Spin Labeling

scholarly journals Correction: Braun, T., et al. Expanding the Genetic Code for Site-Directed Spin-Labeling. Int. J. Mol. Sci. 2019, 20, 373

2021 ◽  
Vol 22 (4) ◽  
pp. 1574
Author(s):  
Theresa Braun ◽  
Malte Drescher ◽  
Daniel Summerer
Keyword(s):  
Genetic Code ◽  
Spin Labeling ◽  
Site Directed Spin Labeling

The authors wish to make the following two corrections to this paper [...]

Nucleotide-dependent displacement and dynamics of the α-1 helix in kinesin revealed by site-directed spin labeling EPR

2014 ◽  
Vol 443 (3) ◽  
pp. 911-916 ◽  
Author(s):  
Satoshi Yasuda ◽  
Takanori Yanagi ◽  
Masafumi D. Yamada ◽  
Shoji Ueki ◽  
Shinsaku Maruta ◽  
...  
Keyword(s):  
Spin Labeling ◽  
Site Directed Spin Labeling

scholarly journals Membrane Docking Geometry of GRP1 PH Domain Bound to a Target Lipid Bilayer: An EPR Site-Directed Spin-Labeling and Relaxation Study

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33640 ◽  
Author(s):  
Huai-Chun Chen ◽  
Brian P. Ziemba ◽  
Kyle E. Landgraf ◽  
John A. Corbin ◽  
Joseph J. Falke
Keyword(s):  
Lipid Bilayer ◽  
Spin Labeling ◽  
Ph Domain ◽  
Relaxation Study ◽  
Site Directed Spin Labeling
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