Molecularly imprinted polymer microspheres prepared by Pickering emulsion polymerization for selective solid-phase extraction of eight bisphenols from human urine samples

A rapid, selective and reliable sample preparation technique employing solid-phase extraction (SPE) based on molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) in human urine was described. After sample adjustment to pH 2.5 with 0.1 M HCl, the urine sample was loaded onto the MIP-SPE column, and after a wash step, OTA was eluted for measurement by ultra-high performance liquid chromatography coupled with fluorescence detection. Key parameters which affected the MIP-SPE extraction efficiency were optimized as was the detection method. Under the optimised conditions, the limits of detection and quantification for OTA in urine were 0.2 ng/ml and 0.6 ng/ml, respectively. The recoveries for OTA in urine, spiked at the 0.6, 6.0 and 60 ng/ml levels, ranged from 92.0 to 98.9%. Sixty urine samples were analysed, of which four were found to contain OTA at concentrations ranging from 0.022 to 0.083 ng/ml; the positive results were confirmed by liquid chromatography coupled with tandem mass spectrometry. OTA determination in urine is a good indicator for human exposure to the mycotoxin, and this is the first report on OTA contamination in Chinese people.

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Computational design of molecularly imprinted polymer for solid phase extraction of moxifloxacin hydrochloride from Avalox® tablets and spiked human urine samples

Microchemical Journal ◽

10.1016/j.microc.2019.04.063 ◽

2019 ◽

Vol 148 ◽

pp. 51-56 ◽

Cited By ~ 3

Author(s):

Mennatallah A. Hammam ◽

Mohammad Abdel-Halim ◽

Adel Madbouly ◽

Hebatallah A. Wagdy ◽

Rasha M. El Nashar

Keyword(s):

Solid Phase Extraction ◽

Molecularly Imprinted Polymer ◽

Human Urine ◽

Solid Phase ◽

Computational Design ◽

Urine Samples ◽

Phase Extraction ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Spiked Human Urine

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Synthesis and application of molecularly imprinted polymer for highly selective solid phase extraction trace amount of sotalol from human urine samples: Optimization by central composite design (CCD)

Medicinal Chemistry Research ◽

10.1007/s00044-017-1947-1 ◽

2017 ◽

Vol 26 (10) ◽

pp. 2477-2490 ◽

Cited By ~ 14

Author(s):

Saeedeh Ansari ◽

Majid Karimi

Keyword(s):

Solid Phase Extraction ◽

Central Composite Design ◽

Molecularly Imprinted Polymer ◽

Human Urine ◽

Trace Amount ◽

Solid Phase ◽

Phase Extraction ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Central Composite

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Assessment of the Performance of Solid Phase Extraction Based on Pipette Tip Employing a Hybrid Molecularly Imprinted Polymer as an Adsorbent for Enantioselective Determination of Albendazole Sulfoxide

Journal of Chromatographic Science ◽

10.1093/chromsci/bmz036 ◽

2019 ◽

Vol 57 (7) ◽

pp. 671-678 ◽

Cited By ~ 1

Author(s):

Sara da Silva Anacleto ◽

Hanna Leijoto de Oliveira ◽

Anny Talita Maria da Silva ◽

Tienne Aparecida do Nascimento ◽

Marcella Matos Cordeiro Borges ◽

...

Keyword(s):

Solid Phase Extraction ◽

Molecularly Imprinted Polymer ◽

Human Urine ◽

Solid Phase ◽

Phase Extraction ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Albendazole Sulfoxide ◽

Pipette Tip

Abstract Herein, an organic–inorganic hybrid molecularly imprinted polymer (MIP) was successfully synthesized with albendazole sulfoxide (ABZSO) as a template and 3-(trimethoxysilyl)propyl methacrylate, a bifunctional group compound, as a single cross-linking agent. In this study, a simple method using HPLC–DAD was developed for the determination of ABZSO enantiomers in human urine using pipette tip-based molecularly imprinted polymer solid phase extraction (PT–MIP–SPE). Enantioseparation with satisfactory retention times (5.17 and 7.09 min), acceptable theoretical plates (N = 4,535 and 5,091) and strong resolution (Rs = 5.45) was performed with an Agilent® Eclipse Plus C18 (100 mm × 4.6 mm, 3.5 μm) coupled with a Chiralpak® IA column (100 mm × 4.6 mm, 3 μm), a mixture with ethanol:water (50:50, v/v) as the mobile phase, temperature at 40°C, flow rate at 0.9 mL min−1 and λ = 230 nm. Thereafter, certain parameters affecting the PT–MIP–SPE were investigated in detail and the better conditions were: 300 μL of water as washing solvent, 500 μL of ethanol:acetic acid (9:1, v/v) as eluting solvent, 20 mg of MIP, 500 μL of human urine at pH 9 and no addition of NaCl. Recoveries/relative standard deviation (RSD%) for (R)-(+)-ABZSO and (S)-(−)-ABZSO were 78.2 ± 0.2% and 69.7 ± 1.7%, respectively.

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