Estimating the affinity of protein–ligand complex from changes to the charge-state distribution of a protein in electrospray ionization mass spectrometry

Elizabeth A. Blackburn; John K.F. Maclean; Bradley S. Sherborne; Malcolm D. Walkinshaw

doi:10.1016/j.bbrc.2010.11.003

Estimating the affinity of protein–ligand complex from changes to the charge-state distribution of a protein in electrospray ionization mass spectrometry

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2010.11.003 ◽

2010 ◽

Vol 403 (2) ◽

pp. 190-193 ◽

Cited By ~ 6

Author(s):

Elizabeth A. Blackburn ◽

John K.F. Maclean ◽

Bradley S. Sherborne ◽

Malcolm D. Walkinshaw

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Charge State Distribution ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

State Distribution ◽

Ligand Complex

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Modifying the charge state distribution of proteins in electrospray ionization mass spectrometry by chemical derivatization

Journal of the American Society for Mass Spectrometry ◽

10.1016/j.jasms.2009.04.017 ◽

2009 ◽

Vol 20 (9) ◽

pp. 1617-1625 ◽

Cited By ~ 46

Author(s):

Casey J. Krusemark ◽

Brian L. Frey ◽

Peter J. Belshaw ◽

Lloyd M. Smith

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Charge State Distribution ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Chemical Derivatization ◽

State Distribution

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Charge State Distribution and Hydrogen/Deuterium Exchange of α-Lactalbumin and β-Lactoglobulin Preparations by Electrospray Ionization Mass Spectrometry

Journal of Agricultural and Food Chemistry ◽

10.1021/jf020816e ◽

2003 ◽

Vol 51 (7) ◽

pp. 2049-2057 ◽

Cited By ~ 10

Author(s):

Husam Alomirah ◽

Inteaz Alli ◽

Yasuo Konishi

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Charge State Distribution ◽

Ionization Mass ◽

Hydrogen Deuterium Exchange ◽

State Distribution ◽

Hydrogen Deuterium ◽

Β Lactoglobulin

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Sample Flow Rate Scan in Electrospray Ionization Mass Spectrometry Reveals Alterations in Protein Charge State Distribution

Analytical Chemistry ◽

10.1021/acs.analchem.0c01945 ◽

2020 ◽

Vol 92 (19) ◽

pp. 13042-13049

Author(s):

Gurpur Rakesh D. Prabhu ◽

Vinoth Kumar Ponnusamy ◽

Henryk A. Witek ◽

Pawel L. Urban

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Charge State ◽

Flow Rate ◽

Electrospray Ionization Mass Spectrometry ◽

Charge State Distribution ◽

Ionization Mass ◽

State Distribution ◽

Protein Charge ◽

Sample Flow Rate

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Analysis of mixtures of closely related forms of bovine trypsin by electrospray ionization mass spectrometry: Use of charge state distributions to resolve ions of the different forms

Biochemical and Biophysical Research Communications ◽

10.1016/s0006-291x(05)80874-5 ◽

1990 ◽

Vol 173 (3) ◽

pp. 927-931 ◽

Cited By ~ 13

Author(s):

S.K. Chowdhury ◽

B.T. Chait

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Bovine Trypsin ◽

Charge State Distributions

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Charge-state reduction with improved signal intensity of oligonucleotides in electrospray ionization mass spectrometry

Journal of the American Society for Mass Spectrometry ◽

10.1016/s1044-0305(96)80516-2 ◽

1996 ◽

Vol 7 (8) ◽

pp. 697-706

Author(s):

D MUDDIMAN ◽

X CHENG ◽

H UDSETH ◽

R SMITH

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Signal Intensity ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

State Reduction

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Protein Charge-State Distributions in Electrospray-Ionization Mass Spectrometry Do Not Appear To Be Limited by the Surface Tension of the Solvent

Journal of the American Chemical Society ◽

10.1021/ja037000u ◽

2003 ◽

Vol 125 (44) ◽

pp. 13352-13353 ◽

Cited By ~ 50

Author(s):

Maria Šamalikova ◽

Rita Grandori

Keyword(s):

Mass Spectrometry ◽

Surface Tension ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Protein Charge ◽

Charge State Distributions

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ESIprot: a universal tool for charge state determination and molecular weight calculation of proteins from electrospray ionization mass spectrometry data

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.4384 ◽

2010 ◽

Vol 24 (3) ◽

pp. 285-294 ◽

Cited By ~ 38

Author(s):

Robert Winkler

Keyword(s):

Mass Spectrometry ◽

Molecular Weight ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Mass Spectrometry Data ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

State Determination ◽

Weight Calculation

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Response surface study on protein charge–state distributions in electrospray ionization mass spectrometry related to multiple ion equilibria in solution

Chemometrics and Intelligent Laboratory Systems ◽

10.1016/j.chemolab.2004.01.024 ◽

2004 ◽

Vol 72 (2) ◽

pp. 245-252 ◽

Cited By ~ 5

Author(s):

Matthias Otto ◽

Franziska Knabe ◽

Ute Claußnitzer

Keyword(s):

Mass Spectrometry ◽

Response Surface ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Protein Charge ◽

Surface Study ◽

Charge State Distributions

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Origin of the conformation dependence of protein charge-state distributions in electrospray ionization mass spectrometry

Journal of Mass Spectrometry ◽

10.1002/jms.390 ◽

2003 ◽

Vol 38 (1) ◽

pp. 11-15 ◽

Cited By ~ 95

Author(s):

Rita Grandori

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Protein Charge ◽

Charge State Distributions

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Electrospray Ionization Mass Spectrometry of Highly Heterogeneous Protein Systems: Protein Ion Charge State Assignment via Incomplete Charge Reduction

Analytical Chemistry ◽

10.1021/ac101848z ◽

2010 ◽

Vol 82 (18) ◽

pp. 7523-7526 ◽

Cited By ~ 47

Author(s):

Rinat R. Abzalimov ◽

Igor A. Kaltashov

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Charge State ◽

Electrospray Ionization Mass Spectrometry ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Charge Reduction ◽

State Assignment

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