The WNT inhibitor APCDD1 sustains the expression of β-Catenin during the osteogenic differentiation of human dental follicle cells

2015 ◽  
Vol 457 (3) ◽  
pp. 314-317 ◽  
Author(s):  
S. Viale-Bouroncle ◽  
C. Klingelhöffer ◽  
T. Ettl ◽  
C. Morsczeck
Keyword(s):  
Osteogenic Differentiation ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Wnt Inhibitor

scholarly journals Microarray Analysis of Human Dental Follicle Cells in Osteogenic Differentiation

2009 ◽  
Vol 18 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Haruna Aonuma ◽  
Naomi Ogura ◽  
Yoshikazu Kamino ◽  
Ko Ito ◽  
Toshirou Kondoh
Keyword(s):  
Osteogenic Differentiation ◽  
Microarray Analysis ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

scholarly journals Butyrate stimulates the early process of the osteogenic differentiation but inhibits the biomineralization in dental follicle cells (DFCs)

Odontology ◽  
2013 ◽  
Vol 102 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Johannes Drees ◽  
Oliver Felthaus ◽  
Martin Gosau ◽  
Christian Morsczeck
Keyword(s):  
Osteogenic Differentiation ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

scholarly journals AFF4 regulates osteogenic differentiation of human dental follicle cells

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Qingyue Xiao ◽  
Yuning Zhang ◽  
Xingying Qi ◽  
Yaqian Chen ◽  
Rui Sheng ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

Hertwig's epithelial root sheath cells regulate osteogenic differentiation of dental follicle cells through the Wnt pathway

Bone ◽  
2014 ◽  
Vol 63 ◽  
pp. 158-165 ◽  
Author(s):  
Yaling Yang ◽  
Yaneng Ge ◽  
Guoqing Chen ◽  
Zhiling Yan ◽  
Mei Yu ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Wnt Pathway ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Hertwig’S Epithelial Root Sheath ◽  
Root Sheath ◽  
Sheath Cells

scholarly journals Classical isoforms of protein kinase C (PKC) and Akt regulate the osteogenic differentiation of human dental follicle cells via both β-catenin and NF-κB

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Oliver Pieles ◽  
Torsten E. Reichert ◽  
Christian Morsczeck
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Osteogenic Differentiation ◽  
Precursor Cells ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Alizarin Red ◽  
Dental Follicle Cells ◽  
Parathyroid Hormone Related Protein ◽  
Kinase C

Abstract Background Human dental follicle cells (DFCs) are the precursor cells of the periodontium with a high potential for regenerative therapies of (alveolar) bone. However, the molecular mechanisms of osteogenic differentiation are inadequately understood. Classical isoforms of protein kinase C (PKC) are reported to inhibit osteogenesis of stem/precursor cells. This study evaluated the role of classical PKCs and potential downstream targets on the osteogenic differentiation of DFCs. Methods DFCs were osteogenic differentiated with dexamethasone or bone morphogenetic protein 2 (BMP2). Expression of PKC and potential upstream/downstream regulators was manipulated using activators, inhibitors, and small interfering ribonucleic acid (siRNA). Expression of proteins was examined by Western blot analysis, while the activation levels of enzymes and transcription factors were examined by their phosphorylation states or by specific activation assays. Expression levels of osteogenic markers were examined by RT-qPCR (reverse transcription-quantitative polymerase chain reaction) analysis. Activity of alkaline phosphatase (ALP) and accumulation of calcium nodules by Alizarin Red staining were measured as indicators of mineralization. Results Classical PKCs like PKCα inhibit the osteogenic differentiation of DFCs, but do not interfere with the induction of differentiation. Inhibition of classical PKCs by Gö6976 enhanced activity of Akt after osteogenic induction. Akt was also regulated during differentiation and especially disturbed BMP2-induced mineralization. The PKC/Akt axis was further shown to regulate the canonical Wnt signaling pathway and eventually nuclear expression of active β-catenin during dexamethasone-induced osteogenesis. Moreover, the nuclear factor “kappa-light-chain-enhancer” of activated B cells (NF-κB) pathway is regulated during osteogenic differentiation of DFCs and via the PKC/Akt axis and disturbs the mineralization. Upstream, parathyroid hormone-related protein (PTHrP) sustained the activity of PKC, while Wnt5a inhibited it. Conclusions Our results demonstrate that classical PKCs like PKCα and Akt regulate the osteogenic differentiation of DFCs partly via both β-catenin and NF-κB.

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