Enteric methane production, digestibility and rumen fermentation in dairy cows fed different forages with and without rapeseed fat supplementation

2013 ◽  
Vol 184 (1-4) ◽  
pp. 67-79 ◽  
Author(s):  
M. Brask ◽  
P. Lund ◽  
A.L.F. Hellwing ◽  
M. Poulsen ◽  
M.R. Weisbjerg
2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.


2009 ◽  
Vol 92 (6) ◽  
pp. 2809-2821 ◽  
Author(s):  
L. Holtshausen ◽  
A.V. Chaves ◽  
K.A. Beauchemin ◽  
S.M. McGinn ◽  
T.A. McAllister ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0140282 ◽  
Author(s):  
Jolien B. Veneman ◽  
Stefan Muetzel ◽  
Kenton J. Hart ◽  
Catherine L. Faulkner ◽  
Jon M. Moorby ◽  
...  

2015 ◽  
Vol 207 ◽  
pp. 10-19 ◽  
Author(s):  
Lene Alstrup ◽  
Anne Louise Frydendahl Hellwing ◽  
Peter Lund ◽  
Martin Riis Weisbjerg

2012 ◽  
Vol 52 (7) ◽  
pp. 653 ◽  
Author(s):  
L. Li ◽  
J. Davis ◽  
J. Nolan ◽  
R. Hegarty

The effects of dietary nitrate and of urea on rumen fermentation pattern and enteric methane production were investigated using 4-month-old ewe lambs. Ten lambs were allocated into two groups (n = 5) and each group was offered one of two isonitrogenous and isoenergetic diets containing either 1.5% urea (T1) or 3% calcium nitrate (T2). Methane production was estimated using open-circuit respiration chambers after 6 weeks of feeding. No difference in nitrogen (N) balance, apparent digestibility of N or microbial N outflow existed between treatments (P > 0.05). Animals offered the T2 diet lost less energy through methane than did those fed the T1 diet (P < 0.05). Total volatile fatty acid concentration, molar proportion of propionate, and the molar ratio of acetate to propionate in rumen fluid were not affected by dietary N source. Compared with urea inclusion, nitrate inclusion caused a significantly higher acetate and lower butyrate percentage in rumen volatile fatty acid. Nitrate supplementation tended to lower methane production by ~7.7 L/day relative to urea supplementation (P = 0.06). Methane yield (L/kg DM intake) was reduced (P < 0.05) by 35.4% when 1.5% urea was replaced by 3% calcium nitrate in the diet. Emission intensity (L methane/kg liveweight gain) was ~17.3% lower in the nitrate-supplemented sheep when compared with urea-fed sheep; however, the reduction was not statistically significant (P > 0.05). This study confirms that the presence of nitrate in the diet inhibits enteric methane production. As no clinical symptoms of nitrite toxicity were observed and sheep receiving nitrate-supplemented diet had similar growth to those consuming urea-supplemented diet, it is concluded that 3% calcium nitrate can replace 1.5% urea as a means of meeting ruminal N requirements and of reducing enteric methane emissions from sheep, provided animals are acclimated to nitrate gradually.


2015 ◽  
Vol 98 (10) ◽  
pp. 7248-7263 ◽  
Author(s):  
L.J. Watt ◽  
C.E.F. Clark ◽  
G.L. Krebs ◽  
C.E. Petzel ◽  
S. Nielsen ◽  
...  

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