334 Effect of Dietary Supplementation of Peanut Skins with and Without Polyphenols on the Performance, Rumen Fermentation and Carcass Characteristics of Florida-native Sheep

The objective of this study was to evaluate the effects of dietary supplementation of peanut skins (PS) with and without polyphenols on the performance, rumen fermentation, and carcass characteristics of Florida-Native Sheep. Thirty-three intact lambs were randomly blocked by body weight (BW) (33.5 ± 0.1 kg; 11 lambs per treatment) and distributed in three isonitrogenous (17.1%) and isocaloric (2.5 Mcal/kg) diets: 1) Control diet, 2) Diet + 20% of intact peanut skins (IPS) and, 3) Diet + 20% of treated PS without polyphenols (TPS) for 42 d + 7 days of adaptation. Then, lambs were slaughtered to evaluate carcass characteristics and rumen fermentation. Dry matter intake (DMI), BW, and blood samples were collected and analyzed using the effects of treatment, week, interactions, and the random effect of animals. Dietary IPS and TPS did not (P > 0.05) affect DMI (1.14, 1.15 vs 1.04 kg/d) and final BW (35.9, 35.8 vs 36.8 kg) compared to the control. Dietary TPS reduced (P < 0.01) blood glucose levels compared to IPS and control (67.9 vs 74.6, 75.7 mg/dL) but IPS had lower (P = 0.03) blood urea nitrogen than TPS and control (5.79 vs 6.0, 6.32 mmol/L). Both IPS and TPS decreased (P < 0.01) total volatile fatty acid concentration (37.9, 14.2 vs 45.8 mmol/L) and increased rumen pH (7.1, 6.9 vs 6.7) compared to the control. Carcass weight was similar between treatments, TPS reduced (P = 0.01) dressing percentage compared to IPS and control (42.6 vs 45.2, 46.6%), both IPS and TPS reduced liver weight compared to control (230, 223 vs 302 g). Conversely, IPS and TPS increased (P < 0.01) alpha-tocopherol concentration (3.73, 4.0 vs 1.24 mg/g) in lean meat compared to control. This study showed that IPS increased the incorporation of antioxidants in meat without affecting animal performance.

Review of Soft Sensors in Anaerobic Digestion Process

Anaerobic digestion is associated with various crucial variables, such as biogas yield, chemical oxygen demand, and volatile fatty acid concentration. Real-time monitoring of these variables can not only reflect the process of anaerobic digestion directly but also accelerate the efficiency of resource conversion and improve the stability of the reaction process. However, the current real-time monitoring equipment on the market cannot be widely used in the industrial production process due to its defects such as expensive equipment, low accuracy, and lagging analysis. Therefore, it is essential to conduct soft sensor modeling for unmeasurable variables and use auxiliary variables to realize real-time monitoring, optimization, and control of the an-aerobic digestion process. In this paper, the basic principle and process flow of anaerobic digestion are first briefly introduced. Subsequently, the development history of the traditional soft sensor is systematically reviewed, the latest development of soft sensors was detailed, and the obstacles of the soft sensor in the industrial production process are discussed. Finally, the future development trend of deep learning in soft sensors is deeply discussed, and future research directions are provided.

Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

Background: The major GHG from ruminants is enteric methane (CH4) which in 2010, was estimated at 2.1 Gt of CO2 equivalent, accounting for 4.3% of global GHG emissions. There re extensive efforts being made around the world to develop methane mitigating inhibitors that specifically target rumen methanogens with the ultimate goal of reducing the environmental footprint of ruminant livestock production. This study examined the individual and combined effects of supplementing a high-forage diet (90% barley silage) fed to beef cattle with the investigational methane (CH4) inhibitor 3-nitrooxypropanol (3-NOP) and canola oil (OIL) on the rumen microbial community in relation to enteric CH4 emissions and ruminal fermentation.Results: 3-NOP and OIL individually reduced enteric CH4 emission (-28.2% and -24.0%, respectively), and the effects were additive when used in combination (-51.3%). 3-NOP increased H2 emissions 37 fold, while co-administering 3-NOP and OIL increased H2 in the rumen 20-fold relative to the control diet. The inclusion of 3-NOP or OIL reduced the diversity of the rumen microbiome. 3-NOP resulted in targeted changes in the microbiome decreasing the relative abundance of Methanobrevibacter and increasing the relative abundance of Bacteroidetes. The inclusion of OIL resulted in large scale changes to the microbial community that were associated with changes in ruminal volatile fatty acid concentration and gas production. OIL significantly reduced the abundance of protozoa and fiber-degrading microbes in the rumen but it did not selectively alter the abundance of rumen methanogens.Conclusions: Our data provide a mechanistic understanding of CH4 inhibition by 3-NOP and OIL when fed alone and in combination to cattle. 3-NOP specifically targeted rumen methanogens inhibiting the hydrogenotrophic methanogenesis pathway and resulting in increased H2 emissions and propionate production. In contrast, OIL caused large scale changes in the rumen microbial community by indiscriminately altering the abundance of a range of rumen microbes, reducing the abundance of fibrolytic bacteria and altering rumen fermentation. Importantly, our data suggests that co-administering CH4 inhibitors with distinct mechanisms of action can both enhance CH4 inhibition and provide alternative sinks to prevent excessive accumulation of ruminal H2.

Garlic skin induced shifts in the rumen microbiome and metabolome of fattening sheep

Background Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This study was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, ruminal microbes and metabolites in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. In addition, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

Garlic Skin Induced Shifts in the Rumen Microbiome and Metabolome of Fattening Sheep

Background: Garlic and its constituents exhibit activities on modifying rumen fermentation and improving growth performance. As a by-product of garlic processing, garlic skin contains similar bioactive components as garlic bulb. However, studies in ruminants using garlic skin are scarce. This experiment was conducted to investigate the effects of garlic skin supplementation on rumen fermentation characterizes, growth performance, and involved mechanism in ruminants. Twelve Hu lambs were randomly assigned into one of two treatments: basal diet (CON) or basal diet supplemented with 80 g/kg DM of gallic skin (GAS). The experiment lasted for10 weeks, with the first 2 weeks for adaptation. Results: The results revealed that the average daily gain and volatile fatty acid concentration were higher (P < 0.05) in lambs fed GAS than that in the control group. Garlic skin supplementation did not significantly (P > 0.10) affect the α-diversity indices. Increased (P < 0.05) abundances of Prevotella, Bulleidia, Howardella, Methanosphaera but a decreased (P < 0.05) abundance of Fretibacterium were observed in GAS-fed lambs. Besides, the garlic skin supplementation favorably regulated (P < 0.05) pyrimidine metabolism, purine metabolism, vitamin B6 and B1 metabolism. Moreover, high correlations were observed between fluctuant rumen microbiota and metabolites. Conclusions: Supplementation of garlic skin improved the growth performance of sheep by modifying rumen fermentation through inducing shifts in the rumen microbiome and metabolome.

Effect of High Sulfur Diet on Rumen Fermentation, Microflora and Epithelial Barrier Function in Steers

Background: Sulfur is essential for maintaining animal health and functional processing, but high sulfur in the diet can be toxic. The underlying mechanism for this toxicity is not clear in ruminants. Thus, we conducted experiments to evaluate the effect of excessive sulfur on rumen fermentation, microflora, and epithelial barrier function in steers through in vitro gas production and animal feeding trials.Results: The results showed that increasing the level of sulfur in substrates, decreased the total gas and methane production linearly while increasing the hydrogen sulfide gas (P<0.01). Though the high sulfur diet did not affect the rumen fermentation parameters (P>0.05), the volatile fatty acid concentration, especially the butyric acid, was increased by extra sulfur (P<0.01). Sulfur content in the diet had no significant effect (P>0.05) on most of the rumen microbes, expect Desulfovibrio, one of the major sulfate-reducing bacteria (SRB) in the rumen, whose population increased by adding extra sulfur (P<0.001). Changes in the morphology of rumen epithelium and thickening of the total epithelial layer were mainly attributed to the increase in the acanthosis cell layer and stratum basale (P<0.05). Also, the relative expression of two tight junction protein regulating genes, CLDN-1 and TJP1, were reduced with an increase in sulfur level in the diet (P<0.05).Conclusion: These results indicate that excessive sulfur in the diet can change the type of rumen fermentation, sulfate metabolism and SRB population, and the rumen epithelial barrier function.

Pengaruh substitusi fodder jagung pada silase rumput alam terhadap konsumsi dan kecernaan serat, konsentrasi VFA dan kadar glukosa darah pada pedet jantan sapi persilangan ongole x brahman lepas sapih

The objective of this experiment was to evaluate the effect of corn fodder on intake and fiber digestibility, volatile fatty acid and blood glucose concentration of weaned Ongole x Brahman calves. This experiment followed a 4x4 latin square design with four treatments, i.e. G : 100% nature grass silage, GCF1 : 35% nature grass silage, 35% corn fodder silage and 30% concentrate, GCF2 : 17,5% nature grass silage, 52,5% corn fodder and 30% concentrate, GCF3 : 70% corn fodder and 30% concentrate. The measured variables included fiber intake and digestibility, volatile fatty acid and blood glucose concentration. Data were subjected to Analysis of Variance (Anova) followed by Duncan Multiple Range Test to separate between means with SPSS 21. Result showed that fiber intake was significantly (P<0,05) lower in GCF3 (299,88 g.d-1) compared to the other treatments which varied between 661,55 to 1293,95 g.d-1. Fiber digestibility, however, did not differ (P>0,05) between treatments, i.e. varied between 55,95 to 72,65%. Volatile Fatty Acid concentration increased (P<0,05) with the increasing levels substitutions of corn fodder in feed. The treatments did not affect (P>0,05) blood glucose concentration of Ongole calves. It can be concluded that substitution of corn fodder for native grass silage has significant effect on fiber intake and Volatile Fatty Acid concentration but not on fiber digestibility and blood glucose concentration of weaned cross Ongole calves.

Effects of dietary cation and anion difference on eating, ruminal function and plasma leptin in goats under tropical condition

Objective: This study was carried out to determine the effects of elevated dietary cation and anion difference (DCAD) on dry matter intake (DMI) and ruminal fermentation pattern in lactating dairy goats under tropical conditions.Methods: Ten dairy goats were divided into two groups of five animals each. The groups received diets at different DCAD levels, either a control diet (22.81 mEq/100 g dry matter [DM], DCAD-23) or a DCAD-39 diet (39.08 mEq/100 g DM, DCAD-39). After parturition, DMI and water intake were recorded daily. Ruminal fluid and urine were collected, and nutrient digestibility measurements were carried out at 8th weeks postpartum (PP-8). Blood samples were collected at PP-4 and PP-8 to measure plasma leptin.Results: Dry matter intake/body weight (DMI/BW) at PP-8 of the animals fed the DCAD-39 diet was significantly higher than those fed with DCAD-23 diet (p<0.05). Animals fed with DCAD-39 consumed more water than those fed DCAD-23 over 24 h, particularly at night (p<0.05). Ruminal pH, acetate concentration, and urinary allantoin excretion increased with the DCAD-39 diet, whereas ruminal butyrate concentration was lower with the DCAD-39 diet. On the other hand, other ruminal parameters, such as total volatile fatty acid concentration, propionate molar proportion and acetate/propionate average ratio, were not affected by increased DCAD supplementation. Apparent digestibility was improved by increased DCAD supplementation. Plasma leptin concentration was higher with DCAD supplementation.Conclusion: When feeding goats with DCAD-39 under tropical conditions, an increase in DMI was associated with improved apparent digestibility of nutrients, ruminal fermentation and microbial protein synthesis. An increase in plasma leptin concentration could not explain the effect of high DCAD on DMI.