Sandwich-type electrochemical aptasensor based on hollow mesoporous carbon spheres loaded with porous dendritic Pd@Pt nanoparticles as signal amplifier for ultrasensitive detection of cardiac troponin I

2021 ◽  
pp. 339202
Author(s):  
Zhenxing Wang ◽  
Hongli Zhao ◽  
Kaicha Chen ◽  
Hongyuan Li ◽  
Minbo Lan
Keyword(s):  
Cardiac Troponin ◽  
Mesoporous Carbon ◽  
Troponin I ◽  
Pt Nanoparticles ◽  
Cardiac Troponin I ◽  
Ultrasensitive Detection ◽  
Electrochemical Aptasensor ◽  
Carbon Spheres ◽  
Signal Amplifier ◽  
Sandwich Type

Autoantibody prevalence with an improved immunoassay for detecting cardiac troponin-specific autoantibodies

2014 ◽  
Vol 52 (2) ◽  
Author(s):  
Tanja Savukoski ◽  
Tuomo Ilva ◽  
Juha Lund ◽  
Pekka Porela ◽  
Noora Ristiniemi ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Emergency Department ◽  
Chest Pain ◽  
Cardiac Troponin ◽  
Troponin I ◽  
State Of The Art ◽  
Cardiac Troponin I ◽  
High Prevalence ◽  
Sandwich Type ◽  
Pain Patients

AbstractCardiac troponin-specific autoantibodies (cTnAAb) can interfere with the measurement of cardiac troponin I (cTnI) by immunoassays used for the diagnosis of myocardial infarction (MI). Here, an improved version of a previous autoantibody assay was validated and used to evaluate the cTnAAb prevalence in a cohort of consecutive chest pain patients presenting to an emergency department.Admission samples from 510 patients with suspected MI were analyzed in parallel with two sandwich-type cTnAAb assays based on different cTnI epitopes used to capture cardiac troponin-bound cTnAAbs.Sample-specific backgrounds were lower for the new assay than for the old assay (median 1225 vs. 2693 counts, p<0.001). Net signals of cTnAAb-positive samples were higher for the new assay than for the old assay (median 5076 vs. 3921 counts, p<0.001). Of all patients, 9.2% were cTnAAb-positive for the new assay and 7.3% for the old assay (p=0.013). Previous cardiac problems were not associated with cTnAAb status and cTnAAb status did not correlate with the 12-month outcome.With our new and more sensitive autoantibody assay, approximately one out of ten patients who presented to the initial cardiac triage had detectable amounts of cTnAAbs in the circulation. Because these cTnAAbs can interfere with state-of-the-art cTnI assays, their high prevalence should be acknowledged by clinical chemists, physicians, and kit manufacturers.

Electrochemical Aptasensor of Cardiac Troponin I for the Early Diagnosis of Acute Myocardial Infarction

2015 ◽  
Vol 87 (19) ◽  
pp. 9869-9875 ◽  
Author(s):  
Hunho Jo ◽  
Hyunwoo Gu ◽  
Weejeong Jeon ◽  
Hyungjun Youn ◽  
Jin Her ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Early Diagnosis ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Cardiac Troponin I ◽  
Electrochemical Aptasensor

scholarly journals Spiegelmers as potential receptors for cTnI diagnostics

2017 ◽  
Vol 9 (35) ◽  
pp. 5091-5093 ◽  
Author(s):  
Zsuzsanna Szeitner ◽  
Anna Doleschall ◽  
Marina Varga ◽  
Katalin Keltai ◽  
Katalin Révész ◽  
...  
Keyword(s):  
Blood Serum ◽  
Cardiac Troponin ◽  
Quantitative Assessment ◽  
Troponin I ◽  
Cardiac Troponin I ◽  
Serum Samples ◽  
Affinity Assay ◽  
Sandwich Type

We demonstrate the first application of a nuclease resistant aptamer, the so-called Spiegelmer, in a sandwich-type affinity assay by quantitative assessment of cardiac Troponin I (cTnI) in blood serum samples.

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