scholarly journals Kinetic analysis of PCNA clamp binding and release in the clamp loading reaction catalyzed by Saccharomyces cerevisiae replication factor C

2015 ◽  
Vol 1854 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Melissa R. Marzahn ◽  
Jaclyn N. Hayner ◽  
Jennifer A. Meyer ◽  
Linda B. Bloom
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Kinetic Analysis ◽  
Replication Factor C ◽  
Replication Factor ◽  
Clamp Loading ◽  
Factor C

scholarly journals The Large Subunit of Replication Factor C (Rfclp/Cdc44p) Is Required for DNA Replication and DNA Repair in Saccharomyces cerevisiae

Genetics ◽  
1996 ◽  
Vol 142 (1) ◽  
pp. 65-78 ◽  
Author(s):  
Michael A McAlear ◽  
K Michelle Tuffo ◽  
Connie Holm
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Repair ◽  
Dna Replication ◽  
Uv Radiation ◽  
Large Subunit ◽  
Restrictive Temperature ◽  
Replication Factor C ◽  
Replication Factor ◽  
Factor C

We used genetic and biochemical techniques to characterize the phenotypes associated with mutations affecting the large subunit of replication factor C (Cdc44p or Rfc1p) in Saccharomyces cerevisiae. We demonstrate that Cdc44p is required for both DNA replication and DNA repair in vivo. Cold-sensitive cdc44 mutants experience a delay in traversing S phase at the restrictive temperature following alpha factor arrest; although mutant cells eventually accumulate with a G2/M DNA content, they undergo a cell cycle arrest and initiate neither mitosis nor a new round of DNA synthesis. cdc44 mutants also exhibit an elevated level of spontaneous mutation, and they are sensitive both to the DNA damaging agent methylmethane sulfonate and to exposure to UV radiation. After exposure to UV radiation, cdc44 mutants at the restrictive temperature contain higher levels of single-stranded DNA breaks than do wild-type cells. This observation is consistent with the hypothesis that Cdc44p is involved in repairing gaps in the DNA after the excision of damaged bases. Thus, Cdc44p plays an important role in both DNA replication and DNA repair in vivo.

scholarly journals Physical Links Between the Nuclear Envelope Protein Mps3, Three Alternate Replication Factor C Complexes, and a Variant Histone in Saccharomyces cerevisiae

2012 ◽  
Vol 31 (6) ◽  
pp. 917-924 ◽  
Author(s):  
Jared Haas ◽  
Amanda Lemoncelli ◽  
Christina Morozov ◽  
Karl Franke ◽  
John Dominder ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nuclear Envelope ◽  
Envelope Protein ◽  
Replication Factor C ◽  
Replication Factor ◽  
Factor C
Sign in / Sign up

Export Citation Format

Share Document