Production of amino acids by Azotobacter vinelandii and Azotobacter chroococcum with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions

ABSTRACT Encystment in Azotobacter vinelandii is induced byn-butanol or β-hydroxybutyrate (BHB). We identified a gene, encoding an aldehyde dehydrogenase, that was namedaldA. An aldA mutation impaired bacterial growth on n-butanol, ethanol, or hexanol as the sole carbon source. Expression of aldA increased in cells shifted from sucrose to n-butanol and was shown to be dependent on the alternative ς54 factor. A mutation in rpoNencoding the ς54 factor also impaired growth on alcohols. Encystment on n-butanol, but not on BHB, was impaired inaldA or rpoN mutants, indicating thatn-butanol is not an inducer of encystment by itself but must be catabolized in order to induce encystment.

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The effect of carbon source on trap formation by Arthrobotrys conoides

Canadian Journal of Microbiology ◽

10.1139/m68-210 ◽

1968 ◽

Vol 14 (11) ◽

pp. 1260-1261

Author(s):

A. W. James ◽

R. J. Nowakowski

Keyword(s):

Amino Acids ◽

Carbon Source ◽

Sole Carbon Source ◽

Major Effect ◽

Trap Formation ◽

Arthrobotrys Conoides

The nematode-trapping fungus Arthrobotrys conoides grows as typical vegetative hyphae on laboratory media containing glucose or starch as sole carbon source. However, in the presence of nematodes, "nemin", and several of the aliphatic amino acids (such as valine), this predacious fungus forms traps which are composed of sticky loops. The present studies show that, when A. conoides is grown on a semisynthetic medium in which various carbohydrates are substituted for glucose as the carbon source available for growth, the nature of the carbohydrate exerts a major effect on the induction of trap formation.

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Production of B-group vitamins by twoAzotobacterstrains with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions

Journal of Applied Microbiology ◽

10.1046/j.1365-2672.2000.01139.x ◽

2000 ◽

Vol 89 (3) ◽

pp. 486-493 ◽

Cited By ~ 47

Author(s):

J.J. Revillas ◽

B. Rodelas ◽

C. Pozo ◽

M.V. Martínez-Toledo ◽

J. González-López

Keyword(s):

Phenolic Compounds ◽

Carbon Source ◽

Sole Carbon Source

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Deletion of the GCW13 gene derepresses Gap1-dependent uptake of amino acids in Pichia pastoris grown on methanol as the sole carbon source

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2018.04.221 ◽

2018 ◽

Vol 501 (1) ◽

pp. 226-231 ◽

Cited By ~ 2

Author(s):

Chengjuan Zou ◽

Pan Wang ◽

Shuli Liang ◽

Shuangyan Han ◽

Suiping Zheng ◽

...

Keyword(s):

Amino Acids ◽

Pichia Pastoris ◽

Carbon Source ◽

Sole Carbon Source

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Constructing an ethanol utilization pathway in Escherichia coli to produce acetyl-CoA derived compounds

10.1101/2020.04.14.041889 ◽

2020 ◽

Author(s):

Hong Liang ◽

Xiaoqiang Ma ◽

Wenbo Ning ◽

Yurou Liu ◽

Anthony J. Sinskey ◽

...

Keyword(s):

Escherichia Coli ◽

Amino Acids ◽

Carbon Source ◽

Sole Carbon Source ◽

Structural Similarity ◽

List Type ◽

Acetyl Coa ◽

E Coli ◽

Utilization Pathway ◽

Aspartate Family

AbstractEngineering microbes to utilize non-conventional substrates could create short and efficient pathways to convert substrate into product. In this study, we designed and constructed a two-step heterologous ethanol utilization pathway (EUP) in Escherichia coli by using acetaldehyde dehydrogenase (encoded by ada) from Dickeya zeae and alcohol dehydrogenase (encoded by adh2) from Saccharomyces cerevisiae. This EUP can convert ethanol into acetyl-CoA without ATP consumption, and generate two molecules of NADH per molecule of ethanol. We optimized the expression of these two genes and found that ethanol consumption could be improved by expressing them in a specific order (ada-adh2) with a constitutive promoter (PgyrA). The engineered E. coli strain with EUP consumed approximately 8 g/L of ethanol in 96 hours when it was used as sole carbon source. Subsequently, we combined EUP with the biosynthesis of polyhydroxybutyrate (PHB), a biodegradable polymer derived from acetyl-CoA. The engineered E. coli strain carrying EUP and PHB biosynthetic pathway produced 1.1 g/L of PHB from 10 g/L of ethanol and 1 g/L of aspartate family amino acids in 96 hours. We also engineered E. coli strain to produced 24 mg/L of prenol from 10 g/L of ethanol in 48 hours, supporting the feasibility of converting ethanol into different classes of acetyl-CoA derived compounds.HighlightsEngineered Escherichia coli strains to grow on ethanol as sole carbon sourceDemonstrated that ethanol was converted into acetyl-CoA (AcCoA) through two pathways (acetaldehyde-acetate-AcCoA and acetaldehyde-AcCoA)Converted ethanol into two acetyl-CoA derived products with low structural similarity (polyhydroxybutyrate and prenol)Discovered that supplementation of the aspartate family amino acids can substantially improve cell growth on ethanol

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Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

Clinical Microbiology and Infection ◽

10.1016/j.cmi.2020.10.032 ◽

2020 ◽

Author(s):

Vivek Kumar Ranjan ◽

Shriparna Mukherjee ◽

Subarna Thakur ◽

Krutika Gupta ◽

Ranadhir Chakraborty

Keyword(s):

Carbon Source ◽

Sole Carbon Source ◽

New Delhi ◽

Pseudomonas Sp

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Phytochemical and Psychotropic Research of Motherwort (Leonurus cardiaca L.) Modified Dry Extracts

Plants ◽

10.3390/plants10020230 ◽

2021 ◽

Vol 10 (2) ◽

pp. 230

Author(s):

Oleh Koshovyi ◽

Ain Raal ◽

Igor Kireyev ◽

Nadiya Tryshchuk ◽

Tetiana Ilina ◽

...

Keyword(s):

Amino Acids ◽

Chemical Composition ◽

Phenolic Compounds ◽

Pharmacological Activity ◽

Ellagic Acid ◽

Hydroxycinnamic Acids ◽

Psychotropic Activity ◽

Phytochemical Analysis ◽

Phenolic Substances ◽

Leonurus Cardiaca

The prospect of creating a new medicine with psychotropic activity is shown as a result of studying the chemical composition and pharmacological activity of modified dry extracts of motherwort (Leonurus cardiaca L.) tincture. The most promising substances were the dry extracts, modified by adding small amounts of arginine, valine, phenylalanine, glycine, lysine, and alanine. A total of 15 main phenolic substances were found in the extracts, and eight of them were identified. There were also 10 hydroxycinnamic acids in these extracts, three of which were identified (chlorogenic, caffeic, and rosmarinic acids). The dominant hydroxycinnamic acids were chlorogenic and caffeic acids. Among flavonoids, catechin, hyperoside, and rutin were identified. It should be noted that the extracts had a significant content of ellagic acid. On the basis of the results of the phytochemical analysis of the extracts, it can be concluded that the composition of phenolic compounds does not differ significantly, and the main differences are related to amino acids, which obviously have an impact on the overall pharmacological effect. The results obtained indicate the presence of anxiolytic activity in the motherwort extracts studied in complex with amino acids. The extracts with glycine, valine, and arginine were more effective in reducing anxiety in animals.

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