Semi-automatic morphometric analysis of normal boar sperm head

2016 ◽  
Vol 169 ◽  
pp. 112 ◽  
Author(s):  
A.M. Syanda ◽  
G. Boe-Hansen ◽  
N. Satake
Keyword(s):  
Morphometric Analysis ◽  
Sperm Head ◽  
Boar Sperm

Effects of chelating agents during freeze-drying of boar spermatozoa on DNA fragmentation and on developmental ability in vitro and in vivo after intracytoplasmic sperm head injection

Zygote ◽  
2007 ◽  
Vol 15 (1) ◽  
pp. 15-24 ◽  
Author(s):  
M. Nakai ◽  
N. Kashiwazaki ◽  
A. Takizawa ◽  
N. Maedomari ◽  
M. Ozawa ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Freeze Drying ◽  
Chelating Agents ◽  
Sperm Head ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Freeze Dried ◽  
Boar Sperm

SUMMARYSuccessful offspring production after intracytoplasmic injection of freeze-dried sperm has been reported in laboratory animals but not in domesticated livestock, including pigs. The integrity of the DNA in the freeze-dried sperm is reported to affect embryogenesis. Release of endonucleases from the sperm is one of the causes of induction of sperm DNA fragmentation. We examined the effects of chelating agents, which inhibit the activation of such enzymes, on DNA fragmentation in freeze-dried sperm and on the in vitro and in vivo developmental ability of porcine oocytes following boar sperm head injection. Boar ejaculated sperm were sonicated, suspended in buffer supplemented with (1) 50 mM EGTA, (2) 50 mM EDTA, (3) 10 mM EDTA, or (4) no chelating agent and freeze-dried. A fertilization medium (Pig-FM) was used as a control. The rehydrated spermatozoa in each group were then incubated in Pig-FM at room temperature. The rate of DNA fragmentation in the control group, as assessed by the TUNEL method, increased gradually as time after rehydration elapsed (2.8% at 0 min to 12.2% at 180 min). However, the rates in all experimental groups (1–4) did not increase, even at 180 min (0.7–4.1%), which were all significantly lower (p < 0.05) than that of the control group. The rate of blastocyst formation after the injection in the control group (6.0%) was significantly lower (p < 0.05) than those in the 50 mM EGTA (23.1%) and 10 mM EDTA (22.6%) groups incubated for 120–180 min. The average number of blastocyst cells in the 50 mM EGTA group (33.1 cells) was significantly higher (p < 0.05) than that in the 10 mM EDTA group (17.8 cells). Finally, we transferred oocytes from 50 mM EGTA or control groups incubated for 0–60 min into estrous-synchronized recipients. The two recipients of the control oocytes became pregnant and one miscarried two fetuses on day 39.The results suggested that fragmentation of DNA in freeze-dried boar sperm is one of the causes of decreased in vitro developmental ability of injected oocytes to the blastocyst stage. Supplementation with EGTA in a freeze-drying buffer improves this ability.

Involvement of cAMP-dependent unique signaling cascades in the decrease of serine/threonine–phosphorylated proteins in boar sperm head

2016 ◽  
Vol 85 (6) ◽  
pp. 1152-1160 ◽  
Author(s):  
Ayane Isono ◽  
Shunsuke Tate ◽  
Kazumi Nakamura-Mori ◽  
Taichi Noda ◽  
Sho Ishikawa ◽  
...  
Keyword(s):  
Sperm Head ◽  
Signaling Cascades ◽  
Phosphorylated Proteins ◽  
Boar Sperm

scholarly journals ACRBP (Sp32) is involved in priming sperm for the acrosome reaction and the binding of sperm to the zona pellucida in a porcine model

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0251973
Author(s):  
Yoku Kato ◽  
Satheesh Kumar ◽  
Christian Lessard ◽  
Janice L. Bailey
Keyword(s):  
Endoplasmic Reticulum ◽  
Zona Pellucida ◽  
Acrosome Reaction ◽  
Porcine Model ◽  
Sperm Head ◽  
Mouse Sperm ◽  
Phosphorylated Proteins ◽  
Boar Sperm

In boar sperm, we have previously shown that capacitation is associated with the appearance of the p32 tyrosine phosphoprotein complex. The principal tyrosine phosphoprotein involved in this complex is the acrosin-binding protein (ACRBP), which regulates the autoconversion of proacrosin to intermediate forms of acrosin in both boar and mouse sperm. However, the complete biological role of ACRBP has not yet been elucidated. In this study, we tested the hypothesis that tyrosine phophorylation and the presence of the ACRBP in the sperm head are largely necessary to induce capacitation, the acrosome reaction (AR) and sperm-zona pellucida (ZP) binding, all of which are necessary steps for fertilization. In vitro fertilization (IVF) was performed using matured porcine oocytes and pre-capacitated boar sperm cultured with anti-phosphotyrosine antibodies or antibodies against ACRBP. Anti-ACRBP antibodies reduced capacitation and spontaneous AR (P<0.05). Sperm-ZP binding declined in the presence of anti-phosphotyrosine or anti-ACRBP antibodies. The localisation of anti-ACRBP antibodies on the sperm head, reduced the ability of the sperm to undergo the AR in response to solubilized ZP or by inhibiting the sarco/endoplasmic reticulum Ca2+-ATPase. These results support our hypothesis that tyrosine phosphorylated proteins and ACRBP are present upon the sperm surface in order to participate in sperm-ZP binding, and that ACRBP upon the surface of the sperm head facilitates capacitation and the AR in the porcine.

scholarly journals Topographical distribution of phospholipids in boar sperm plasma and intracellular membranes as revealed by freeze-fracture cytochemistry.

1996 ◽  
Vol 44 (7) ◽  
pp. 687-701 ◽  
Author(s):  
F W Kan ◽  
Y Lin
Keyword(s):  
Plasma Membrane ◽  
Phospholipid Composition ◽  
Sperm Head ◽  
Membrane Domains ◽  
Differential Distribution ◽  
Middle Piece ◽  
Topographical Distribution ◽  
Significant Difference ◽  
Boar Sperm ◽  
Principal Piece

We used fracture-label and label-fracture cytochemistry in conjunction with the phospholipase A2-colloidal gold (PLA2-CG) technique to study the distribution of phospholipids in ejaculated boar spermatozoa. These techniques provide visualization of the topographical distribution of phospholipids in freeze-fractured sperm membranes in a three-dimensional view. In various freeze-fractured boar sperm membranes and crossfractured cytoplasmic structures, quantitative analysis revealed that the nuclear envelope membranes and the nuclear content possessed the highest labeling density of PLA2-CG. Moderate labeling was detected over acrosomal membranes, especially the inner acrosomal membrane. Replicas of both protoplasmic and exoplasmic fracture faces of the plasma membrane of boar sperm head showed a relatively low density of PLA2-CG labeling. Moreover, a differential distribution of phospholipids was seen over the protoplasmic face of the plasma membrane domains of the sperm head, which showed the highest concentration of gold particles in the postacrosomal region, followed by the equatorial segment and the anterior acrosome region. The PLA2-CG labeling densities over the post-acrosomal region and the equatorial segment were significantly higher than that over the anterior acrosome region. In the flagellum, an intense labeling was also seen over crossfractured mitochondria, dense fibers, and fibrous sheath. The protoplasmic fracture face of the plasma membrane over the middle piece, the annulus, and the principal piece was moderately labeled by PLA2-CG. No significant difference in mean labeling density of PLA2-CG was detected among the three membrane domains. In label-fracture preparations, exoplasmic halves of the plasma membrane of the head and the middle piece of the tail were uniformly labeled with PLA2-CG. However, the annulus and principal piece were devoid of PLA2-CG binding sites. These results indicate that differential distribution of phospholipids associated with the boar sperm membranes may reflect phospholipid composition of membrane domains characteristic of special physiological functions.

Morphometry of boar sperm head and flagellum in semen backflow after insemination

2015 ◽  
Vol 84 (4) ◽  
pp. 566-574 ◽  
Author(s):  
Francisco Alberto García–Vázquez ◽  
Iván Hernández-Caravaca ◽  
Wellington Yánez-Quintana ◽  
Carmen Matás ◽  
Cristina Soriano-Úbeda ◽  
...  
Keyword(s):  
Sperm Head ◽  
Boar Sperm

scholarly journals Season of collection and sperm head shape impacts expression of CARHSP and FTL from motile-rich boar sperm

Agri Gene ◽  
2018 ◽  
Vol 7 ◽  
pp. 1-6 ◽  
Author(s):  
L.A. Rempel ◽  
M.M. Krautkramer ◽  
T.M. Loether ◽  
J.J. Parrish ◽  
J.R. Miles
Keyword(s):  
Sperm Head ◽  
Head Shape ◽  
Boar Sperm

Morphometric analysis of llama (Lama glama) sperm head

Andrologia ◽  
2011 ◽  
Vol 44 ◽  
pp. 424-430 ◽  
Author(s):  
C. Casaretto ◽  
D. M. Lombardo ◽  
S. Giuliano ◽  
M. Gambarotta ◽  
M. I. Carretero ◽  
...  
Keyword(s):  
Morphometric Analysis ◽  
Sperm Head ◽  
Lama Glama

Centrifugation on Percoll density gradient enhances motility, membrane integrity and in vitro fertilizing ability of frozen–thawed boar sperm

Zygote ◽  
2013 ◽  
Vol 23 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Michiko Noguchi ◽  
Koji Yoshioka ◽  
Hirokazu Hikono ◽  
Gentaro Iwagami ◽  
Chie Suzuki ◽  
...  
Keyword(s):  
Density Gradient ◽  
Embryo Development ◽  
Sperm Motility ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Sperm Head ◽  
Boar Sperm ◽  
Percoll Density Gradient ◽  
Motility Parameters

SummaryThe effects of Percoll density gradient centrifugation on sperm quality, in vitro fertilizability and developmental capacity of frozen–thawed boar sperm were evaluated. Two-step density gradient centrifugation by Percoll enhanced significantly the motility parameters of sperm compared with a simple centrifugation procedure. Percentages of motile sperm and sperm with intact plasma and acrosome membranes after Percoll separation were significantly greater than those after simple centrifugation. The rates of penetration, cleavage and blastocyst formation after in vitro fertilization were significantly improved by Percoll separation compared with simple centrifugation and were influenced positively by the intactness of sperm head membranes, but not any sperm motility parameters. However, insemination with increased concentrations of sperm prepared by Percoll gradient centrifugation did not improve the success of fertilization and embryo development in vitro. Our results indicate that the integrity of sperm head membranes after Percoll separation is important for successful embryo development in vitro, more so than sperm motility.

Differences in boar sperm head shape and dimensions recorded by computer-assisted sperm morphometry are not related to chromatin integrity

2007 ◽  
Vol 68 (2) ◽  
pp. 196-203 ◽  
Author(s):  
F. Saravia ◽  
I. Núñez-Martínez ◽  
J.M. Morán ◽  
C. Soler ◽  
A. Muriel ◽  
...  
Keyword(s):  
Sperm Head ◽  
Computer Assisted ◽  
Head Shape ◽  
Boar Sperm ◽  
Sperm Morphometry ◽  
Chromatin Integrity
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