Differential Effects of Hypoxia on Ligand Binding Properties of Nicotinic and Muscarinic Acetylcholine Receptors on Cultured Bovine Adrenal Chromaffin Cells

2002 ◽  
Vol 64 (2) ◽  
pp. 874-882 ◽  
Author(s):  
Ken Lee ◽  
Akihiro Ito ◽  
Kunio Koshimura ◽  
Tetsuya Ohue ◽  
Yasutaka Takagi ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Acetylcholine Receptors ◽  
Chromaffin Cells ◽  
Muscarinic Acetylcholine Receptors ◽  
Adrenal Chromaffin Cells ◽  
Binding Properties ◽  
Differential Effects ◽  
Muscarinic Acetylcholine ◽  
Adrenal Chromaffin

scholarly journals Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.

1987 ◽  
Vol 6 (13) ◽  
pp. 3923-3929 ◽  
Author(s):  
E. G. Peralta ◽  
A. Ashkenazi ◽  
J. W. Winslow ◽  
D. H. Smith ◽  
J. Ramachandran ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Acetylcholine Receptors ◽  
Muscarinic Acetylcholine Receptors ◽  
Binding Properties ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Muscarinic Acetylcholine ◽  
Primary Structures

scholarly journals Muscarinic inhibition of nicotinic transmission in rat sympathetic neurons and adrenal chromaffin cells

2015 ◽  
Vol 370 (1672) ◽  
pp. 20140188 ◽  
Author(s):  
Lin-Ling He ◽  
Quan-Feng Zhang ◽  
Lie-Cheng Wang ◽  
Jing-Xia Dai ◽  
Chang-He Wang ◽  
...  
Keyword(s):  
Protein Kinase ◽  
G Protein ◽  
Acetylcholine Receptors ◽  
Chromaffin Cells ◽  
Sympathetic Neurons ◽  
Second Messenger ◽  
Muscarinic Acetylcholine Receptors ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin ◽  
Ganglion Neurons

Little is known about the interactions between nicotinic and muscarinic acetylcholine receptors (nAChRs and mAChRs). Here we report that methacholine (MCh), a selective agonist of mAChRs, inhibited up to 80% of nicotine-induced nAChR currents in sympathetic superior cervical ganglion neurons and adrenal chromaffin cells. The muscarine-induced inhibition (MiI) substantially reduced ACh-induced membrane currents through nAChRs and quantal neurotransmitter release. The MiI was time- and temperature-dependent. The slow recovery of nAChR current after washout of MCh, as well as the high value of Q10 (3.2), suggested, instead of a direct open-channel blockade, an intracellular metabotropic process. The effects of GTP-γ-S, GDP-β-S and pertussis toxin suggested that MiI was mediated by G-protein signalling. Inhibitors of protein kinase C (bisindolymaleimide–Bis), protein kinase A (H89) and PIP2 depletion attenuated the MiI, indicating that a second messenger pathway is involved in this process. Taken together, these data suggest that mAChRs negatively modulated nAChRs via a G-protein-mediated second messenger pathway. The time dependence suggests that MiI may provide a novel mechanism for post-synaptic adaptation in all cells/neurons and synapses expressing both types of AChRs.

scholarly journals BRET- and fluorescence anisotropy-based assays for real-time monitoring of ligand binding to M2 muscarinic acetylcholine receptors

2021 ◽  
Vol 1868 (3) ◽  
pp. 118930
Author(s):  
Lukas Grätz ◽  
Tõnis Laasfeld ◽  
Anni Allikalt ◽  
Corinna G. Gruber ◽  
Andrea Pegoli ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Real Time ◽  
Fluorescence Anisotropy ◽  
Acetylcholine Receptors ◽  
Muscarinic Acetylcholine Receptors ◽  
Real Time Monitoring ◽  
Muscarinic Acetylcholine

scholarly journals Expression of α3β2β4 nicotinic acetylcholine receptors by rat adrenal chromaffin cells determined using novel conopeptide antagonists

2020 ◽  
Vol 154 (2) ◽  
pp. 158-176 ◽  
Author(s):  
Arik J. Hone ◽  
Lola Rueda‐Ruzafa ◽  
Thomas J. Gordon ◽  
Joanna Gajewiak ◽  
Sean Christensen ◽  
...  
Keyword(s):  
Nicotinic Acetylcholine Receptors ◽  
Acetylcholine Receptors ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Nicotinic Acetylcholine ◽  
Adrenal Chromaffin

scholarly journals Short- and long-term differential effects of neuroprotective drug NS-7 on voltage-dependent sodium channels in adrenal chromaffin cells

2000 ◽  
Vol 131 (4) ◽  
pp. 779-787 ◽  
Author(s):  
Hiroki Yokoo ◽  
Seiji Shiraishi ◽  
Hideyuki Kobayashi ◽  
Toshihiko Yanagita ◽  
Shin-ichi Minami ◽  
...  
Keyword(s):  
Sodium Channels ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Differential Effects ◽  
Voltage Dependent ◽  
Short And Long Term ◽  
Adrenal Chromaffin
Sign in / Sign up

Export Citation Format

Share Document