In situ flat embedding of monolayers and cell relocation in the acrylic resin LR White for comparative light and electron microscopy studies

1994 ◽  
Vol 26 (12) ◽  
pp. 934-938 ◽  
Author(s):  
Marianne Steiner ◽  
Christian Schöfer ◽  
Wilhelm Mosgoeller
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Acrylic Resin ◽  
Lr White ◽  
Flat Embedding

Flat embedding and immunolabelling of SW 1116 colon carcinoma cells in LR white: An improved technique in light and electron microscopy

1992 ◽  
Vol 21 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Gertrud Goping ◽  
Saul Yedgar ◽  
Harvey B. Pollard ◽  
Gemma A. J. Kuijpers
Keyword(s):  
Electron Microscopy ◽  
Colon Carcinoma ◽  
Light And Electron Microscopy ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Lr White ◽  
Flat Embedding ◽  
Improved Technique

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

scholarly journals Localization of ‘Candidatus Liberibacter solanacearum’ and Evidence for Surface Appendages in the Potato Psyllid Vector

2016 ◽  
Vol 106 (2) ◽  
pp. 142-154 ◽  
Author(s):  
J. M. Cicero ◽  
T. W. Fisher ◽  
J. K. Brown
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Fluorescence Labeling ◽  
Potato Psyllid ◽  
Bactericera Cockerelli ◽  
Visual Identification ◽  
Transmission Electron ◽  
Candidatus Liberibacter ◽  
Identification Of Bacteria

The potato psyllid Bactericera cockerelli is implicated as the vector of the causal agent of zebra chip of potato and vein-greening of tomato diseases. Until now, visual identification of bacteria in the genus ‘Candidatus Liberibacter’ has relied on direct imaging by light and electron microscopy without labeling, or with whole-organ fluorescence labeling only. In this study, aldehyde fixative followed by a coagulant fixative, was used to process adult psyllids for transmission electron microscopy (TEM) colloidal gold in situ hybridization experiments. Results indicated that ‘Ca. Liberibacter solanacearum’ (CLso)-specific DNA probes annealed to a bacterium that formed extensive, monocultural biofilms on gut, salivary gland, and oral region tissues, confirming that it is one morphotype of potentially others, that is rod-shaped, approximately 2.5 µm in diameter and of variable length, and has a rough, granular cytosol. In addition, CLso, prepared from shredded midguts, and negatively stained for TEM, possessed pili- and flagella-like surface appendages. Genes implicating coding capacity for both types of surface structures are encoded in the CLso genome sequence. Neither type was seen for CLso associated with biofilms within or on digestive organs, suggesting that their production is stimulated only in certain environments, putatively, in the gut during adhesion leading to multiplication, and in hemolymph to afford systemic invasion.

scholarly journals A Novel Flat-embedding Method to Prepare Ultrathin Cryosections from Cultured Cells in Their In Situ Orientation

2002 ◽  
Vol 50 (8) ◽  
pp. 1067-1080 ◽  
Author(s):  
Viola Oorschot ◽  
Heidi de Wit ◽  
Wim G. Annaert ◽  
Judith Klumperman
Keyword(s):  
Electron Microscopy ◽  
Quantitative Method ◽  
Cultured Cells ◽  
Petri Dish ◽  
Ultrastructural Level ◽  
Immunogold Labeling ◽  
Polarized Cells ◽  
Ultrathin Cryosections ◽  
Flat Embedding

Immunogold labeling of ultrathin cryosections provides a sensitive and quantitative method to localize proteins at the ultrastructural level. An obligatory step in the routine preparation of cryosections from cultured cells is the detachment of cells from their substrate and subsequent pelleting. This procedure precludes visualization of cells in their in situ orientation and hampers the study of polarized cells. Here we describe a method to sample cultured cells from a petri dish or coverslip by embedding them in a 12% gelatin slab. Subsequently, sections can be prepared in parallel or perpendicular to the plane of growth. Our method extends the cryosectioning technique to applications in studying polarized cells and correlative light–electron microscopy.

A Simple Procedure for in Situ Immunolabeling, Embedding and Sectioning of Layers of Cultured Endothelial and Smooth Muscle Cells for both Light and Electron Microscopy

1997 ◽  
Vol 72 (1) ◽  
pp. 45-48
Author(s):  
Andre B. Mulder ◽  
Nel R. Blom ◽  
Jan van der Meer ◽  
M. Ruud Halie ◽  
Jan W. Smit
Keyword(s):  
Electron Microscopy ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Simple Procedure ◽  
Light And Electron Microscopy ◽  
Muscle Cells

scholarly journals Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ

2017 ◽  
Vol 3 (1-3) ◽  
pp. 8-16 ◽  
Author(s):  
Shengliu Wang ◽  
Shuoguo Li ◽  
Gang Ji ◽  
Xiaojun Huang ◽  
Fei Sun
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy

COMPARATIVE EFFECT OF INSULIN ON EXPLANTED ADRENAL MEDULLARY TISSUE AND RAT ADRENAL MEDULLA IN SITU

1975 ◽  
Vol 64 (2) ◽  
pp. 323-NP ◽  
Author(s):  
R. S. PIEZZI ◽  
L. A. POHORECKY ◽  
J. C. CAVICCHIA ◽  
J. P. GALLEANO
Keyword(s):  
Electron Microscopy ◽  
Anterior Chamber ◽  
Adrenal Medulla ◽  
Chromaffin Cells ◽  
Insulin Treatment ◽  
Light And Electron Microscopy ◽  
Comparative Effect ◽  
Medullary Tissue ◽  
Releasing Effect

SUMMARY The adrenal medulla and explanted medullary cells in the anterior chamber of the eye were examined 3 h after the administration of insulin to rats. No alterations were seen in the explanted cells. In the adrenal medulla, light and electron microscopy indicated depletion of adrenaline cells; noradrenaline cells were not affected. Levels of catecholamines were three times higher in the explanted tissue after insulin treatment; in the medulla in situ they declined by 70%. These results confirm that insulin has no direct releasing effect on chromaffin cells.

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