Recombinant pseudorabies virus expressing E2 of classical swine fever virus (CSFV) protects against both virulent pseudorabies virus and CSFV

ABSTRACTClassical swine fever (CSF) is an economically important infectious disease of pigs caused by classical swine fever virus (CSFV). Pseudorabies (PR), which is caused by pseudorabies virus (PRV), is another important infectious disease of pigs and other animals. Coinfections of pigs with PRV and CSFV occur occasionally in the field. The modified live vaccine Bartha-K61 strain has played an important role in the control of PR in many countries, including China. Since late 2011, however, increasing PR outbreaks caused by an emerging PRV variant have been reported in Bartha-K61-vaccinated swine populations on many farms in China. Previously, we generated a gE/gI-deleted PRV (rPRVTJ-delgE) based on this PRV variant, which was shown to be safe and can provide rapid and complete protection against lethal challenge with the PRV variant in pigs. Here, we generated a new recombinant PRV variant expressing the E2 gene of CSFV (rPRVTJ-delgE/gI-E2) and evaluated its immunogenicity and efficacy in pigs. The results showed that rPRVTJ-delgE/gI-E2 was safe for pigs, induced detectable anti-PRV and anti-CSFV neutralizing antibodies, and provided complete protection against the lethal challenge with either the PRV TJ strain or the CSFV Shimen strain. The data indicate that rPRVTJ-delgE/gI-E2 is a promising candidate bivalent vaccine against PRV and CSFV coinfections.

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Stability of classical swine fever virus and pseudorabies virus in animal feed ingredients exposed to transpacific shipping conditions

Transboundary and Emerging Diseases ◽

10.1111/tbed.13498 ◽

2020 ◽

Vol 67 (4) ◽

pp. 1623-1632 ◽

Cited By ~ 1

Author(s):

Ana M. M. Stoian ◽

Vlad Petrovan ◽

Laura A. Constance ◽

Matthew Olcha ◽

Scott Dee ◽

...

Keyword(s):

Classical Swine Fever Virus ◽

Pseudorabies Virus ◽

Animal Feed ◽

Classical Swine Fever ◽

Fever Virus ◽

Feed Ingredients

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Generation and Immunogenicity of a Recombinant Pseudorabies Virus Co-Expressing Classical Swine Fever Virus E2 Protein and Porcine Circovirus Type 2 Capsid Protein Based on Fosmid Library Platform

Pathogens ◽

10.3390/pathogens8040279 ◽

2019 ◽

Vol 8 (4) ◽

pp. 279

Author(s):

Muhammad Abid ◽

Teshale Teklue ◽

Yongfeng Li ◽

Hongxia Wu ◽

Tao Wang ◽

...

Keyword(s):

Classical Swine Fever Virus ◽

Pseudorabies Virus ◽

Classical Swine Fever ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Fosmid Library ◽

Fever Virus ◽

Porcine Circovirus ◽

Live Virus

Pseudorabies (PR), classical swine fever (CSF), and porcine circovirus type 2 (PCV2)-associated disease (PCVAD) are economically important infectious diseases of pigs. Co-infections of these diseases often occur in the field, posing significant threat to the swine industry worldwide. gE/gI/TK-gene-deleted vaccines are safe and capable of providing full protection against PR. Classical swine fever virus (CSFV) E2 glycoprotein is mainly used in the development of CSF vaccines. PCV2 capsid (Cap) protein is the major antigen targeted for developing PCV2 subunit vaccines. Multivalent vaccines, and especially virus-vectored vaccines expressing foreign proteins, are attractive strategies to fight co-infections for various swine diseases. The gene-deleted pseudorabies virus (PRV) can be used to develop promising and economical multivalent live virus-vectored vaccines. Herein, we constructed a gE/gI/TK-gene-deleted PRV co-expressing E2 of CSFV and Cap of PCV2 by fosmid library platform established for PRV, and the expression of E2 and Cap proteins was confirmed using immunofluorescence assay and western blotting. The recombinant virus propagated in porcine kidney 15 (PK-15) cells for 20 passages was genetically stable. The evaluation results in rabbits and pigs demonstrate that rPRVTJ-delgE/gI/TK-E2-Cap elicited detectable anti-PRV antibodies, but not anti-PCV2 or anti-CSFV antibodies. These findings provide insights that rPRVTJ-delgE/gI/TK-E2-Cap needs to be optimally engineered as a promising trivalent vaccine candidate against PRV, PCV2 and CSFV co-infections in future.

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Safety and immunogenicity of a gE/gI/TK gene-deleted pseudorabies virus variant expressing the E2 protein of classical swine fever virus in pigs

Immunology Letters ◽

10.1016/j.imlet.2016.04.014 ◽

2016 ◽

Vol 174 ◽

pp. 63-71 ◽

Cited By ~ 9

Author(s):

Jian-Lin Lei ◽

Shui-Li Xia ◽

Yimin Wang ◽

Mingliang Du ◽

Guang-Tao Xiang ◽

...

Keyword(s):

Classical Swine Fever Virus ◽

Pseudorabies Virus ◽

Classical Swine Fever ◽

Fever Virus ◽

Virus Variant ◽

E2 Protein

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ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells

Veterinary Microbiology ◽

10.1016/j.vetmic.2021.109034 ◽

2021 ◽

Vol 255 ◽

pp. 109034

Author(s):

Liang Zhang ◽

Mingxing Jin ◽

Mengzhao Song ◽

Shanchuan Liu ◽

Tao Wang ◽

...

Keyword(s):

Classical Swine Fever Virus ◽

Classical Swine Fever ◽

Fever Virus ◽

Ns5a Protein

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Genome Variability of Classical Swine Fever Virus during the 2018–2020 Epidemic in Japan

Veterinary Microbiology ◽

10.1016/j.vetmic.2021.109128 ◽

2021 ◽

pp. 109128

Author(s):

Tatsuya Nishi ◽

Katsuhiko Fukai ◽

Tomoko Kato ◽

Kotaro Sawai ◽

Takehisa Yamamoto

Keyword(s):

Classical Swine Fever Virus ◽

Classical Swine Fever ◽

Fever Virus ◽

Genome Variability

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A Novel E2 Glycoprotein Subunit Marker Vaccine Produced in Plant Is Able to Prevent Classical Swine Fever Virus Vertical Transmission after Double Vaccination

Vaccines ◽

10.3390/vaccines9050418 ◽

2021 ◽

Vol 9 (5) ◽

pp. 418

Author(s):

Youngmin Park ◽

Yeonsu Oh ◽

Miaomiao Wang ◽

Llilianne Ganges ◽

José Alejandro Bohórquez ◽

...

Keyword(s):

Vertical Transmission ◽

Classical Swine Fever Virus ◽

Neutralizing Antibodies ◽

Subunit Vaccine ◽

Vaccine Dose ◽

Classical Swine Fever ◽

Fever Virus ◽

Tissue Samples ◽

Marker Vaccine ◽

E2 Glycoprotein

The efficacy of a novel subunit vaccine candidate, based in the CSFV E2 glycoprotein produced in plants to prevent classical swine fever virus (CSFV) vertical transmission, was evaluated. A Nicotiana benthamiana tissue culture system was used to obtain a stable production of the E2-glycoprotein fused to the porcine Fc region of IgG. Ten pregnant sows were divided into three groups: Groups 1 and 2 (four sows each) were vaccinated with either 100 μg/dose or 300 μg/dose of the subunit vaccine at 64 days of pregnancy. Group 3 (two sows) was injected with PBS. Groups 1 and 2 were boosted with the same vaccine dose. At 10 days post second vaccination, the sows in Groups 2 and 3 were challenged with a highly virulent CSFV strain. The vaccinated sows remained clinically healthy and seroconverted rapidly, showing efficient neutralizing antibodies. The fetuses from vaccinated sows did not show gross lesions, and all analyzed tissue samples tested negative for CSFV replication. However, fetuses of non-vaccinated sows had high CSFV replication in tested tissue samples. The results suggested that in vaccinated sows, the plant produced E2 marker vaccine induced the protective immunogenicity at challenge, leading to protection from vertical transmission to fetuses.

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