Identification and characterization of a transcriptional regulator, SucR, that influences sucCD transcription in Corynebacterium glutamicum

2010 ◽  
Vol 401 (2) ◽  
pp. 300-305 ◽  
Author(s):  
Hyun-Young Cho ◽  
Seong Gyu Lee ◽  
Jeong Eun Hyeon ◽  
Sung Ok Han
Keyword(s):  
Corynebacterium Glutamicum ◽  
Transcriptional Regulator ◽  
Identification And Characterization

scholarly journals Identification and Characterization of OscR, a Transcriptional Regulator Involved in Osmolarity Adaptation in Vibrio cholerae

2009 ◽  
Vol 191 (13) ◽  
pp. 4082-4096 ◽  
Author(s):  
Nicholas J. Shikuma ◽  
Fitnat H. Yildiz
Keyword(s):  
Vibrio Cholerae ◽  
Biofilm Formation ◽  
Transcriptional Regulator ◽  
Dependent Manner ◽  
Genome Wide ◽  
Low Salt ◽  
Adaptation Response ◽  
Matrix Production ◽  
Identification And Characterization

ABSTRACT Vibrio cholerae is a facultative human pathogen. In its aquatic habitat and as it passes through the digestive tract, V. cholerae must cope with fluctuations in salinity. We analyzed the genome-wide transcriptional profile of V. cholerae grown at different NaCl concentrations and determined that the expression of compatible solute biosynthesis and transporter genes, virulence genes, and genes involved in adhesion and biofilm formation is differentially regulated. We determined that salinity modulates biofilm formation, and this response was mediated through the transcriptional regulators VpsR and VpsT. Additionally, a transcriptional regulator controlling an osmolarity adaptation response was identified. This regulator, OscR (osmolarity controlled regulator), was found to modulate the transcription of genes involved in biofilm matrix production and motility in a salinity-dependent manner. oscR mutants were less motile and exhibited enhanced biofilm formation only under low-salt conditions.

scholarly journals Identification and Characterization of a Transcriptional Regulator for thelckProximal Promoter

2001 ◽  
Vol 276 (21) ◽  
pp. 18082-18089 ◽  
Author(s):  
Atsuko Yamada ◽  
Satoshi Takaki ◽  
Fumitaka Hayashi ◽  
Katia Georgopoulos ◽  
Roger M. Perlmutter ◽  
...  
Keyword(s):  
Transcriptional Regulator ◽  
Identification And Characterization

scholarly journals Identification and Characterization of EctR1, a New Transcriptional Regulator of the Ectoine Biosynthesis Genes in the Halotolerant Methanotroph Methylomicrobium alcaliphilum 20Z

2009 ◽  
Vol 192 (2) ◽  
pp. 410-417 ◽  
Author(s):  
Ildar I. Mustakhimov ◽  
Alexander S. Reshetnikov ◽  
Anatoly S. Glukhov ◽  
Valentina N. Khmelenina ◽  
Marina G. Kalyuzhnaya ◽  
...  
Keyword(s):  
Binding Site ◽  
Transcriptional Regulator ◽  
Negative Regulator ◽  
Reading Frame ◽  
Genes Encoding ◽  
Methylomicrobium Alcaliphilum ◽  
Translational Start ◽  
Identification And Characterization ◽  
Ectoine Biosynthesis

ABSTRACTGenes encoding key enzymes of the ectoine biosynthesis pathway in the halotolerant obligate methanotrophMethylomicrobium alcaliphilum20Z have been shown to be organized into anectABC-askoperon. Transcription of theectoperon is initiated from two promoters,ectAp1andectAp2(ectAp1p2), similar to the σ70-dependent promoters ofEscherichia coli. Upstream of the gene cluster, an open reading frame (ectR1) encoding a MarR-like transcriptional regulator was identified. Investigation of the influence of EctR1 on the activity of theectAp1p2promoters in wild-typeM. alcaliphilum20Z andectR1mutant strains suggested that EctR1 is a negative regulator of theectABC-askoperon. Purified recombinant EctR1-His6specifically binds as a homodimer to the putative −10 motif of theectAp1promoter. The EctR1 binding site contains a pseudopalindromic sequence (TATTTAGT-GT-ACTATATA) composed of 8-bp half-sites separated by 2 bp. Transcription of theectR1gene is initiated from a single σ70-like promoter. The location of the EctR1 binding site between the transcriptional and translational start sites of theectR1gene suggests that EctR1 may regulate its own expression. The data presented suggest that inMethylomicrobium alcaliphilum20Z, EctR1-mediated control of the transcription of theectgenes is not the single mechanism for the regulation of ectoine biosynthesis.

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