Refining insulin concentrations in culture medium containing growth factors BMP15 and GDF9: An in vitro study of the effects on follicle development of goats

2017 ◽  
Vol 185 ◽  
pp. 118-127 ◽  
Author(s):  
D.J. Dipaz-Berrocal ◽  
N.A.R. Sá ◽  
D.D. Guerreiro ◽  
J.J.H. Celestino ◽  
J. Leiva-Revilla ◽  
...  
Keyword(s):  
Growth Factors ◽  
Culture Medium ◽  
In Vitro Study ◽  
Vitro Study ◽  
Follicle Development

scholarly journals Contaminação microbiológica de fios retratores: um estudo in vitro

2017 ◽  
Vol 32 (1) ◽  
pp. 47
Author(s):  
Marina Lucia Cumerlato ◽  
Darlene Ribeiro dos Santos Gerzson ◽  
Edison Maciel Nunes De Souza Filho ◽  
Everton Ribeiro Dos Santos ◽  
Marina Lucia Cumerlato ◽  
...  
Keyword(s):  
Infection Control ◽  
Bacterial Growth ◽  
Dental Care ◽  
Growth Analysis ◽  
Culture Medium ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study

OBJECTIVE: Dental care has been particularly concerned with sterilization of instruments and materials. In this context, the methods used for infection control are essential to ensure patients’ safety. This in vitro study aimed to test gingival retraction cords taken from factory- sealed containers for the presence of contamination. Three commercial brands of retraction cords were analyzed: Retraflex®, Pró-Retract®, and Ultrapack®.METHODS: The sample consisted of 10 1-cm segments of retraction cords of each commercial brand (n = 30). For bacterial growth analysis, 30 test tubes containing sterile brain heart infusion (BHI) as the culture medium were used. Bacterial growth was considered positive in tubes in which the BHI broth became turbid.RESULTS: Of 30 test tubes with retraction cords, six showed turbidity  and were considered contaminated: three tubes with Retraflex®, one tube with Pro-Retract®, and two tubes with Ultrapack®, accounting for 20% of the total sample.CONCLUSIONS: The present findings showed that some retraction cords received from the manufacturers were contaminated with microorganisms. Thus, extra caution should be taken when using these materials, and further studies should be conducted.

scholarly journals Antibacterial effect of Titanium alloys with Copper Nanoparticles: In vitro study.

2019 ◽  
Vol 5 (4) ◽  
pp. 136-140
Author(s):  
Nataly Silva-Arriagada ◽  
Andrés Gómez ◽  
Marlene Muñoz
Keyword(s):  
Titanium Alloys ◽  
Copper Nanoparticles ◽  
Culture Medium ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Vitro Study ◽  
Bacterial Strains ◽  
Lower Growth

Objective: To evaluate the in-vitro antimicrobial effect of Titanium alloys with Copper Nanoparticles (CuNP) against Streptococcus mutans and Phorphyromonas gingivalis . Materials and Methods: An in vitro study was carried out. The unit of analysis corresponded to 10 healing abutments. In 5 abutments Copper nanoparticles (CuNP) electrodeposition was applied. The remaining 5 abutments corresponded to control. The healing abutments were then immersed in culture medium for S. mutans and P. gingivalis for 14 days. Results: The agar plates with CuNP-coated abutments showed a lower growth, statistically significant for both bacterial strains. Conclusion: There is a statistically significant lower growth of S. mutans and P. gingivalis in healing abutments with CuNP.

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