Impact Evaluation of Marking Salmo trutta with Alizarin Red S Produced by Different Manufacturers

2021 ◽  
pp. 106051
Author(s):  
Živilė Jurgelėnė ◽  
Danguolė Montvydienė ◽  
Saulius Stakėnas ◽  
Justas Poviliūnas ◽  
Simonas Račkauskas ◽  
...  
Keyword(s):  
Impact Evaluation ◽  
Salmo Trutta ◽  
Alizarin Red S ◽  
Alizarin Red

scholarly journals Globular adiponectin inhibits osteoblastic differentiation of vascular smooth muscle cells through the PI3K/AKT and Wnt/β-catenin pathway

2021 ◽  
Author(s):  
Yun Zhou ◽  
Li-Long Wei ◽  
Rui-Ping Zhang ◽  
Cheng-Wu Han ◽  
Yongtong Cao
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Osteoblastic Differentiation ◽  
Alizarin Red S ◽  
Regulation Mechanism ◽  
Alizarin Red ◽  
Globular Adiponectin

AbstractLipid metabolism is closely related to the improvement of vascular calcification (VC) in chronic kidney disease (CKD). Globular adiponectin (gAd) has been reported to be involved in the development of VC in CKD, but the detailed regulatory role remains unclear. The present study is aimed to investigate the biological function and the underlying regulation mechanism of gAd in the process of VC during CKD. Vascular smooth muscle cells (VSMCs) calcification was determined by Alizarin Red S staining. Protein signaling related with VC was tested by western blotting. The expression and intracellular localization of runt-related transcription factor 2 (Runx2) was detected by immunofluorescence and uraemic rat with VC was established by a two-step nephrectomy. Combined with the results of Alizarin Red S staining, we discovered that β-glycerophosphate (β-Gp)-induced the osteoblastic differentiation of VSMCs was significantly reversed by gAd treatment. Along with the VSMCs calcification and the increase of Runx2 in β-Gp-exposed VSMCs, the activities of protein kinase B (AKT) and Wnt/β-catenin pathway were enhanced, but that were counteracted by the exposure of gAd in rat and human VSMCs. After administration with agonists of the Wnt (SKL2001) and AKT (SC79), there appeared more osteoblastic differentiation and higher expression of Runx2 in gAd-treated VSMCs, but showing lower impact in the presence of SC79 than that in the presence of SKL2001. In the in vivo experiments, intravenous injection of gAd also significantly inhibited VC and Runx2 level in uraemic rat in a dose-dependent manner, possibly through regulating Wnt/β-catenin pathway. This study demonstrates that gAd ameliorates osteoblastic differentiation of VSMCs possibly by blocking PI3K/AKT and Wnt/β-catenin signaling transduction. The findings provide an important foundation for gAd in treating VC in kidney diseases.

Determination of kojic acid based on the interface enhancement effects of carbon nanotube/alizarin red S modified electrode

2009 ◽  
Vol 70 (1) ◽  
pp. 20-24 ◽  
Author(s):  
Jieshu Liu ◽  
Dazhai Zhou ◽  
Xiaopeng Liu ◽  
Kangbing Wu ◽  
Chidan Wan
Keyword(s):  
Carbon Nanotube ◽  
Modified Electrode ◽  
Kojic Acid ◽  
Alizarin Red S ◽  
Alizarin Red

Cellulose Supported Thorium-Alizarin Red S Reagent for Fluoride Ion Determination

1958 ◽  
Vol 30 (9) ◽  
pp. 1485-1489 ◽  
Author(s):  
S. K. Yasuda ◽  
J. L. Lambert
Keyword(s):  
Alizarin Red S ◽  
Fluoride Ion ◽  
Alizarin Red

Spectrophotometric study of the complex of lanthanum and alizarin Red S

Talanta ◽  
1961 ◽  
Vol 8 (7) ◽  
pp. 552-556 ◽  
Author(s):  
Toshi Kawashima ◽  
Haruno Ogawa ◽  
Hiroshi Hamaguchi
Keyword(s):  
Spectrophotometric Study ◽  
Alizarin Red S ◽  
Alizarin Red

scholarly journals Automation of skeletal staining in the evaluation of teratogenicity

1969 ◽  
Vol 3 (2) ◽  
pp. 197-205 ◽  
Author(s):  
A. D. Randle
Keyword(s):  
Alizarin Red S ◽  
Alizarin Red

A number of variables affecting the quality of foetal mouse preparations stained with alizarin red S have been investigated. A seven-day 'Histokinette' procedure has been developed to macerate and stain foetal mice by a modification of Dawson's method.

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