Neural differentiation of human embryonic stem cells induced by the transgene-mediated overexpression of single transcription factors

2017 ◽  
Vol 490 (2) ◽  
pp. 296-301 ◽  
Author(s):  
Misako Matsushita ◽  
Yuhki Nakatake ◽  
Itaru Arai ◽  
Keiji Ibata ◽  
Kazuhisa Kohda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem

scholarly journals PVDF Nanofiber Scaffold Coated with a Vitronectin Peptide Facilitates the Neural Differentiation of Human Embryonic Stem Cells

2020 ◽  
Vol 24 (2) ◽  
pp. 135-147
Author(s):  
Byeong-Min Jeon ◽  
Gyu-Bum Yeon ◽  
Hui-Gwan Goo ◽  
Kyung Eun Lee ◽  
Dae-Sung Kim
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Nanofiber Scaffold

304 THE DEVELOPMENTAL TOXICITY EVALUATION OF 5-FLUOROURACIL AND INDOMETHACIN IN HUMAN EMBRYONIC STEM CELLS

2013 ◽  
Vol 25 (1) ◽  
pp. 299
Author(s):  
E. M. Jung ◽  
E. B. Jeung
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Developmental Process ◽  
Developmental Toxicity ◽  
Embryonic Stem ◽  
Surface Markers ◽  
Dependent Manner ◽  
Undifferentiated Hesc

Embryonic stem cells have pluripotency and differentiate into and constitute the cells and tissues of our body. In this study, using human embryonic stem cells (hESC), we evaluated novel methods for screening toxicological chemicals during developmental process. We elucidated developmental toxicity of two well-known chemicals, 5-fluorouracil (5-FU) and indomethacin (Indo) in hESC. The undifferentiated hESC were treated with the chemicals (10–4 to 104 µM of 5-FU and Indo) in a dose-dependent manner during 1 to 3 days. Surface markers (SSEA-4, TRA-1-60, and TRA-1-81) expressed only in undifferentiated hESC were monitored by immunocytochemistry to ensure the characterisation of undifferentiated hESC. Moreover, expression of embryonic stem cell-specific genes was assessed with real-time PCR after treatment of 5-FU and Indo (10–2, 100, and102 µM of 5-FU and Indo). The expression of surface markers was not significantly affected by treatment of 5-FU and Indo. The expression of transcription factors (Oct-4, Sox-2, Nanog, and hTERT) was significantly decreased by high concentrations of 5-FU and Indo (102 µM). However, no difference was observed in treatment of low concentration of 5-FU and Indo (10–2 µM). Taken together, these results suggest that 5-FU and Indo have cytotoxic effects, and modulate the expression of transcription factors that have pivotal roles in undifferentiated hESC. Therefore, we suggest that hESC may have potential to test toxicity of chemicals during embryonic developmental stage.

scholarly journals LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1

2013 ◽  
Vol 41 (16) ◽  
pp. 7753-7770 ◽  
Author(s):  
Pei-Shan Hou ◽  
Ching-Yu Chuang ◽  
Cheng-Fu Kao ◽  
Shen-Ju Chou ◽  
Lee Stone ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Transcriptional Modulation

scholarly journals FOXO 4 is necessary for neural differentiation of human embryonic stem cells

Aging Cell ◽  
2013 ◽  
Vol 12 (3) ◽  
pp. 518-522 ◽  
Author(s):  
David Vilchez ◽  
Leah Boyer ◽  
Margaret Lutz ◽  
Carsten Merkwirth ◽  
Ianessa Morantte ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem

scholarly journals Epigenetic Regulation of miR-302 by JMJD1C Inhibits Neural Differentiation of Human Embryonic Stem Cells

2013 ◽  
Vol 289 (4) ◽  
pp. 2384-2395 ◽  
Author(s):  
Jianle Wang ◽  
Jung W. Park ◽  
Hicham Drissi ◽  
Xiaofang Wang ◽  
Ren-He Xu
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Epigenetic Regulation ◽  
Neural Differentiation ◽  
Embryonic Stem
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