A Scaffold-Free Allogeneic Construct From Adipose-Derived Stem Cells Regenerates an Osteochondral Defect in a Rabbit Model

Objective This study aimed to compare microfracture and application of adipose-derived stem cells (ADSCs) by local adherent technique enhanced by platelet-rich plasma (PRP) to provide a new approach for the repair of cartilage defect. Design Full-thickness cylindrical defects were created in the medial femoral condyle in 9 New Zealand White rabbits (5 months old, 4.65 ± 0.20 kg). Two groups of rabbits ( n = 3) were either treated with ADSCs (Group 1) or the microfracture technique (Group 2) following intraarticular injection of PRP 3 times in weekly intervals. Rabbits in control group ( n = 3) remained untreated. The outcome was assessed macroscopically, histologically, and immunohistochemically. Results At the end of week 12, Group 1 showed better defect filling compared with Group 2. Specimens treated with the combination of ADSCs and PRP exhibited significant differences from the other groups in all criteria of International Cartilage Repair Society macroscopic scoring system. Conclusions Intraarticular injection of autologous PRP in combination with transplantation of autologous ADSCs by local adherent technique enhances the quality of cartilage defect repair with better results in comparison with microfracture surgery in a rabbit model.

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EVALUATION OF THREE DIMENSIONAL CONSTRUCT ENGINEERED FROM POLY(LACTIC-CO-GLYCOLIC ACID)/FIBRIN HYBRID SCAFFOLD USING RABBIT BONE MARROW MESENCHYMAL STEM CELLS FOR OSTEOCHONDRAL DEFECT REPAIR

Jurnal Teknologi ◽

10.11113/jt.v77.6743 ◽

2015 ◽

Vol 77 (25) ◽

Cited By ~ 1

Author(s):

Rozlin Abdul Rahman ◽

Norhamiza Mohamad Sukri ◽

Noorhidayah Md Nazir ◽

Muhammad Aa’zamuddin Ahmad Radzi ◽

Ahmad Hafiz Zulkifly ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Glycolic Acid ◽

Osteochondral Defect ◽

Rabbit Model ◽

Three Dimensional ◽

Marker Genes ◽

Repair Capacity ◽

Marrow Mesenchymal Stem Cells

Articular cartilage has poor repair capacity due to its avascular and aneural properties and has relatively few cells. This study investigated the ability of autologous implantation approach using three dimensional (3D) constructs engineered from bone marrow mesenchymal stem cells (BMSCs) seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin as cells carrier for the repair of osteochondral defect in rabbit model. The engineered 3D constructs – PLGA/Fibrin/BMSCs and PLGA/BMSCs – were cultured for 3 weeks in vitro and implanted autologously to the osteochondral defect created in the rabbit knee. The in vivo constructs were harvested and evaluated by means of gross observation, histology assessment, gene expression study, sulphated glycosaminoglycan (sGAG) production assay and biomechanical evaluation at 6 and 12 weeks post implantation. The results showed that the osteochondral defects treated with the PLGA/Fibrin/BMSCs constructs exhibited better repairment, more cartilaginous extracellular matrix, higher sGAG production, superior compressive strength and more intense expression of chondrogenic marker genes than the PLGA/BMSCs group. This study suggested that the PLGA/Fibrin/BMSCs has the potential to treat osteochondral defect and may be presented as a viable therapeutic option for those who would be in need from the life-extending benefits of tissue replacement or repair.

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The enhanced angiogenesis effect of VEGF-silk fibroin nanospheres-BAMG scaffold composited with adipose derived stem cells in a rabbit model

RSC Advances ◽

10.1039/c7ra11610a ◽

2018 ◽

Vol 8 (27) ◽

pp. 15158-15165

Author(s):

Dongliang Zhang ◽

Nailong Cao ◽

Shukui Zhou ◽

Zhong Chen ◽

Xinru Zhang ◽

...

Keyword(s):

Stem Cells ◽

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Silk Fibroin ◽

Rabbit Model ◽

Vascular Endothelial ◽

Adipose Derived Stem Cells ◽

Acellular Matrix ◽

Endothelial Growth Factor

The adipose derived stem cells (ADSCs) was composited with vascular endothelial growth factor (VEGF)-silk fibroin (SF) nanospheres-bladder acellular matrix graft (BAMG) scaffold to repair bladder defect in rabbits.

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Vocal fold wound healing after injection of human adipose-derived stem cells in a rabbit model

Yearbook of Otolaryngology-Head and Neck Surgery ◽

10.1016/j.yoto.2012.03.029 ◽

2012 ◽

Vol 2012 ◽

pp. 59-61

Author(s):

R. Franco

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Vocal Fold ◽

Rabbit Model ◽

Adipose Derived Stem Cells

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Adipose-Derived Mesenchymal Stem Cells in the Regeneration of Vocal Folds: A Study on a Chronic Vocal Fold Scar

Stem Cells International ◽

10.1155/2016/9010279 ◽

2016 ◽

Vol 2016 ◽

pp. 1-12 ◽

Cited By ~ 18

Author(s):

Angelou Valerie ◽

Kalodimou Vassiliki ◽

Messini Irini ◽

Psychalakis Nikolaos ◽

Eleftheria Karampela ◽

...

Keyword(s):

Stem Cells ◽

Hyaluronic Acid ◽

Lamina Propria ◽

Vocal Fold ◽

Rabbit Model ◽

Vocal Folds ◽

Scar Tissue ◽

Elastic Fibers ◽

Adipose Derived Stem Cells ◽

Vocal Fold Scar

Background. The aim of the study was to assess the histological effects of autologous infusion of adipose-derived stem cells (ADSC) on a chronic vocal fold scar in a rabbit model as compared to an untreated scar as well as in injection of hyaluronic acid.Study Design. Animal experiment.Method. We used 74 New Zealand rabbits. Sixteen of them were used as control/normal group. We created a bilateral vocal fold wound in the remaining 58 rabbits. After 18 months we separated our population into three groups. The first group served as control/scarred group. The second one was injected with hyaluronic acid in the vocal folds, and the third received an autologous adipose-derived stem cell infusion in the scarred vocal folds (ADSC group). We measured the variation of thickness of the lamina propria of the vocal folds and analyzed histopathologic changes in each group after three months.Results. The thickness of the lamina propria was significantly reduced in the group that received the ADSC injection, as compared to the normal/scarred group. The collagen deposition, the hyaluronic acid, the elastin levels, and the organization of elastic fibers tend to return to normal after the injection of ADSC.Conclusions. Autologous injection of adipose-derived stem cells on a vocal fold chronic scar enhanced the healing of the vocal folds and the reduction of the scar tissue, even when compared to other treatments.

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