Osteoinductive Activity of DFDBA Materials versus Growth Factors on Gene Expression of MG-63 Cells: An In Vitro Study

2016 ◽  
Vol 26 (2) ◽  
pp. 133-142 ◽  
Author(s):  
Surena Vahabi ◽  
Maryam Torshabi ◽  
Mohammad Mohammadi
Keyword(s):  
Gene Expression ◽  
Growth Factors ◽  
In Vitro Study ◽  
Vitro Study

An in-vitro study investigating the effect of air-abrasion bioactive glasses on dental adhesion, cytotoxicity and odontogenic gene expression

2021 ◽  
Author(s):  
Gianrico Spagnuolo ◽  
Paula Maciel Pires ◽  
Anna Calarco ◽  
Gianfranco Peluso ◽  
Avijit Banerjee ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Study ◽  
Vitro Study ◽  
Air Abrasion ◽  
Bioactive Glasses

Refining insulin concentrations in culture medium containing growth factors BMP15 and GDF9: An in vitro study of the effects on follicle development of goats

2017 ◽  
Vol 185 ◽  
pp. 118-127 ◽  
Author(s):  
D.J. Dipaz-Berrocal ◽  
N.A.R. Sá ◽  
D.D. Guerreiro ◽  
J.J.H. Celestino ◽  
J. Leiva-Revilla ◽  
...  
Keyword(s):  
Growth Factors ◽  
Culture Medium ◽  
In Vitro Study ◽  
Vitro Study ◽  
Follicle Development

scholarly journals The influence of lovastatin on thrombomodulin gene expression in vascular endothelial cells--in vitro study.

2009 ◽  
Vol 47 (1) ◽  
Author(s):  
Krzysztof Góralczyk ◽  
Krystyna Soszyńska ◽  
Olga Haus ◽  
Robert Bielis ◽  
Danuta Rość
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Vascular Endothelial

scholarly journals Effects of Berries on High-Fat Diet-Induced Inflammation

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 449-449
Author(s):  
Patricia Perez ◽  
Desiree Wanders ◽  
Hannah Land ◽  
Kathryn Chiang ◽  
Rami Najjar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Inflammatory Markers ◽  
In Vitro Study ◽  
Vitro Study ◽  
In Vivo Study ◽  
High Fat ◽  
Anti Inflammatory

Abstract Objectives Studies suggest that inflammation mediates the link between obesity and its comorbidities including type 2 diabetes and cardiovascular disease. Hence, there is a demand for effective alternative or complementary approaches to treat obesity-associated inflammation. The objective of this study was to determine whether consumption of blackberries (BL) and raspberries (RB) alone or in combination reduce obesity-induced inflammation. Methods In Vitro Study: RAW 264.7 macrophages were pretreated with either BL, RB, or BL + RB, each at a final concentration of 200 µg/mL for 2 h. LPS (1 ng/mL) was then added to the media for 16 h. mRNA expression of inflammatory cytokines was measured. In Vivo Study: Five-week-old mice were acclimated to a low-fat low-sucrose (LFLS) diet for one week after which mice were randomized 10 per group to one of five groups: 1) LFLS, 2) high-fat high-sucrose (HFHS), 3) HFHS + 10% BL, 4) HFHS + 10% RB, or 5) HFHS + 5% BL + 5% RB. Expression of inflammatory markers was measured in the liver as well as epididymal and inguinal white adipose tissue. Results In Vitro Study: Each berry alone and in combination suppressed the LPS-induced increase in inflammatory markers, with the combination (BL + RB) having the greatest effect. The combination suppressed LPS-induced expression of Ccl2, Tnfa, F4/80, and Il6 by 3.7−, 5.3−, 5.3−, and 4.4-fold, respectively. In Vivo Study: Gene expression analysis indicated that berry consumption had no significant effect on proinflammatory (Ccl2, Il1b, Tnfa, Il6, Itgam) or anti-inflammatory (Adipoq, Arg1, Mgl1) markers in adipose tissue depots or liver. However, relatively low gene expression of inflammatory markers in the tissues indicates that the mice fed the HFHS diet failed to develop a robust inflammatory state. Conclusions BL and RB have direct anti-inflammatory effects on immune cells. Initial analysis indicates that consumption of BL and RB has no significant effects on markers of inflammation in a diet-induced mouse model of obesity. However, it is possible that the relatively low levels of inflammation in these mice masked the anti-inflammatory potential of BL and RB. Ongoing analysis will provide additional insights into the effects of BL and RB on inflammation in these tissues. Funding Sources Lewis Foundation Award.

Effect of leptin and photoperiod on pituitary SOCS-3 gene expression in sheep – in vitro study

2013 ◽  
Vol 13 ◽  
pp. 15-16
Author(s):  
Małgorzata Szczęsna ◽  
Katarzyna Kirsz ◽  
Mirosław Kucharski
Keyword(s):  
Gene Expression ◽  
In Vitro Study ◽  
Vitro Study
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