Easy flat embedding of oriented samples in hydrophilic resin (LR White) under controlled atmosphere: Application allowing both nucleic acid hybridizations (CARD-FISH) and ultrastructural observations

2008 ◽  
Vol 110 (5) ◽  
pp. 427-431 ◽  
Author(s):  
Olivier Gros ◽  
Leslie C. Maurin
Keyword(s):  
Nucleic Acid ◽  
Controlled Atmosphere ◽  
Lr White ◽  
Oriented Samples ◽  
Flat Embedding ◽  
Card Fish

Flat embedding of microorganisms on slides with LR white for immunocytochemistry

1993 ◽  
Vol 26 (4) ◽  
pp. 352-353 ◽  
Author(s):  
Peter A. Vesk ◽  
Teresa P. Dibbayawan ◽  
Maret Vesk
Keyword(s):  
Lr White ◽  
Flat Embedding

Two Methods for Flat Embedding Sections in LR White Cut from Paraffin Blocks

1991 ◽  
Vol 66 (2) ◽  
pp. 89-92 ◽  
Author(s):  
Antonio Migheli ◽  
Angelo Attanasio
Keyword(s):  
Lr White ◽  
Flat Embedding

scholarly journals LR White Flat Embedding of Cells Grown on Coverslips

2000 ◽  
Vol 8 (9) ◽  
pp. 34-35
Author(s):  
Tamara Howard
Keyword(s):  
Lr White ◽  
To Come ◽  
Flat Embedding

We do this two different ways, using polyethylene (PE) molds or aluminum weigh boats, depending on the sample. You can either heat- or UV-cure these molds – just change the type of “lid” on the embedding mold.The PE molds are used when the coverslip can be made to fit in the mold. We use JB-4-type molds, available from your favorite microscopy supplier. The key to using these molds is that they have to be pre-treated…fill the inner cup of the mold with LR White, cover with an aluminum JB-4 chuck (try to have enough resin in the mold to come up around the base of the chuck), and polymerize in a 60° C oven for a day or two, until the resin is hard. The Al chuck can usually be removed by hand, but a flathead screwdriver used as a pry will help pop it off if you have trouble.

scholarly journals LR White Flat Embedding Of Cells Grown On Coverslips

2000 ◽  
Vol 8 (10) ◽  
pp. 26-26
Author(s):  
Tamara Howard
Keyword(s):  
Lr White ◽  
To Come ◽  
Flat Embedding

We do this two different ways, using polyethylene (PE) molds or aluminum weigh boats, depending on the sample, You can either heat- or UV-cure these molds-just change the type of “lid” on the embedding mold.The PE molds are used when the coverslip can be made to fit in the mold. We use JB-4-type molds, available from your favorite microscopy supplier. The key to using these molds is that they have to be pre-treated...fill the inner cup of the mold with LR White, cover with an aluminum JB-4 chuck (try to have enough resin in the mold to come up around the base of the chuck), and polymerize in a 60° C oven for a day or two, until the resin is hard.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

Infection of Escherichia coli with bacteriophages

1969 ◽  
Vol 27 ◽  
pp. 370-371
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Cell Surface ◽  
Virus Type ◽  
Infection Process ◽  
Adsorption Site ◽  
The Other ◽  
Specific Receptors ◽  
Bacterial Receptors

The first step in the infection of a bacterium by a virus consists of a collision between cell and bacteriophage. The presence of virus-specific receptors on the cell surface will trigger a number of events leading eventually to release of the phage nucleic acid. The execution of the various "steps" in the infection process varies from one virus-type to the other, depending on the anatomy of the virus. Small viruses like ØX 174 and MS2 adsorb directly with their capsid to the bacterial receptors, while other phages possess attachment organelles of varying complexity. In bacteriophages T3 (Fig. 1) and T7 the small conical processes of their heads point toward the adsorption site; a welldefined baseplate is attached to the head of P22; heads without baseplates are not infective.

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