scholarly journals Demonstration of Tissue Resident Memory CD8 T Cells in Insulitic Lesions in Adult Patients with Recent-Onset Type 1 Diabetes

2017 ◽  
Vol 187 (3) ◽  
pp. 581-588 ◽  
Author(s):  
Enida Kuric ◽  
Peter Seiron ◽  
Lars Krogvold ◽  
Bjørn Edwin ◽  
Trond Buanes ◽  
...  
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
Cd8 T Cells ◽  
Adult Patients ◽  
Memory Cd8 T Cells ◽  
Recent Onset ◽  
Onset Type

scholarly journals One in Ten CD8+ Cells in the Pancreas of Living Individuals With Recent Onset Type 1 Diabetes Recognizes the Preproinsulin Epitope PPI15-24

2021 ◽  
Author(s):  
Ada Admin ◽  
Teresa Rodriguez-Calvo ◽  
Lars Krogvold ◽  
Natalie Amirian ◽  
Knut Dahl-Jørgensen ◽  
...  
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
T Cell ◽  
Cd8 T Cells ◽  
Pancreatic Tissue ◽  
Cell Reactivity ◽  
Tissue Samples ◽  
Recent Onset ◽  
Onset Type

In type 1 diabetes, a lifelong autoimmune disease, T cells infiltrate the islets and the exocrine pancreas in high numbers. CD8+ T cells are the main cell type found in the insulitic lesion, and CD8+ T cells reactive against beta cell antigens have been detected in the periphery and in the pancreas of subjects with short and long disease duration. The Diabetes Virus Detection (DiViD) study collected pancreatic tissue, by pancreatic tail resection, from living patients with recent-onset type 1 diabetes. These tissues have been extensively studied by the scientific community, but the autoreactive nature of the T cell infiltrate has remained unexplored. Our objective was to determine the number and localization of these cells in pancreas samples obtained through the DiViD study. Here, we demonstrate the presence of high frequencies of CD8+ T cells reactive against a highly relevant epitope derived from the preproinsulin signal peptide in pancreatic tissue samples from these donors. We additionally show the heterogeneity of islet distribution and CD8+ T cell infiltration. Our findings contribute to the current limited existing knowledge on T cell reactivity in the pancreas of recent onset type 1 diabetic donors, and indicate that antigen-specific therapies directed towards preproinsulin could have high clinical impact.

scholarly journals One in Ten CD8+ Cells in the Pancreas of Living Individuals With Recent Onset Type 1 Diabetes Recognizes the Preproinsulin Epitope PPI15-24

2021 ◽  
Author(s):  
Ada Admin ◽  
Teresa Rodriguez-Calvo ◽  
Lars Krogvold ◽  
Natalie Amirian ◽  
Knut Dahl-Jørgensen ◽  
...  
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
T Cell ◽  
Cd8 T Cells ◽  
Pancreatic Tissue ◽  
Cell Reactivity ◽  
Tissue Samples ◽  
Recent Onset ◽  
Onset Type

In type 1 diabetes, a lifelong autoimmune disease, T cells infiltrate the islets and the exocrine pancreas in high numbers. CD8+ T cells are the main cell type found in the insulitic lesion, and CD8+ T cells reactive against beta cell antigens have been detected in the periphery and in the pancreas of subjects with short and long disease duration. The Diabetes Virus Detection (DiViD) study collected pancreatic tissue, by pancreatic tail resection, from living patients with recent-onset type 1 diabetes. These tissues have been extensively studied by the scientific community, but the autoreactive nature of the T cell infiltrate has remained unexplored. Our objective was to determine the number and localization of these cells in pancreas samples obtained through the DiViD study. Here, we demonstrate the presence of high frequencies of CD8+ T cells reactive against a highly relevant epitope derived from the preproinsulin signal peptide in pancreatic tissue samples from these donors. We additionally show the heterogeneity of islet distribution and CD8+ T cell infiltration. Our findings contribute to the current limited existing knowledge on T cell reactivity in the pancreas of recent onset type 1 diabetic donors, and indicate that antigen-specific therapies directed towards preproinsulin could have high clinical impact.

scholarly journals CD4 CD25 T Cells in Children with Recent Onset Type 1 Diabetes

2021 ◽  
Vol 89 (6) ◽  
pp. 1079-1087
Author(s):  
MARWA FATHY, M.Sc.; IBRAHIM IBRAHIM M. EL ARABY, M.D. ◽  
NANCY M. EL GUINDY, M.D.; GHADA M. ANWAR, M.D.
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
Recent Onset ◽  
Onset Type

scholarly journals Intralymphatic GAD-alum Injection Modulates B Cell Response and Induces Follicular Helper T Cells and PD-1+ CD8+ T Cells in Patients With Recent-Onset Type 1 Diabetes

2022 ◽  
Vol 12 ◽  
Author(s):  
Hugo Barcenilla ◽  
Mikael Pihl ◽  
Jeanette Wahlberg ◽  
Johnny Ludvigsson ◽  
Rosaura Casas
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
B Cells ◽  
Lymph Nodes ◽  
Cd8 T Cells ◽  
Helper T Cells ◽  
Follicular Helper T Cells ◽  
Recent Onset ◽  
Follicular Helper

Antigen-specific immunotherapy is an appealing strategy to preserve beta-cell function in type 1 diabetes, although the approach has yet to meet its therapeutic endpoint. Direct administration of autoantigen into lymph nodes has emerged as an alternative administration route that can improve the efficacy of the treatment. In the first open-label clinical trial in humans, injection of aluminum-formulated glutamic acid decarboxylase (GAD-alum) into an inguinal lymph node led to the promising preservation of C-peptide in patients with recent-onset type 1 diabetes. The treatment induced a distinct immunomodulatory effect, but the response at the cell level has not been fully characterized. Here we used mass cytometry to profile the immune landscape in peripheral blood mononuclear cells from 12 participants of the study before and after 15 months of treatment. The immunomodulatory effect of the therapy included reduction of naïve and unswitched memory B cells, increase in follicular helper T cells and expansion of PD-1+ CD69+ cells in both CD8+ and double negative T cells. In vitro stimulation with GAD65 only affected effector CD8+ T cells in samples collected before the treatment. However, the recall response to antigen after 15 months included induction of CXCR3+ and CD11c+Tbet+ B cells, PD-1+ follicular helper T cells and exhausted-like CD8+ T cells. This study provides a deeper insight into the immunological changes associated with GAD-alum administration directly into the lymph nodes.

Pathophysiological characteristics of preproinsulin-specific CD8+ T cells in subjects with juvenile-onset and adult-onset type 1 diabetes: A 1-year follow-up study

2017 ◽  
Vol 19 (1) ◽  
pp. 68-79 ◽  
Author(s):  
Mahinder Paul ◽  
Darshan Badal ◽  
Neenu Jacob ◽  
Devi Dayal ◽  
Rakesh Kumar ◽  
...  
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
Cd8 T Cells ◽  
Adult Onset ◽  
Juvenile Onset ◽  
Onset Type ◽  
Follow Up Study

Inducible regulatory T cells (iTregs) from recent-onset type 1 diabetes subjects show increased in vitro suppression and higher ITCH levels compared with controls

2010 ◽  
Vol 339 (3) ◽  
pp. 585-595 ◽  
Author(s):  
Sanja Glisic ◽  
Sarah Ehlenbach ◽  
Parthav Jailwala ◽  
Jill Waukau ◽  
Srikanta Jana ◽  
...  
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
Regulatory T Cells ◽  
Recent Onset ◽  
Onset Type

scholarly journals Increased Effector Memory Insulin-Specific CD4 + T Cells Correlate With Insulin Autoantibodies in Patients With Recent-Onset Type 1 Diabetes

Diabetes ◽  
2017 ◽  
Vol 66 (12) ◽  
pp. 3051-3060 ◽  
Author(s):  
Justin A. Spanier ◽  
Nathanael L. Sahli ◽  
Joseph C. Wilson ◽  
Tijana Martinov ◽  
Thamotharampillai Dileepan ◽  
...  
Keyword(s):  
T Cells ◽  
Type 1 Diabetes ◽  
Cd4 T Cells ◽  
Insulin Autoantibodies ◽  
Effector Memory ◽  
Recent Onset ◽  
Onset Type

Precursor frequencies of T-cells reactive to insulin in recent onset type 1 diabetes mellitus

2004 ◽  
Vol 23 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Ramachandra G Naik ◽  
Cora Beckers ◽  
Rino Wentwoord ◽  
Arlette Frenken ◽  
Gaby Duinkerken ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
T Cells ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Recent Onset ◽  
Onset Type
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