scholarly journals Application of 13C stable isotope labeling liquid chromatography–multiple reaction monitoring–tandem mass spectrometry method for determining intact absorption of bioactive dipeptides in rats

2011 ◽  
Vol 414 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Eduardo M.N. Nakashima ◽  
Akihiro Kudo ◽  
Yuri Iwaihara ◽  
Mitsuru Tanaka ◽  
Kiyoshi Matsumoto ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Stable Isotope ◽  
Tandem Mass Spectrometry ◽  
Isotope Labeling ◽  
Multiple Reaction Monitoring ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Spectrometry Method ◽  
Mass Spectrometry Method

Liquid chromatography–tandem mass spectrometry method for the quantification of a potent H3 receptor antagonist conessine in serum and its application to pharmacokinetic studies

2018 ◽  
Vol 24 (3) ◽  
pp. 289-298
Author(s):  
Mahendra Shukla ◽  
Femi M Francis ◽  
Jawahar Lal
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Multiple Reaction Monitoring ◽  
Pharmacokinetic Studies ◽  
Tandem Mass ◽  
Reaction Monitoring ◽  
Spectrometry Method ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Conessine, a steroidal alkaloid obtained from the bark and seeds of the plant species of Apocynaceae family, elicits a histamine antagonistic action, selectively for the H3 histaminergic receptors. This alkaloid is used mainly for the treatment of dysentery and helminthic disorders. For the quantification of conessine in serum, a liquid chromatography–tandem mass spectrometry method was developed. Chromatographic separation was achieved on a Zorbax SB-CN column (100 × 4.6 mm, 3.5 µm), and a mobile phase consisting of 90% methanol in aqueous ammonium acetate buffer (pH 3.5) with 0.1% (v/v) formic acid at an isocratic flow rate of 0.6 ml/min at 40℃ provides efficiency in separation. A volume of 40 µl was injected each time and the run time for each sample was 5 min. Phenacetin (internal standard) was added to 50 µl of serum sample prior to liquid–liquid extraction using 3% isopropanol in n-hexane. The detection was performed on a 5500 QTRAP mass spectrometer by multiple reaction monitoring mode via electrospray ionization source. The multiple reaction monitoring of conessine and IS was m/ z 357.4 to m/ z 312.1 and m/ z 180.1 to m/ z 138.1, respectively. The method that showed selectivity and linearity in the range of 1–200 ng/ml was validated in terms of sensitivity, accuracy, precision and stability. The detection and quantitation limits were recognized at 0.1 and 1 ng/ml, respectively. The intra- and inter-day precision and accuracy fulfils the acceptance criteria. Applying the method to the pharmacokinetic studies in rats, conessine showed a peak serum concentration at 2 h post oral dose with a good bioavailability of 71.28 ± 4.65%.

Potential enantioselectivity of the hydrolysation and photolysation of the chiral agrochemical penthiopyrad in aquatic environments

2021 ◽  
Author(s):  
Guangqian Yang ◽  
Zhengyi Liu ◽  
Tingting Lan ◽  
Li Dou ◽  
Kankan Zhang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Aqueous Solutions ◽  
Aquatic Environments ◽  
Tandem Mass ◽  
Spectrometry Method ◽  
Mass Spectrometry Method

A liquid chromatography with tandem mass spectrometry method was developed and applied for the investigation of penthiopyrad photolysation and hydrolysation in different aqueous solutions, and the potential enantioselectivity of this chiral fungicide was further assessed.

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