The AKT signaling pathway sustains the osteogenic differentiation in human dental follicle cells

2015 ◽  
Vol 406 (1-2) ◽  
pp. 199-204 ◽  
Author(s):  
S. Viale-Bouroncle ◽  
C. Klingelhöffer ◽  
T. Ettl ◽  
C. Morsczeck
Keyword(s):  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Akt Signaling Pathway ◽  
Dental Follicle Cells

scholarly journals Microarray Analysis of Human Dental Follicle Cells in Osteogenic Differentiation

2009 ◽  
Vol 18 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Haruna Aonuma ◽  
Naomi Ogura ◽  
Yoshikazu Kamino ◽  
Ko Ito ◽  
Toshirou Kondoh
Keyword(s):  
Osteogenic Differentiation ◽  
Microarray Analysis ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

scholarly journals Astragaloside positively regulated osteogenic differentiation of pre-osteoblast MC3T3-E1 through PI3K/Akt signaling pathway

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Wei Bing Jing ◽  
Hongjuan Ji ◽  
Rui Jiang ◽  
Jinlong Wang
Keyword(s):  
Western Blot ◽  
Osteogenic Differentiation ◽  
Real Time ◽  
Signaling Pathway ◽  
Real Time Pcr ◽  
Akt Signaling ◽  
Calcium Deposition ◽  
Control Group ◽  
Akt Signaling Pathway ◽  
Western Blot Assay

Abstract Background Osteoporosis is a widespread chronic disease characterized by low bone density. There is currently no gold standard treatment for osteoporosis. The aim of this study was to explore the role and mechanism of Astragaloside on osteogenic differentiation of MC3T3-E1 cells. Methods MC3T3-E1 cells were divided into control and different dose of Astragaloside (10, 20, 40, 50, and 60 μg/ml). Then, ALP and ARS staining were performed to identify the effects of Astragaloside for early and late osteogenic capacity of MC3T3-E1 cells, respectively. Real-time PCR and western blot were performed to assess the ALP, OCN, and OSX expression. PI3K/Akt signaling pathway molecules were then assessed by Western blot. Finally, PI3K inhibitor, LY294002, was implemented to assess the mechanism of Astragaloside in promoting osteogenic differentiation of MC3T3-E1 cells. Results Astragaloside significantly increased the cell viability than the control group. Moreover, Astragaloside enhanced the ALP activity and calcium deposition than the control groups. Compared with the control group, Astragaloside increased the ALP, OCN, and OSX expression in a dose-response manner. Western blot assay further confirmed the real-time PCR results. Astragaloside could significantly increase the p-PI3K and p-Akt expression than the control group. LY294002 partially reversed the promotion effects of Astragaloside on osteogenic differentiation of MC3T3-E1 cells. LY294002 partially reversed the promotion effects of Astragaloside on ALP, OCN, and OSX of MC3T3-E1 cells. Conclusion The present study suggested that Astragaloside promoted osteogenic differentiation of MC3T3-E1 cells through regulating PI3K/Akt signaling pathway.

scholarly journals Butyrate stimulates the early process of the osteogenic differentiation but inhibits the biomineralization in dental follicle cells (DFCs)

Odontology ◽  
2013 ◽  
Vol 102 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Johannes Drees ◽  
Oliver Felthaus ◽  
Martin Gosau ◽  
Christian Morsczeck
Keyword(s):  
Osteogenic Differentiation ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

scholarly journals AFF4 regulates osteogenic differentiation of human dental follicle cells

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Qingyue Xiao ◽  
Yuning Zhang ◽  
Xingying Qi ◽  
Yaqian Chen ◽  
Rui Sheng ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells
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