scholarly journals Contaminação microbiológica de fios retratores: um estudo in vitro

2017 ◽  
Vol 32 (1) ◽  
pp. 47
Author(s):  
Marina Lucia Cumerlato ◽  
Darlene Ribeiro dos Santos Gerzson ◽  
Edison Maciel Nunes De Souza Filho ◽  
Everton Ribeiro Dos Santos ◽  
Marina Lucia Cumerlato ◽  
...  
Keyword(s):  
Infection Control ◽  
Bacterial Growth ◽  
Dental Care ◽  
Growth Analysis ◽  
Culture Medium ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study

OBJECTIVE: Dental care has been particularly concerned with sterilization of instruments and materials. In this context, the methods used for infection control are essential to ensure patients’ safety. This in vitro study aimed to test gingival retraction cords taken from factory- sealed containers for the presence of contamination. Three commercial brands of retraction cords were analyzed: Retraflex®, Pró-Retract®, and Ultrapack®.METHODS: The sample consisted of 10 1-cm segments of retraction cords of each commercial brand (n = 30). For bacterial growth analysis, 30 test tubes containing sterile brain heart infusion (BHI) as the culture medium were used. Bacterial growth was considered positive in tubes in which the BHI broth became turbid.RESULTS: Of 30 test tubes with retraction cords, six showed turbidity  and were considered contaminated: three tubes with Retraflex®, one tube with Pro-Retract®, and two tubes with Ultrapack®, accounting for 20% of the total sample.CONCLUSIONS: The present findings showed that some retraction cords received from the manufacturers were contaminated with microorganisms. Thus, extra caution should be taken when using these materials, and further studies should be conducted.

An In Vitro Analysis of Sodium Hypochlorite Decontamination for the Reuse of Implant Healing Abutments

2020 ◽  
Author(s):  
Ahmad Almehmadi
Keyword(s):  
Sodium Hypochlorite ◽  
Bacterial Culture ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Vitro Study ◽  
Sem Images ◽  
Streptococcus Sanguis ◽  
Implant Dentistry ◽  
Vitro Analysis

Abstract The re-use of healing abutments (HAs) has become common practice in implant dentistry for economic concerns and the aim of this in-vitro study was to assess the effect of sodium hypochlorite (NaOCl) in decontamination of HAs. 122 HAs (Used and sterilized n=107; New n=15) were procured from 3 centers, of which 3 samples were discarded due to perforation in sterilization pouch.  For sterility assessment, the used HAs (n=80) were cultured in Brain Heart Infusion Broth (BHI) and Potato Dextrose Agar (PDA), bacterial isolates were identified in 7 samples. Also, 24 used HAs were stained with Phloxine B, photographed and compared to new HAs (n=5). Scanning electron microscope (SEM) assessed the differences between the two sets of HAs, following which the 7 contaminated HAs along with 24 used HAs from staining experiment (Total=31) were subsequently treated with sodium hypochlorite (NaOCl) and SEM images were observed. About 8.75% of HAs tested positive in bacterial culture; Streptococcus sanguis, Dermabacter hominis, Staphylococcus haemolyticus, and Aspergillus species were isolated. Phloxine B staining was positive for used and sterilized HAs when compared to controls. The SEM images revealed deposits in the used HAs and although treatment with NaOCl eliminated the contamination of cultured HAs, the SEM showed visible debris in the HA thread region. This in-vitro study concluded that SEM images showed debris in used HAs at screw-hole and thread regions even though they tested negative in bacterial culture. The treatment with NaOCl of used HAs showed no bacterial contamination but the debris was observed in SEM images. Future studies on the chemical composition, biological implications, and clinical influence is warranted before considering the reuse of HAs.

Refining insulin concentrations in culture medium containing growth factors BMP15 and GDF9: An in vitro study of the effects on follicle development of goats

2017 ◽  
Vol 185 ◽  
pp. 118-127 ◽  
Author(s):  
D.J. Dipaz-Berrocal ◽  
N.A.R. Sá ◽  
D.D. Guerreiro ◽  
J.J.H. Celestino ◽  
J. Leiva-Revilla ◽  
...  
Keyword(s):  
Growth Factors ◽  
Culture Medium ◽  
In Vitro Study ◽  
Vitro Study ◽  
Follicle Development

scholarly journals Incidence and Pattern of Bacterial Growth in Propofol Vial - An In vitro Study

2016 ◽  
Vol 06 (02) ◽  
pp. 051-056
Author(s):  
U. S. Raveendra ◽  
Rashmi Soori ◽  
Vimal Kumar Karnekar ◽  
Swathi N. Nayak ◽  
Anand Bangera
Keyword(s):  
Bacterial Growth ◽  
Pearson Correlation ◽  
Brain Heart Infusion ◽  
In Vitro Study ◽  
Surgical Site Infections ◽  
Blood Stream ◽  
Categorical Variables ◽  
Bacillus Species ◽  
Febrile Episodes

Abstract Introduction- Propofol vials are often used in parts or are opened and left unattended. This has lead to blood stream infections, surgical site infections and acute febrile episodes. A prospective observational study was undertaken to know the incidence and pattern of bacterial growth in samples of Propofol in tropical climate. Materials and methods- Samples were collected from vials of propofol of different brands, both with and without edetate at different time intervals with relation to room temperature. Each sample of 1ml were inoculated in Brain Heart Infusion (BHI) and incubated for 48hours. Presence of bacterial growth and their pattern were studied. Statistical analysis used- Paired t test for categorical variables and for non categorical variables Levine's test and Pearson correlation. Results- Overall 42.26% of samples showed bacterial growth. The incidence was more in samples of propofol without edetate (43.75%) compared to samples with edetate (41.97 %). Most common organism was Staphylococcus aureus, followed by Enterococcus, Acinetobacter, Bacillus species, Pseudomonas and Staphylococcus citrus. Conclusion- Propofol vial once opened favours bacterial colonisation and growth. Adding edetate to propofol has not shown much benefit in decreasing the incidence.

scholarly journals Antibacterial effect of Titanium alloys with Copper Nanoparticles: In vitro study.

2019 ◽  
Vol 5 (4) ◽  
pp. 136-140
Author(s):  
Nataly Silva-Arriagada ◽  
Andrés Gómez ◽  
Marlene Muñoz
Keyword(s):  
Titanium Alloys ◽  
Copper Nanoparticles ◽  
Culture Medium ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Vitro Study ◽  
Bacterial Strains ◽  
Lower Growth

Objective: To evaluate the in-vitro antimicrobial effect of Titanium alloys with Copper Nanoparticles (CuNP) against Streptococcus mutans and Phorphyromonas gingivalis . Materials and Methods: An in vitro study was carried out. The unit of analysis corresponded to 10 healing abutments. In 5 abutments Copper nanoparticles (CuNP) electrodeposition was applied. The remaining 5 abutments corresponded to control. The healing abutments were then immersed in culture medium for S. mutans and P. gingivalis for 14 days. Results: The agar plates with CuNP-coated abutments showed a lower growth, statistically significant for both bacterial strains. Conclusion: There is a statistically significant lower growth of S. mutans and P. gingivalis in healing abutments with CuNP.

scholarly journals Evaluation of effect of different disposable infection control barriers on light intensity of light-curing unit and microhardness of composite - An in vitro study

2017 ◽  
Vol 20 (3) ◽  
pp. 180 ◽  
Author(s):  
RajivTarachand Khode ◽  
PratimaRamakrishna Shenoi ◽  
RajeshR Kubde ◽  
ChetanaS Makade ◽  
KanchanD Wadekar ◽  
...  
Keyword(s):  
Light Intensity ◽  
Infection Control ◽  
In Vitro Study ◽  
Vitro Study ◽  
Light Curing
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