YAF2 exerts anti-apoptotic effect in human tumor cells in a FANK1- and phosphorylation-dependent manner

2021 ◽  
Vol 554 ◽  
pp. 99-106
Author(s):  
Shiqiang Zhang ◽  
Xuan Zhang ◽  
Xin Guan ◽  
Xiaoli Ma ◽  
Hong Chen ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Dependent Manner ◽  
Human Tumor Cells ◽  
Apoptotic Effect

scholarly journals Casticin-Induced Inhibition of Cell Growth and Survival Are Mediated through the Dual Modulation of Akt/mTOR Signaling Cascade

Cancers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 254 ◽  
Author(s):  
Jong Lee ◽  
Chulwon Kim ◽  
Jae-Young Um ◽  
Gautam Sethi ◽  
Kwang Ahn
Keyword(s):  
Cell Proliferation ◽  
Tumor Cells ◽  
Human Tumor ◽  
Mtor Signaling ◽  
Signaling Cascade ◽  
Human Tumor Cells ◽  
Critical Pathway ◽  
Pharmacological Agents ◽  
Growth And Survival ◽  
Apoptotic Effect

The Akt/mTOR signaling cascade is a critical pathway involved in various physiological and pathological conditions, including regulation of cell proliferation, survival, invasion, and angiogenesis. In the present study, we investigated the anti-neoplastic effects of casticin (CTC), identified from the plant Vitex rotundifolia L., alone and/or in combination with BEZ-235, a dual Akt/mTOR inhibitor in human tumor cells. We found that CTC exerted a significant dose-dependent cytotoxicity and reduced cell proliferation in a variety of human tumor cells. Also, CTC effectively blocked the phosphorylation levels of Akt (Ser473) and mTOR (Ser2448) proteins as well as induced substantial apoptosis. Additionally treatment with CTC and BEZ-235 in conjunction resulted in a greater apoptotic effect than caused by either agent alone thus implicating the anti-neoplastic effects of this novel combination. Overall, the findings suggest that CTC can interfere with Akt/mTOR signaling cascade involved in tumorigenesis and can be used together with pharmacological agents targeting Akt/mTOR pathway.

Extraction, Purification and Cytotoxic Activity of Cerebrosides from Asterina pectinifera

2013 ◽  
Vol 781-784 ◽  
pp. 682-686 ◽  
Author(s):  
Hai Jiao Zuo ◽  
Tian Wang ◽  
Yan Xia Qi ◽  
Qian Cheng Zhao ◽  
Zhi Bo Li
Keyword(s):  
Thin Layer ◽  
Tumor Cells ◽  
Human Tumor ◽  
Time Dependent ◽  
Cytotoxic Activities ◽  
Dependent Manner ◽  
Human Tumor Cells ◽  
Ethanol Extraction ◽  
Asterina Pectinifera ◽  
Layer Chromatography

Cerebrosides were extracted from Asterina pectinifera using ethanol extraction and silica gel column chromatography. The identification of the cerebrosides was established based on thin layer chromatography (TLC), UV, IR and HRMS. The cerebrosides exhibited moderate cytotoxic activities against Hela and BEL7402 cell lines. At the concentration range of 10-400μg/ml, cytotoxicity of cerebrosides on two human tumor cells showed a dose-and time-dependent manner.

Inhibition by dihydrorifampicin of RNA polymerases I and II isolated from nuclei of Rous sarcoma cells and human tumor cells HEp-2

1979 ◽  
Vol 44 (9) ◽  
pp. 2722-2736 ◽  
Author(s):  
Jindřich Kára ◽  
Zdeněk Hostomský
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Tumor Cell Line ◽  
Selective Inhibition ◽  
Rna Polymerases ◽  
Embryonic Cells ◽  
Human Tumor Cells ◽  
Ribonucleic Acids ◽  
Rous Sarcoma ◽  
Sarcoma Cells

Dihydrorifampicin, a rifampicin derivative hydrogenated at the 18-19 carbon atoms of the aliphatic ansa chain of the rifampicin molecule, inhibits the enzymatic activity of RNA polymerases I and II, isolated from the nuclei of avian tumor cells (Rous sarcoma) and from the human tumor cell line HEp-2. The RNA polymerases from these tumors have been separated and partially purified by chromatography on DEAE Sephadex A-25 and characterized by the sensitivity to α-amanitin. The [3H]UMP-labeled ribonucleic acids synthesized in the isolated nuclei of Rous sarcoma cells in the presence and absence of DHR were analyzed by sedimentation analysis in sucrose density gradients. It was found that the synthesis of rRNAs and mRNAs is very significantly inhibited by dihydrorifampicin, whereas the synthesis of tRNAs is much less inhibited at the same DHR concentration (100μg/ml). The observed cytostatic effect of DHR on the growth of human tumor cells HEp-2 and embryonic cells in culture is apparently mediated by the selective inhibition of RNA polymerases I and II in human and avian cells. The relationship between the molecular structure of DHR and its affinity to RNA polymerases of eukaryotic cells is discussed.

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