BRET- and fluorescence anisotropy-based assays for real-time monitoring of ligand binding to M2 muscarinic acetylcholine receptors

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research ◽

10.1016/j.bbamcr.2020.118930 ◽

2021 ◽

Vol 1868 (3) ◽

pp. 118930

Author(s):

Lukas Grätz ◽

Tõnis Laasfeld ◽

Anni Allikalt ◽

Corinna G. Gruber ◽

Andrea Pegoli ◽

...

Keyword(s):

Ligand Binding ◽

Real Time ◽

Fluorescence Anisotropy ◽

Acetylcholine Receptors ◽

Muscarinic Acetylcholine Receptors ◽

Real Time Monitoring ◽

Muscarinic Acetylcholine

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Functional role in ligand binding and receptor activation of an asparagine residue present in the sixth transmembrane domain of all muscarinic acetylcholine receptors.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)32248-2 ◽

1994 ◽

Vol 269 (29) ◽

pp. 18870-18876

Author(s):

K. Blüml ◽

E. Mutschler ◽

J. Wess

Keyword(s):

Ligand Binding ◽

Acetylcholine Receptors ◽

Functional Role ◽

Transmembrane Domain ◽

Receptor Activation ◽

Muscarinic Acetylcholine Receptors ◽

Muscarinic Acetylcholine ◽

Asparagine Residue

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Evidence for a tandem two-site model of ligand binding to muscarinic acetylcholine receptors. Vol. 275 (2000) 18836-18844

Journal of Biological Chemistry ◽

10.1016/s0021-9258(20)77092-4 ◽

2004 ◽

Vol 279 (41) ◽

pp. 43361

Author(s):

Jan Jakubík ◽

Esam E. El-Fakahany ◽

Stanislav Tuček

Keyword(s):

Ligand Binding ◽

Acetylcholine Receptors ◽

Muscarinic Acetylcholine Receptors ◽

Muscarinic Acetylcholine ◽

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Allosteric modulators of ligand binding to muscarinic acetylcholine receptors

Drug Discovery Today ◽

10.1016/s1359-6446(97)01161-6 ◽

1998 ◽

Vol 3 (5) ◽

pp. 214-222 ◽

Author(s):

Ulrike Holzgrabe ◽

Klaus Mohr

Keyword(s):

Ligand Binding ◽

Acetylcholine Receptors ◽

Muscarinic Acetylcholine Receptors ◽

Allosteric Modulators ◽

Muscarinic Acetylcholine

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Differential effects of hypoxia on ligand binding properties of nicotinic and muscarinic acetylcholine receptors on cultured bovine adrenal chromaffin cells.

The Japanese Journal of Pharmacology ◽

10.1016/s0021-5198(19)39212-1 ◽

1991 ◽

Vol 55 ◽

pp. 275

Author(s):

Ken Lee ◽

Kunio Koshimura ◽

Tetsuya Ohue ◽

Soichi Miwa

Keyword(s):

Ligand Binding ◽

Acetylcholine Receptors ◽

Chromaffin Cells ◽

Muscarinic Acetylcholine Receptors ◽

Adrenal Chromaffin Cells ◽

Binding Properties ◽

Differential Effects ◽

Muscarinic Acetylcholine ◽

Adrenal Chromaffin

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Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.

The EMBO Journal ◽

10.1002/j.1460-2075.1987.tb02733.x ◽

1987 ◽

Vol 6 (13) ◽

pp. 3923-3929 ◽

Author(s):

E. G. Peralta ◽

A. Ashkenazi ◽

J. W. Winslow ◽

D. H. Smith ◽

J. Ramachandran ◽

...

Keyword(s):

Ligand Binding ◽

Acetylcholine Receptors ◽

Muscarinic Acetylcholine Receptors ◽

Binding Properties ◽

Specific Expression ◽

Tissue Specific ◽

Tissue Specific Expression ◽

Muscarinic Acetylcholine ◽

Primary Structures

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Effects of guanine nucleotides on ligand binding to muscarinic acetylcholine receptors in Torpedo electric organ

Biochemical Society Transactions ◽

10.1042/bst0090412 ◽

1981 ◽

Vol 9 (5) ◽

pp. 412-413 ◽

Author(s):

M. J. DOWDALL ◽

P. R. GOLDS ◽

P. G. STRANGE

Keyword(s):

Ligand Binding ◽

Acetylcholine Receptors ◽

Electric Organ ◽

Guanine Nucleotides ◽

Muscarinic Acetylcholine Receptors ◽

Muscarinic Acetylcholine ◽

Torpedo Electric Organ

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Effects of short-term toluene exposure on ligand binding to muscarinic acetylcholine receptors in the rat frontal cortex and hippocampus

Neurotoxicology and Teratology ◽

10.1016/s0892-0362(00)00081-7 ◽

2000 ◽

Vol 22 (4) ◽

pp. 603-606 ◽

Author(s):

Hirofumi Tsuga ◽

Takeshi Honma

Keyword(s):

Ligand Binding ◽

Frontal Cortex ◽

Acetylcholine Receptors ◽

Muscarinic Acetylcholine Receptors ◽

Muscarinic Acetylcholine ◽

Toluene Exposure ◽

Rat Frontal Cortex

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Evidence for a Tandem Two-site Model of Ligand Binding to Muscarinic Acetylcholine Receptors

Journal of Biological Chemistry ◽

10.1074/jbc.m000112200 ◽

2000 ◽

Vol 275 (25) ◽

pp. 18836-18844 ◽

Author(s):

Jan Jakubı́k ◽

Esam E. El-Fakahany ◽

Stanislav Tuc̆ek

Keyword(s):

Ligand Binding ◽

Acetylcholine Receptors ◽

Muscarinic Acetylcholine Receptors ◽

Muscarinic Acetylcholine ◽

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Differential Effects of Hypoxia on Ligand Binding Properties of Nicotinic and Muscarinic Acetylcholine Receptors on Cultured Bovine Adrenal Chromaffin Cells

Journal of Neurochemistry ◽

10.1046/j.1471-4159.1995.64020874.x ◽

2002 ◽

Vol 64 (2) ◽

pp. 874-882 ◽

Author(s):

Ken Lee ◽

Akihiro Ito ◽

Kunio Koshimura ◽

Tetsuya Ohue ◽

Yasutaka Takagi ◽

...

Keyword(s):

Ligand Binding ◽

Acetylcholine Receptors ◽

Chromaffin Cells ◽

Muscarinic Acetylcholine Receptors ◽

Adrenal Chromaffin Cells ◽

Binding Properties ◽

Differential Effects ◽

Muscarinic Acetylcholine ◽

Adrenal Chromaffin

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Transition and heavy metal inhibition of ligand binding to muscarinic acetylcholine receptors from rat brain

Toxicology and Applied Pharmacology ◽

10.1016/0041-008x(79)90432-0 ◽

1979 ◽

Vol 48 (3) ◽

pp. 489-496 ◽

Author(s):

Robert S. Aronstam ◽

Mohyee E. Eldefrawi

Keyword(s):

Heavy Metal ◽

Ligand Binding ◽

Rat Brain ◽

Acetylcholine Receptors ◽

Muscarinic Acetylcholine Receptors ◽

Muscarinic Acetylcholine

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