A three-factor Doehlert matrix design in optimising the determination of octadecyltrimethylammonium bromide by cation-exchange chromatography with suppressed conductivity detection

2007 ◽  
Vol 597 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Tommaso R.I. Cataldi ◽  
Donatella Orlando ◽  
Donatella Nardiello ◽  
Alessandra Rubino ◽  
Giuliana Bianco ◽  
...  
Keyword(s):  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Conductivity Detection ◽  
Suppressed Conductivity Detection ◽  
Doehlert Matrix ◽  
Octadecyltrimethylammonium Bromide ◽  
Matrix Design ◽  
Three Factor

Inductively coupled plasma mass spectrometry coupled to cation exchange chromatography for the determination of trace nickel in alkaline electrolyte

2020 ◽  
Vol 35 (7) ◽  
pp. 1295-1299
Author(s):  
Lukas Miner ◽  
Diane Beauchemin
Keyword(s):  
Mass Spectrometry ◽  
Cation Exchange ◽  
Inductively Coupled Plasma ◽  
Exchange Chromatography ◽  
Alkaline Solutions ◽  
Alkaline Electrolyte ◽  
Cation Exchange Chromatography ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry

Cone corrosion by alkaline solutions with flow injection is prevented using cation-exchange chromatography coupled to inductively coupled plasma mass spectrometry.

A method for determination of macropeptide by cation-exchange fast protein liquid chromatography and its use for following the action of chymosin in milk

1991 ◽  
Vol 58 (3) ◽  
pp. 321-328 ◽  
Author(s):  
Joëlle Léonil ◽  
Daniel Mollé
Keyword(s):  
Quantitative Determination ◽  
Cation Exchange ◽  
Soluble Fraction ◽  
Whey Proteins ◽  
Skim Milk ◽  
Exchange Chromatography ◽  
Chromatographic Technique ◽  
Cation Exchange Chromatography ◽  
Preparative Scale

SummaryCation-exchange chromatography on a Mono S column (Pharmacia) was used to separate macropeptide from whey proteins. Macropeptide was eluted by 0·1 M-NaCl in a 20 mM-KCl–HCl buffer, pH 2. This technique was suitable for quantitative determination of macropeptide in rennet whey and also for following the action of chymosin on κ-casein in skim milk. Precipitation at pH 4·6 was used to remove residual caseins and to keep macropeptide in solution. In comparison with other methods for determining macropeptide, the present one eliminates the need for pretreatment of samples with trichloroacetic acid (TCA) and allows the recovery of all the macropeptide. Quantitative determination of macropeptide in the 8% TCA-soluble fraction by cation-exchange chromatography showed that only 50–75% of the macropeptide was recovered. This chromatographic technique could also be applied for isolating and producing whole macropeptide on a preparative scale.

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