Novel, anionic, antiviral septapeptides from mosquito cells also protect monkey cells against dengue virus

2013 ◽  
Vol 98 (3) ◽  
pp. 449-456 ◽  
Author(s):  
Chaowanee Laosutthipong ◽  
Nipaporn Kanthong ◽  
Timothy W. Flegel
Keyword(s):  
Dengue Virus ◽  
Mosquito Cells

scholarly journals Mosquito metabolomics reveal that dengue virus replication requires phospholipid reconfiguration via the remodeling cycle

2020 ◽  
Vol 117 (44) ◽  
pp. 27627-27636
Author(s):  
Thomas Vial ◽  
Wei-Lian Tan ◽  
Eric Deharo ◽  
Dorothée Missé ◽  
Guillaume Marti ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Viral Genome ◽  
De Novo ◽  
Genome Replication ◽  
Mosquito Vector ◽  
Phospholipid Biosynthesis ◽  
Replication Complex ◽  
Mosquito Cells ◽  
Viral Genome Replication ◽  
Blood Meals

Dengue virus (DENV) subdues cell membranes for its cellular cycle by reconfiguring phospholipids in humans and mosquitoes. Here, we determined how and why DENV reconfigures phospholipids in the mosquito vector. By inhibiting and activating the de novo phospholipid biosynthesis, we demonstrated the antiviral impact of de novo–produced phospholipids. In line with the virus hijacking lipids for its benefit, metabolomics analyses indicated that DENV actively inhibited the de novo phospholipid pathway and instead triggered phospholipid remodeling. We demonstrated the early induction of remodeling during infection by using isotope tracing in mosquito cells. We then confirmed in mosquitoes the antiviral impact of de novo phospholipids by supplementing infectious blood meals with a de novo phospholipid precursor. Eventually, we determined that phospholipid reconfiguration was required for viral genome replication but not for the other steps of the virus cellular cycle. Overall, we now propose that DENV reconfigures phospholipids through the remodeling cycle to modify the endomembrane and facilitate formation of the replication complex. Furthermore, our study identified de novo phospholipid precursor as a blood determinant of DENV human-to-mosquito transmission.

scholarly journals Cell-Fusing Agent Virus Reduces Arbovirus Dissemination in Aedes aegypti Mosquitoes In Vivo

2019 ◽  
Vol 93 (18) ◽  
Author(s):  
Artem Baidaliuk ◽  
Elliott F. Miot ◽  
Sebastian Lequime ◽  
Isabelle Moltini-Conclois ◽  
Fanny Delaigue ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Cell Line ◽  
Content Type ◽  
Cells In Culture ◽  
Mosquito Cells ◽  
Wide Range ◽  
Natural Hosts

ABSTRACT Aedes aegypti mosquitoes are the main vectors of arthropod-borne viruses (arboviruses) of public health significance, such as the flaviviruses dengue virus (DENV) and Zika virus (ZIKV). Mosquitoes are also the natural hosts of a wide range of viruses that are insect specific, raising the question of their influence on arbovirus transmission in nature. Cell-fusing agent virus (CFAV) was the first described insect-specific flavivirus, initially discovered in an A. aegypti cell line and subsequently detected in natural A. aegypti populations. It was recently shown that DENV and the CFAV strain isolated from the A. aegypti cell line have mutually beneficial interactions in mosquito cells in culture. However, whether natural strains of CFAV and DENV interact in live mosquitoes is unknown. Using a wild-type CFAV isolate recently derived from Thai A. aegypti mosquitoes, we found that CFAV negatively interferes with both DENV type 1 and ZIKV in vitro and in vivo. For both arboviruses, prior infection by CFAV reduced the dissemination titer in mosquito head tissues. Our results indicate that the interactions observed between arboviruses and the CFAV strain derived from the cell line might not be a relevant model of the viral interference that we observed in vivo. Overall, our study supports the hypothesis that insect-specific flaviviruses may contribute to reduce the transmission of human-pathogenic flaviviruses. IMPORTANCE The mosquito Aedes aegypti carries several arthropod-borne viruses (arboviruses) that are pathogenic to humans, including dengue and Zika viruses. Interestingly, A. aegypti is also naturally infected with insect-only viruses, such as cell-fusing agent virus. Although interactions between cell-fusing agent virus and dengue virus have been documented in mosquito cells in culture, whether wild strains of cell-fusing agent virus interfere with arbovirus transmission by live mosquitoes was unknown. We used an experimental approach to demonstrate that cell-fusing agent virus infection reduces the propagation of dengue and Zika viruses in A. aegypti mosquitoes. These results support the idea that insect-only viruses in nature can modulate the ability of mosquitoes to carry arboviruses of medical significance and that they could possibly be manipulated to reduce arbovirus transmission.

Isolation and characterization of exosomes released from mosquito cells infected with dengue virus

2019 ◽  
Vol 266 ◽  
pp. 1-14 ◽  
Author(s):  
José Manuel Reyes-Ruiz ◽  
Juan Fidel Osuna-Ramos ◽  
Luis Adrián De Jesús-González ◽  
Arianna Mahely Hurtado-Monzón ◽  
Carlos Noe Farfan-Morales ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Isolation And Characterization ◽  
Mosquito Cells

scholarly journals Inhibitory effect of small interfering RNA on dengue virus replication in mosquito cells

2010 ◽  
Vol 7 (1) ◽  
pp. 270 ◽  
Author(s):  
Xinwei Wu ◽  
Hua Hong ◽  
Jinya Yue ◽  
Yejian Wu ◽  
Xiangzhong Li ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Replication ◽  
Small Interfering Rna ◽  
Inhibitory Effect ◽  
Mosquito Cells ◽  
Interfering Rna

scholarly journals Strand-like structures and the nonstructural proteins 5, 3 and 1 are present in the nucleus of mosquito cells infected with dengue virus

Virology ◽  
2018 ◽  
Vol 515 ◽  
pp. 74-80 ◽  
Author(s):  
José M. Reyes-Ruiz ◽  
Juan F. Osuna-Ramos ◽  
Margot Cervantes-Salazar ◽  
Anel E. Lagunes Guillen ◽  
Bibiana Chávez-Munguía ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Nonstructural Proteins ◽  
Mosquito Cells

scholarly journals A novel p53 paralogue mediates antioxidant defense of mosquito cells to survive dengue virus replication

Virology ◽  
2018 ◽  
Vol 519 ◽  
pp. 156-169 ◽  
Author(s):  
Tien-Huang Chen ◽  
Yi-Jun Wu ◽  
Jiun-Nan Hou ◽  
Yi-Hsuan Chiang ◽  
Chih-Chieh Cheng ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Replication ◽  
Antioxidant Defense ◽  
Mosquito Cells

scholarly journals The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex

2018 ◽  
Vol 93 (4) ◽  
Author(s):  
Romel Rosales Ramirez ◽  
Juan E. Ludert
Keyword(s):  
Dengue Virus ◽  
Nonstructural Protein ◽  
Cell Interactions ◽  
Vertebrate Host ◽  
Mosquito Vector ◽  
Caveolin 1 ◽  
Nonstructural Protein 1 ◽  
Virion Release ◽  
Mosquito Cells ◽  
Infected Cells

ABSTRACTDengue virus (DENV) is a mosquito-borne virus of the familyFlaviviridae. The RNA viral genome encodes three structural and seven nonstructural proteins. Nonstructural protein 1 (NS1) is a multifunctional protein actively secreted in vertebrate and mosquito cells during infection. In mosquito cells, NS1 is secreted in a caveolin-1-dependent manner by an unconventional route. The caveolin chaperone complex (CCC) is a cytoplasmic complex formed by caveolin-1 and the chaperones FKBP52, Cy40, and CyA and is responsible for the cholesterol traffic inside the cell. In this work, we demonstrate that in mosquito cells, but not in vertebrate cells, NS1 associates with and relies on the CCC for secretion. Treatment of mosquito cells with classic secretion inhibitors, such as brefeldin A, Golgicide A, and Fli-06, showed no effect on NS1 secretion but significant reductions in recombinant luciferase secretion and virion release. Silencing the expression of CAV-1 or FKBP52 with short interfering RNAs or the inhibition of CyA by cyclosporine resulted in significant decrease in NS1 secretion, again without affecting virion release. Colocalization, coimmunoprecipitation, and proximity ligation assays indicated that NS1 colocalizes and interacts with all proteins of the CCC. In addition, CAV-1 and FKBP52 expression was found augmented in DENV-infected cells. Results obtained with Zika virus-infected cells suggest that in mosquito cells, ZIKV NS1 follows the same secretory pathway as that observed for DENV NS1. These results uncover important differences in the dengue virus-cell interactions between the vertebrate host and the mosquito vector as well as novel functions for the chaperone caveolin complex.IMPORTANCEThe dengue virus protein NS1 is secreted efficiently from both infected vertebrate and mosquito cells. Previously, our group reported that NS1 secretion in mosquito cells follows an unconventional secretion pathway dependent on caveolin-1. In this work, we demonstrate that in mosquito cells, but not in vertebrate cells, NS1 secretion takes place in association with the chaperone caveolin complex, a complex formed by caveolin-1 and the chaperones FKBP52, CyA, and Cy40, which are in charge of cholesterol transport inside the cell. Results obtained with ZIKV-infected mosquito cells suggest that ZIKV NS1 is released following an unconventional secretory route in association with the chaperone caveolin complex. These results uncover important differences in the virus-cell interactions between the vertebrate host and the mosquito vector, as well as novel functions for the chaperone caveolin complex. Moreover, manipulation of the NS1 secretory route may prove a valuable strategy to combat these two mosquito-borne diseases.

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