Transcriptome analysis of the brain of the Chinese mitten crab, Eriocheir sinensis, for neuropeptide abundance profiles during ovarian development

2019 ◽  
Vol 201 ◽  
pp. 63-70 ◽  
Author(s):  
Xue Liu ◽  
KeYi Ma ◽  
ZhiQiang Liu ◽  
JianBin Feng ◽  
BaoQing Ye ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Ovarian Development ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
The Brain

Hepatopancreas transcriptome analysis of Chinese mitten crab (Eriocheir sinensis) with white hepatopancreas syndrome

2017 ◽  
Vol 70 ◽  
pp. 302-307 ◽  
Author(s):  
Xiaowen Chen ◽  
Jun Wang ◽  
Wucheng Yue ◽  
Jinsheng Liu ◽  
Chenghui Wang
Keyword(s):  
Transcriptome Analysis ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab

scholarly journals Evidences for Red Pigment Concentrating Hormone (RPCH) and Beta-Pigment Dispersing Hormone (β-PDH) Inducing Oocyte Meiotic Maturation in the Chinese Mitten Crab, Eriocheir sinensis

2021 ◽  
Vol 12 ◽  
Author(s):  
Ling-Ling Wei ◽  
Tian-Tian Chen ◽  
Bi-Yun Luo ◽  
Gao-Feng Qiu
Keyword(s):  
Eriocheir Sinensis ◽  
Meiotic Maturation ◽  
Oocyte Diameter ◽  
Red Pigment ◽  
Chinese Mitten Crab ◽  
Adipokinetic Hormone ◽  
Germinal Vesicle Breakdown ◽  
Mitten Crab ◽  
Oocyte Meiotic Maturation ◽  
The Brain

Red pigment concentrating hormone (RPCH) and pigment dispersing hormone (PDH) are crustacean neuropeptides involved in broad physiological processes including body color changes, circadian rhythm, and ovarian growth. In this study, the full-length cDNA of RPCH and PDH were identified from the brain of the Chinese mitten crab Eriocheir sinensis. The deduced RPCH and PDH mature peptides shared identical sequence to the adipokinetic hormone/RPCH peptides family and the β-PDH isoforms and were designated as Es-RPCH and Es-β-PDH, respectively. Es-RPCH and Es-β-PDH transcripts were distributed in the brain and eyestalks. The positive signals of Es-RPCH and Es-β-PDH were localized in the neuronal clusters 6, 8, 9, 10, and 17 of the brain as revealed by in situ hybridization. The expression level of Es-RPCH and Es-β-PDH mRNA in nervous tissues were all significantly increased at vitellogenic stage, and then decreased at the final meiotic maturation stage. The administrated with synthesized Es-RPCH peptide results in germinal vesicles shift toward the plasma membrane in vitellogenic oocyte, and significant decrease of the gonad-somatic index (GSI) and mean oocyte diameter as well as the expression of vitellogenin mRNA at 30 days post injection in vivo. Similar results were also found when injection of the Es-β-PDH peptide. In vitro culture demonstrated that Es-RPCH and Es-β-PDH induced germinal vesicle breakdown of the late vitellogenic oocytes. Comparative ovarian transcriptome analysis indicated that some reproduction/meiosis-related genes such as cdc2 kinase, cyclin B, 5-HT-R and retinoid-X receptor were significantly upregulated in response to Es-RPCH and Es-β-PDH treatments. Taken together, these results provided the evidence for the inductive effect of Es-RPCH and Es-β-PDH on the oocyte meiotic maturation in E. sinensis.

Effects of fattening period on ovarian development and nutritional quality of adult female Chinese mitten crab Eriocheir sinensis

Aquaculture ◽  
2020 ◽  
Vol 519 ◽  
pp. 734748
Author(s):  
Xiaowen Long ◽  
Qing Guo ◽  
Xichang Wang ◽  
David S. Francis ◽  
Yongxu Cheng ◽  
...  
Keyword(s):  
Adult Female ◽  
Nutritional Quality ◽  
Ovarian Development ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Fattening Period

The ovarian development pattern of pond-reared Chinese mitten crab, Eriocheir sinensis H. Milne-Edwards, 1853

Crustaceana ◽  
2017 ◽  
Vol 90 (4) ◽  
pp. 449-470 ◽  
Author(s):  
Xugan Wu ◽  
Meimei Liu ◽  
Jie Pan ◽  
Hao Chen ◽  
Chaoshu Zeng ◽  
...  
Keyword(s):  
Ovarian Development ◽  
Gonadosomatic Index ◽  
Eriocheir Sinensis ◽  
Stage Iii ◽  
Development Pattern ◽  
Hepatosomatic Index ◽  
Ovarian Maturation ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Ovarian Morphology

Although pond culture is the major culture method for Chinese mitten crab (Eriocheir sinensis) in China, the pattern of ovarian development in pond-rearedE. sinensisremains unclear. This study investigated the changes in ovarian morphology and histology, gonadosomatic index (GSI), hepatosomatic index (HSI), and monthly variation of the ovarian development pattern during the ovarian maturation of pond-reared femaleE. sinensis. Based on the pubertal moult, and ovarian morphology and histology, the ovarian development cycle ofE. sinensiscould be divided into five stages, i.e., Stage I: the ovary appears thin ribbon-like and translucent, dominated by oogonia (OG) and previtellogenic oocytes (PRO); Stage II: the ovary appears milk white or buff, dominated by endogenous vitellogenic oocytes (EN); Stage III: the ovary appears orange or light brown, dominated by exogenous vitellogenic oocytes (EX); Stage IV: the ovary appears crimson red or brown and ovarian lobes occupy most available body cavities. The major oocytes were nearly mature oocytes (NO); Stage V: the ovary appears deep purple and is filled with mature oocytes (MO). During ovarian maturation, the GSI increased significantly and a significant, positive correlation was found between the GSI and the mean long diameter of the oocyte from stage III to stage V. However, a significantly negative correlation was found between GSI and HSI. The ovarian development of pond-reared femaleE. sinensiswas not synchronous, most of the pubertal moults were found until mid-late August. At the end of August, the percentages of females that reached ovarian stages II and III were 55 and 24%, respectively. After that, the ovarian development of pond-reared femaleE. sinensisbecame fast, and females with stage III or more advanced ovaries were found to be in excess of 80% by the end of September and onward. From the end of November to late December, the GSI of femaleE. sinensisdid not increase significantly, which indicated pond-reared females had reached mature or nearly-mature ovaries by the end of November.

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