Development of a peptide nucleic acid polymerase chain reaction clamping assay for semiquantitative evaluation of genetically modified organism content in food

2005 ◽  
Vol 344 (2) ◽  
pp. 174-182 ◽  
Author(s):  
C. Peano ◽  
F. Lesignoli ◽  
M. Gulli ◽  
R. Corradini ◽  
M.C. Samson ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Genetically Modified ◽  
Genetically Modified Organism ◽  
Chain Reaction ◽  
Semiquantitative Evaluation ◽  
Polymerase Chain ◽  
Nucleic Acid Polymerase

Application of Magnetic/Magnetic Fluorescent Microsphere Preparation in Genes Food Testing

2014 ◽  
Vol 644-650 ◽  
pp. 5356-5359
Author(s):  
Yong Hui Liu
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Extraction Method ◽  
Genetically Modified ◽  
High Price ◽  
Ammonium Bromide ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Chain Reaction ◽  
Polymerase Chain

Polymerase chain reaction (PCR) and fluorescence quantitative polymerase chain reaction (qRT - PCR) is a major means of detection of genetically modified food, whose key is to extract high quality nucleic acid in quick and high flux. The main nucleic acid extraction method is hexadecyl trimethyl ammonium bromide (CTAB) and sodium dodecyl sulfate (SDS) and kits. Because operation of CTAB and SDS method are cumbersome, require the use of organic solvent and centrifugal unceasingly, thus can not meet the requirement of the rapid development of high throughput; and because of the high price of kit method, its application in the daily inspection is also limited. At the same time, the development trend of genetically modified detection based on nucleic acid level is multiple tests; the key is the preparation of different kinds of fluorescent microspheres and specific probe of microspheres. To solve above problems, this paper put forward nucleic acid extraction method on the basis of the magnetic separation technology.

Investigation on Natural Diets of Larval Marine Animals Using Peptide Nucleic Acid-Directed Polymerase Chain Reaction Clamping

2010 ◽  
Vol 13 (2) ◽  
pp. 305-313 ◽  
Author(s):  
Seinen Chow ◽  
Sayaka Suzuki ◽  
Tadashi Matsunaga ◽  
Shane Lavery ◽  
Andrew Jeffs ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Chain Reaction ◽  
Marine Animals ◽  
Polymerase Chain

A peptide nucleic acid (PNA)-mediated polymerase chain reaction clamping allows the selective inhibition of the ERVWE1 gene amplification

2014 ◽  
Vol 28 (5-6) ◽  
pp. 237-241 ◽  
Author(s):  
Grzegorz Machnik ◽  
Krzysztof Łabuzek ◽  
Estera Skudrzyk ◽  
Piotr Rekowski ◽  
Jarosław Ruczyński ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Gene Amplification ◽  
Peptide Nucleic Acid ◽  
Selective Inhibition ◽  
Chain Reaction ◽  
Polymerase Chain
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