scholarly journals The temperature dependence of the BK channel activity – kinetics, thermodynamics, and long-range correlations

2017 ◽  
Vol 1859 (10) ◽  
pp. 1805-1814 ◽  
Author(s):  
Agata Wawrzkiewicz-Jałowiecka ◽  
Beata Dworakowska ◽  
Zbigniew J. Grzywna
Keyword(s):  
Temperature Dependence ◽  
Long Range ◽  
Bk Channel ◽  
Channel Activity ◽  
Long Range Correlations

scholarly journals Differences in Gating Dynamics of BK Channels in Cellular and Mitochondrial Membranes from Human Glioblastoma Cells Unraveled by Short- and Long-Range Correlations Analysis

Cells ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 2305 ◽  
Author(s):  
Agata Wawrzkiewicz-Jałowiecka ◽  
Paulina Trybek ◽  
Przemysław Borys ◽  
Beata Dworakowska ◽  
Łukasz Machura ◽  
...  
Keyword(s):  
Long Range ◽  
Single Channel ◽  
Cellular Membrane ◽  
Bk Channels ◽  
Bk Channel ◽  
Fluctuation Analysis ◽  
Channel Activity ◽  
Human Glioblastoma ◽  
Long Range Correlations ◽  
Single Channel Activity

The large-conductance voltage- and Ca2+-activated K+ channels (BK) are encoded in humans by the Kcnma1 gene. Nevertheless, BK channel isoforms in different locations can exhibit functional heterogeneity mainly due to the alternative splicing during the Kcnma1 gene transcription. Here, we would like to examine the existence of dynamic diversity of BK channels from the inner mitochondrial and cellular membrane from human glioblastoma (U-87 MG). Not only the standard characteristics of the spontaneous switching between the functional states of the channel is discussed, but we put a special emphasis on the presence and strength of correlations within the signal describing the single-channel activity. The considered short- and long-range memory effects are here analyzed as they can be interpreted in terms of the complexity of the switching mechanism between stable conformational states of the channel. We calculate the dependencies of mean dwell-times of (conducting/non-conducting) states on the duration of the previous state, Hurst exponents by the rescaled range R/S method and detrended fluctuation analysis (DFA), and use the multifractal extension of the DFA (MFDFA) for the series describing single-channel activity. The obtained results unraveled statistically significant diversity in gating machinery between the mitochondrial and cellular BK channels.

scholarly journals Studies of charm and beauty hadron long-range correlations in pp and pPb collisions at LHC energies

2021 ◽  
Vol 813 ◽  
pp. 136036
Author(s):  
A.M. Sirunyan ◽  
A. Tumasyan ◽  
W. Adam ◽  
F. Ambrogi ◽  
T. Bergauer ◽  
...  
Keyword(s):  
Long Range ◽  
Long Range Correlations

scholarly journals Long-range correlations in pinned athermal networks

2021 ◽  
Vol 104 (1) ◽  
Author(s):  
Debankur Das ◽  
Pappu Acharya ◽  
Kabir Ramola
Keyword(s):  
Long Range ◽  
Long Range Correlations

scholarly journals Large-conductance voltage- and Ca2+-activated K+ channel regulation by protein kinase C in guinea pig urinary bladder smooth muscle

2014 ◽  
Vol 306 (5) ◽  
pp. C460-C470 ◽  
Author(s):  
Kiril L. Hristov ◽  
Amy C. Smith ◽  
Shankar P. Parajuli ◽  
John Malysz ◽  
Georgi V. Petkov
Keyword(s):  
Smooth Muscle ◽  
Membrane Potential ◽  
Guinea Pig ◽  
Bk Channels ◽  
Bk Channel ◽  
K Channel ◽  
Channel Activity ◽  
Open Probability ◽  
Channel Regulation ◽  
Pkc Activation

Large-conductance voltage- and Ca2+-activated K+ (BK) channels are critical regulators of detrusor smooth muscle (DSM) excitability and contractility. PKC modulates the contraction of DSM and BK channel activity in non-DSM cells; however, the cellular mechanism regulating the PKC-BK channel interaction in DSM remains unknown. We provide a novel mechanistic insight into BK channel regulation by PKC in DSM. We used patch-clamp electrophysiology, live-cell Ca2+ imaging, and functional studies of DSM contractility to elucidate BK channel regulation by PKC at cellular and tissue levels. Voltage-clamp experiments showed that pharmacological activation of PKC with PMA inhibited the spontaneous transient BK currents in native freshly isolated guinea pig DSM cells. Current-clamp recordings revealed that PMA significantly depolarized DSM membrane potential and inhibited the spontaneous transient hyperpolarizations in DSM cells. The PMA inhibitory effects on DSM membrane potential were completely abolished by the selective BK channel inhibitor paxilline. Activation of PKC with PMA did not affect the amplitude of the voltage-step-induced whole cell steady-state BK current or the single BK channel open probability (recorded in cell-attached mode) upon inhibition of all major Ca2+ sources for BK channel activation with thapsigargin, ryanodine, and nifedipine. PKC activation with PMA elevated intracellular Ca2+ levels in DSM cells and increased spontaneous phasic and nerve-evoked contractions of DSM isolated strips. Our results support the concept that PKC activation leads to a reduction of BK channel activity in DSM via a Ca2+-dependent mechanism, thus increasing DSM contractility.

LONG-RANGE CORRELATIONS IN THE PHASE-SHIFTS OF NUMERICAL SIMULATIONS OF BIOCHEMICAL OSCILLATIONS AND IN EXPERIMENTAL CARDIAC RHYTHMS

1999 ◽  
Vol 07 (02) ◽  
pp. 113-130 ◽  
Author(s):  
I. M. DE LA FUENTE ◽  
L. MARTINEZ ◽  
J. M. AGUIRREGABIRIA ◽  
J. VEGUILLAS ◽  
M. IRIARTE
Keyword(s):  
Phase Shift ◽  
Periodic Solutions ◽  
Long Range ◽  
Phase Shifts ◽  
Long Range Correlations ◽  
Rescaled Range ◽  
Biochemical Oscillations ◽  
Phase Shift Data ◽  
Relationship Of ◽  
The Relationship

In biochemical dynamical systems during each transition between periodical behaviors, all metabolic intermediaries of the system oscillate with the same frequency but with different phase-shifts. We have studied the behavior of phase-shift records obtained from random transitions between periodic solutions of a biochemical dynamical system. The phase-shift data were analyzed by means of Hurst's rescaled range method (introduced by Mandelbrot and Wallis). The results show the existence of persistent behavior: each value of the phase-shift depends not only on the recent transitions, but also on previous ones. In this paper, the different kind of periodic solutions were determined by different small values of the control parameter. It was assessed the significance of this results through extensive Monte Carlo simulations as well as quantifying the long-range correlations. We have also applied this type of analysis on cardiac rhythms, showing a clear persistent behavior. The relationship of the results with the cellular persistence phenomena conditioned by the past, widely evidenced in experimental observations, is discussed.

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